ID   WAPI_BACS4              Reviewed;         306 AA.
AC   G4NYJ5;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Immunity protein WapI;
GN   Name=wapI; OrderedLocusNames=GYO_4329;
OS   Bacillus spizizenii (strain DSM 15029 / JCM 12233 / NBRC 101239 / NRRL
OS   B-23049 / TU-B-10) (Bacillus subtilis subsp. spizizenii).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1052585;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15029 / JCM 12233 / NBRC 101239 / NRRL B-23049 / TU-B-10;
RX   PubMed=22493193; DOI=10.1128/jb.05675-11;
RA   Earl A.M., Eppinger M., Fricke W.F., Rosovitz M.J., Rasko D.A.,
RA   Daugherty S., Losick R., Kolter R., Ravel J.;
RT   "Whole-genome sequences of Bacillus subtilis and close relatives.";
RL   J. Bacteriol. 194:2378-2379(2012).
RN   [2]
RP   FUNCTION AS AN IMMUNITY PROTEIN, AND EXPRESSION IN E.COLI.
RC   STRAIN=DSM 15029 / JCM 12233 / NBRC 101239 / NRRL B-23049 / TU-B-10;
RX   PubMed=23572593; DOI=10.1073/pnas.1300627110;
RA   Koskiniemi S., Lamoureux J.G., Nikolakakis K.C., t'Kint de Roodenbeke C.,
RA   Kaplan M.D., Low D.A., Hayes C.S.;
RT   "Rhs proteins from diverse bacteria mediate intercellular competition.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:7032-7037(2013).
CC   -!- FUNCTION: Immunity protein component of a toxin-immunity protein
CC       module, which functions as a cellular contact-dependent growth
CC       inhibition (CDI) system. Neutralizes the tRNase activity of cognate
CC       toxin WapA upon expression in E.coli. Does not inhibit WapA from other
CC       strains of B.subtilis. The WapA C-terminus cannot be expressed on its
CC       own in E.coli, however it can be cloned in the presence of its cognate
CC       immunity protein gene. Cell contact is probably necessary for growth
CC       inhibition. {ECO:0000269|PubMed:23572593}.
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DR   EMBL; CP002905; AEP88888.1; -; Genomic_DNA.
DR   AlphaFoldDB; G4NYJ5; -.
DR   EnsemblBacteria; AEP88888; AEP88888; GYO_4329.
DR   KEGG; bst:GYO_4329; -.
DR   HOGENOM; CLU_961918_0_0_9; -.
DR   OMA; YRSMGED; -.
DR   Proteomes; UP000002651; Chromosome.
PE   1: Evidence at protein level;
FT   CHAIN           1..306
FT                   /note="Immunity protein WapI"
FT                   /id="PRO_0000423978"
SQ   SEQUENCE   306 AA;  35616 MW;  68D7CCFEFBB159B0 CRC64;
     MILSSFLKIE RMSSMHELKF RDLNVEVNEE DTVLLSMTES EIIVLTKKEI DYGAKYINHI
     VLYCNTDGRF LNSFSIQTNE QVINVQKVDD SFLFLIDKEY EDSVRDVEPN IYLWNPIEGF
     HQSFYAGRYI NSMIIDQNKN LWVGYDETGI FSCVDQEIST RGINKFVLKN GKYELYFHGV
     SSYVIDQYFS TFVSEDAIYL YYRSMGEDYL QKLNLLGETL ERVEVGIECS SCIKNGSSIY
     LFSRDDDSYN IEKVFKTNDM QNYVEQKISN ENNGESLCFT QVASYKDKVA GIDHNNKLFL
     LNNQSL