ID   WAPL1_ARATH             Reviewed;         930 AA.
AC   F4I7C7; O04085;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Wings apart-like protein 1 {ECO:0000303|PubMed:25033056};
DE            Short=AtWAPL1 {ECO:0000303|PubMed:25033056};
GN   Name=WAPL1 {ECO:0000303|PubMed:25033056};
GN   OrderedLocusNames=At1g11060 {ECO:0000312|Araport:AT1G11060};
GN   ORFNames=T19D16.5 {ECO:0000312|EMBL:AAB65476.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=23028719; DOI=10.1371/journal.pone.0044981;
RA   Valentine M.E., Wolyniak M.J., Rutter M.T.;
RT   "Extensive phenotypic variation among allelic T-DNA inserts in Arabidopsis
RT   thaliana.";
RL   PLoS ONE 7:e44981-e44981(2012).
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Columbia;
RX   PubMed=25033056; DOI=10.1371/journal.pgen.1004497;
RA   De K., Sterle L., Krueger L., Yang X., Makaroff C.A.;
RT   "Arabidopsis thaliana WAPL is essential for the prophase removal of cohesin
RT   during meiosis.";
RL   PLoS Genet. 10:e1004497-e1004497(2014).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Columbia;
RX   PubMed=26813623; DOI=10.1105/tpc.15.00781;
RA   De K., Bolanos-Villegas P., Mitra S., Yang X., Homan G., Jauh G.-Y.,
RA   Makaroff C.A.;
RT   "The Opposing Actions of Arabidopsis CHROMOSOME TRANSMISSION FIDELITY7 and
RT   WINGS APART-LIKE1 and 2 Differ in Mitotic and Meiotic Cells.";
RL   Plant Cell 28:521-536(2016).
CC   -!- FUNCTION: Regulator of sister chromatid cohesion in meiosis which
CC       negatively regulates cohesin association with chromatin, acting as an
CC       antagonist of CTF7 (PubMed:25033056, PubMed:26813623). Cohesion ensures
CC       that chromosome partitioning is accurate in both meiotic and mitotic
CC       cells and plays an important role in DNA repair (By similarity).
CC       Essential for the prophase removal of cohesin during meiosis thus
CC       determining the timely release of meiotic cohesion (PubMed:25033056).
CC       Important for proper spindle attachment and assembly during meiosis
CC       (PubMed:25033056). Helps to prevent abnormal centromere association
CC       during prophase I in meiocytes (PubMed:25033056). Required for early
CC       embryonic patterning (PubMed:25033056). Also involved in chromosome
CC       segregation during mitosis (PubMed:25033056).
CC       {ECO:0000250|UniProtKB:Q7Z5K2, ECO:0000269|PubMed:25033056,
CC       ECO:0000269|PubMed:26813623}.
CC   -!- SUBUNIT: Interacts with the cohesin complex throughout the cell cycle.
CC       {ECO:0000250|UniProtKB:Q7Z5K2}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q7Z5K2}.
CC       Chromosome {ECO:0000250|UniProtKB:Q7Z5K2}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, buds and siliques.
CC       {ECO:0000269|PubMed:25033056}.
CC   -!- DISRUPTION PHENOTYPE: Fewer leaves, more flowers and higher germination
CC       rates (PubMed:23028719). Plants missing both WAPL1 and WAPL2 have
CC       relatively normal growth (slightly slower) and development but exhibit
CC       a significant reduction in male and female fertility (aborted pollen
CC       and ovules prior to fertilization and embryo defects in fertilized
CC       seed), due to blocked removal of cohesin from chromosomes during
CC       meiotic prophase (late zygotene/pachytene stage) resulting in
CC       chromosome bridges, broken chromosomes and uneven chromosome
CC       segregation, and leading to shorter siliques containing fewer seeds;
CC       this double mutant restores pollen viablitity to pollen-lethal ctf7
CC       mutation (PubMed:25033056, PubMed:26813623). During mitosis, abnormal
CC       chromosome segregation but normal cohesin release (PubMed:25033056).
CC       {ECO:0000269|PubMed:23028719, ECO:0000269|PubMed:25033056,
CC       ECO:0000269|PubMed:26813623}.
CC   -!- SIMILARITY: Belongs to the WAPL family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB65476.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; U95973; AAB65476.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE28678.1; -; Genomic_DNA.
DR   PIR; E86244; E86244.
DR   RefSeq; NP_172573.2; NM_100979.4.
DR   AlphaFoldDB; F4I7C7; -.
DR   SMR; F4I7C7; -.
DR   STRING; 3702.AT1G11060.1; -.
DR   PaxDb; F4I7C7; -.
DR   PRIDE; F4I7C7; -.
DR   ProteomicsDB; 207595; -.
DR   EnsemblPlants; AT1G11060.1; AT1G11060.1; AT1G11060.
DR   GeneID; 837647; -.
DR   Gramene; AT1G11060.1; AT1G11060.1; AT1G11060.
DR   KEGG; ath:AT1G11060; -.
DR   Araport; AT1G11060; -.
DR   TAIR; locus:2197399; AT1G11060.
DR   eggNOG; KOG2152; Eukaryota.
DR   HOGENOM; CLU_015006_0_0_1; -.
DR   InParanoid; F4I7C7; -.
DR   OMA; SQDSVHW; -.
DR   OrthoDB; 554620at2759; -.
DR   PRO; PR:F4I7C7; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; F4I7C7; baseline and differential.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0009793; P:embryo development ending in seed dormancy; IGI:TAIR.
DR   GO; GO:1990571; P:meiotic centromere clustering; IMP:UniProtKB.
DR   GO; GO:0045132; P:meiotic chromosome segregation; IGI:TAIR.
DR   GO; GO:0010789; P:meiotic sister chromatid cohesion involved in meiosis I; IGI:TAIR.
DR   GO; GO:0045144; P:meiotic sister chromatid segregation; IMP:UniProtKB.
DR   GO; GO:0090306; P:meiotic spindle assembly; IMP:UniProtKB.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IGI:TAIR.
DR   GO; GO:0060623; P:regulation of chromosome condensation; IBA:GO_Central.
DR   GO; GO:0071922; P:regulation of cohesin loading; IMP:UniProtKB.
DR   Gene3D; 1.25.10.10; -; 2.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR039874; WAPL.
DR   InterPro; IPR022771; WAPL_C.
DR   PANTHER; PTHR22100; PTHR22100; 2.
DR   Pfam; PF07814; WAPL; 2.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Chromosome; Chromosome partition; Meiosis;
KW   Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..930
FT                   /note="Wings apart-like protein 1"
FT                   /id="PRO_0000450125"
FT   DOMAIN          854..909
FT                   /note="WAPL"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00603"
FT   REGION          540..566
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   930 AA;  103633 MW;  FBBB689E676E0EAD CRC64;
     MIIVKLTANR ICCSLLQLRR SYEHFYIFVF LPEIPLFRFS HLKLFPKNLQ IQRLVSAMME
     RTYGRRKPGI PRTLSDSLND SVSQTEYLSS SSSPDIEPID YSLLPFSSQE SSSLWHSSSR
     SNFREDYPQN GGVVRRAKRV RNGAEAAAFT STLLEAQEFG ELMEHEDEVN FALDGLRKGH
     QLRIRRASLS SLLSICASQH QRRSLRAQGI SQSIIDAILV LSLDDIPSNL AAATLFFALT
     ADGQDEHFME SPKCIKFLIK LLKPVIVTST EGKPRNIGFK LLSLLKDVDA ARDPVKMDDP
     SSSDILSRVQ ELLVNCKEMR LNDSYITETT RPELSTKWVA LLAMERACVS KISFDDTSGS
     VKKTGGNFKE KLRELGGLDA VLEVVMDCHA VMERWVEYDA LSVQEKKDNL HKQSLMLLLK
     CLKIMENATF LSTDNQNHLL GFKKCLGSHD SRMSFTELTI SVIKMLSGLH LRGGFSSPNT
     NNVNSHYSNG GNHDSVLEAN RKVTNEVVTI SSDTYSTVGS ISTRNGSVSQ RSQSIIHLDF
     SPTSMSGSQS SVSGNEPTTS KTRVGSTISG SFAGRLASLG SDIARTTLRT TQAGEPICKK
     FGEFAPPEES EDPFAFDLED YKPSKWAVVS VNQKKSRAQK KKGCYKQSKD ESLYQLFSSQ
     EESSNHRLNS QEESSNRDCS TSLQPSHCTN DIDEECLCLL FDCLLTAVKV LMNLTNDNVV
     GCRQVGGCRG LESMAELIAR HFPSFTRSQL FSEMEKTGSS HQKKDKYLTD QELDFLVAIL
     GLLVNLVERD GVNRSRLASA SVPITKPEEL QESEQEMIPL LCSIFLTNQG SAETKEETTT
     FTLDDEEAVL EGEKEAEKMI VEAYSALLLA FLSTESRSIR NSIKDYLPKR NLAILVPVLE
     RFVAFHMTLN MIPPETHKAV MGVIESCKSP