ID   WAPI_BACNB              Reviewed;         119 AA.
AC   D4G3R3;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Immunity protein WapI;
GN   Name=wapI; ORFNames=BSNT_10604;
OS   Bacillus subtilis subsp. natto (strain BEST195).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=645657;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BEST195;
RX   PubMed=20398357; DOI=10.1186/1471-2164-11-243;
RA   Nishito Y., Osana Y., Hachiya T., Popendorf K., Toyoda A., Fujiyama A.,
RA   Itaya M., Sakakibara Y.;
RT   "Whole genome assembly of a natto production strain Bacillus subtilis natto
RT   from very short read data.";
RL   BMC Genomics 11:243-243(2010).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=BEST195;
RX   PubMed=25329997; DOI=10.1371/journal.pone.0109999;
RA   Kamada M., Hase S., Sato K., Toyoda A., Fujiyama A., Sakakibara Y.;
RT   "Whole genome complete resequencing of Bacillus subtilis natto by combining
RT   long reads with high-quality short reads.";
RL   PLoS ONE 9:E109999-E109999(2014).
RN   [3]
RP   FUNCTION AS AN IMMUNITY PROTEIN, AND EXPRESSION IN E.COLI.
RC   STRAIN=BEST195;
RX   PubMed=23572593; DOI=10.1073/pnas.1300627110;
RA   Koskiniemi S., Lamoureux J.G., Nikolakakis K.C., t'Kint de Roodenbeke C.,
RA   Kaplan M.D., Low D.A., Hayes C.S.;
RT   "Rhs proteins from diverse bacteria mediate intercellular competition.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:7032-7037(2013).
CC   -!- FUNCTION: Immunity protein component of a toxin-immunity protein
CC       module, which functions as a cellular contact-dependent growth
CC       inhibition (CDI) system. Neutralizes the tRNase activity of cognate
CC       toxin WapA upon expression in E.coli. Does not inhibit WapA from other
CC       strains of B.subtilis. The WapA C-terminus cannot be expressed on its
CC       own in E.coli, however it can be cloned in the presence of its cognate
CC       immunity protein gene. Cell contact is probably necessary for growth
CC       inhibition. {ECO:0000269|PubMed:23572593}.
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DR   EMBL; AP011541; BAI87616.1; -; Genomic_DNA.
DR   RefSeq; WP_014481370.1; NC_017196.2.
DR   AlphaFoldDB; D4G3R3; -.
DR   EnsemblBacteria; BAI87616; BAI87616; BSNT_10604.
DR   KEGG; bso:BSNT_10604; -.
DR   PATRIC; fig|645657.3.peg.830; -.
DR   HOGENOM; CLU_154373_0_0_9; -.
DR   Proteomes; UP000006805; Chromosome.
DR   InterPro; IPR025551; WapI-like.
DR   Pfam; PF14176; YxiJ; 1.
PE   1: Evidence at protein level;
FT   CHAIN           1..119
FT                   /note="Immunity protein WapI"
FT                   /id="PRO_0000423977"
SQ   SEQUENCE   119 AA;  14709 MW;  EA819A5045373CBD CRC64;
     MKFFKRYNID QKTLDEFKKY YVLLHGPFPN DMYDFEEETN TSLDEFYEFF ALITGSLNYI
     IEDKKIPRYQ REMLKKTFYE HYPHFRNYKS DILKYQELSE CLEFHEKIRI LINKLITGG