WAPI_BACSU
ID WAPI_BACSU Reviewed; 142 AA.
AC Q07836;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Immunity protein WapI;
GN Name=wapI; Synonyms=yxxG; OrderedLocusNames=BSU39220; ORFNames=N17H;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8316082; DOI=10.1111/j.1365-2958.1993.tb01574.x;
RA Foster S.J.;
RT "Molecular analysis of three major wall-associated proteins of Bacillus
RT subtilis 168: evidence for processing of the product of a gene encoding a
RT 258 kDa precursor two-domain ligand-binding protein.";
RL Mol. Microbiol. 8:299-310(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=7704263; DOI=10.1099/13500872-141-2-337;
RA Yoshida K., Sano H., Seki S., Oda M., Fujimura M., Fujita Y.;
RT "Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome
RT containing the hut and wapA loci.";
RL Microbiology 141:337-343(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=8969509; DOI=10.1099/13500872-142-11-3113;
RA Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y.,
RA Fujita Y.;
RT "Sequencing of a 65 kb region of the Bacillus subtilis genome containing
RT the lic and cel loci, and creation of a 177 kb contig covering the gnt-
RT sacXY region.";
RL Microbiology 142:3113-3123(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP FUNCTION AS AN IMMUNITY PROTEIN, EXPRESSION IN E.COLI, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=168;
RX PubMed=23572593; DOI=10.1073/pnas.1300627110;
RA Koskiniemi S., Lamoureux J.G., Nikolakakis K.C., t'Kint de Roodenbeke C.,
RA Kaplan M.D., Low D.A., Hayes C.S.;
RT "Rhs proteins from diverse bacteria mediate intercellular competition.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:7032-7037(2013).
RN [6]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=34280190; DOI=10.1371/journal.pgen.1009682;
RA Kobayashi K.;
RT "Diverse LXG toxin and antitoxin systems specifically mediate intraspecies
RT competition in Bacillus subtilis biofilms.";
RL PLoS Genet. 17:e1009682-e1009682(2021).
CC -!- FUNCTION: Immunity protein component of a toxin-immunity protein
CC module, which functions as a cellular contact-dependent growth
CC inhibition (CDI) system. Neutralizes the tRNase activity of cognate
CC toxin WapA upon expression in E.coli. Does not inhibit WapA from other
CC strains of B.subtilis. The WapA C-terminus cannot be expressed on its
CC own in E.coli, however it can be cloned in the presence of its cognate
CC immunity protein gene. Cell contact is necessary for growth inhibition
CC (PubMed:23572593). Unlike the LXG toxin-immunity modules, WapAI
CC mediates competition under shaking culture conditions
CC (PubMed:34280190). {ECO:0000269|PubMed:23572593,
CC ECO:0000269|PubMed:34280190}.
CC -!- INDUCTION: Constitutively and highly expressed on solid and in liquid
CC medium, with or without biofilm formation by 12 hours of culture.
CC Repressed by DegU. {ECO:0000269|PubMed:34280190}.
CC -!- DISRUPTION PHENOTYPE: A double wapA-wapI deletion strain is growth
CC inhibited when cocultured with wild-type cells. When wapI is
CC reintroduced it restores growth in a cognate toxin-dependent fashion
CC (PubMed:23572593). Growth inhibition by wild-type cells is strongest on
CC LB medium and less effective on media that promote biofilm formation
CC (MSgg and 2xSGG) (PubMed:34280190). {ECO:0000269|PubMed:23572593,
CC ECO:0000269|PubMed:34280190}.
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DR EMBL; L05634; AAA22884.1; -; Genomic_DNA.
DR EMBL; D31856; BAA06657.1; -; Genomic_DNA.
DR EMBL; D29985; BAA06261.1; -; Genomic_DNA.
DR EMBL; D83026; BAA11684.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15958.1; -; Genomic_DNA.
DR PIR; S32921; S32921.
DR RefSeq; NP_391801.1; NC_000964.3.
DR RefSeq; WP_003227169.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; Q07836; -.
DR IntAct; Q07836; 1.
DR MINT; Q07836; -.
DR STRING; 224308.BSU39220; -.
DR jPOST; Q07836; -.
DR PaxDb; Q07836; -.
DR PRIDE; Q07836; -.
DR EnsemblBacteria; CAB15958; CAB15958; BSU_39220.
DR GeneID; 937508; -.
DR KEGG; bsu:BSU39220; -.
DR PATRIC; fig|224308.179.peg.4246; -.
DR eggNOG; ENOG502ZT8F; Bacteria.
DR OMA; YKLNCNE; -.
DR BioCyc; BSUB:BSU39220-MON; -.
DR Proteomes; UP000001570; Chromosome.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..142
FT /note="Immunity protein WapI"
FT /id="PRO_0000050045"
SQ SEQUENCE 142 AA; 16429 MW; E50DB43756304DC3 CRC64;
MAKIKDDCIE LELTPRRYQE LDDDPFILSV FELLENKKAV VRDFSAVLLE SEYKVLISGI
ETMIKGNQDS ISLETIEPFL FLSIDQENGN YRIKIKIIFD DYKESKSNSN LFEINCNEEK
LESFVTALKL NLENTKNPSK LP
