WAPL_DROME
ID WAPL_DROME Reviewed; 1741 AA.
AC Q9W517; O46095; Q24153; Q7KVZ8; Q8MRI2; Q9W518;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Protein wings apart-like;
DE AltName: Full=Protein parallel sister chromatids;
GN Name=wapl {ECO:0000312|EMBL:AAF45734.1};
GN Synonyms=pasc {ECO:0000303|PubMed:10747063}; ORFNames=CG3707;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAA91230.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RC TISSUE=Embryo {ECO:0000269|PubMed:10747063}, and Imaginal disk;
RX PubMed=10747063; DOI=10.1093/genetics/154.4.1693;
RA Verni F., Gandhi R., Goldberg M.L., Gatti M.;
RT "Genetic and molecular analysis of wings apart-like (wapl), a gene
RT controlling heterochromatin organization in Drosophila melanogaster.";
RL Genetics 154:1693-1710(2000).
RN [2] {ECO:0000312|EMBL:AAF45734.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAF45734.1}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:CAB10973.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R {ECO:0000269|PubMed:10731137};
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [5] {ECO:0000305, ECO:0000312|EMBL:AAM50260.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM50260.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-258; SER-355 AND SER-888, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Has a role in female meiotic chromosome segregation in
CC females; proximal heterochromatin is involved in chromosome pairing
CC during female meiosis. Is a dominant suppressor of both white and
CC Stubble position-effect variegation (PEV), while it is a weak enhancer
CC of brown variegation. {ECO:0000269|PubMed:10747063}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A {ECO:0000269|PubMed:10747063};
CC IsoId=Q9W517-1; Sequence=Displayed;
CC Name=B {ECO:0000303|PubMed:10731132};
CC IsoId=Q9W517-2; Sequence=VSP_051920;
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos.
CC {ECO:0000269|PubMed:10747063}.
CC -!- DISRUPTION PHENOTYPE: Flies exhibit brain cell metaphases where sister
CC chromatids of all chromosomes are aligned parallel to each other
CC instead of assuming the typical morphology, heterochromatin
CC condensation or chromosome segregation are not affected.
CC {ECO:0000269|PubMed:10747063}.
CC -!- SIMILARITY: Belongs to the WAPL family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA91230.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAM50260.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U40214; AAA91230.1; ALT_FRAME; mRNA.
DR EMBL; AE014298; AAF45734.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45735.2; -; Genomic_DNA.
DR EMBL; Z98269; CAB10973.1; -; Genomic_DNA.
DR EMBL; AY119606; AAM50260.1; ALT_FRAME; mRNA.
DR PIR; T13349; T13349.
DR PIR; T13610; T13610.
DR RefSeq; NP_001284804.1; NM_001297875.1. [Q9W517-1]
DR RefSeq; NP_525042.2; NM_080303.3. [Q9W517-1]
DR RefSeq; NP_726796.2; NM_166931.2. [Q9W517-2]
DR AlphaFoldDB; Q9W517; -.
DR SMR; Q9W517; -.
DR BioGRID; 57728; 2.
DR IntAct; Q9W517; 1.
DR STRING; 7227.FBpp0070413; -.
DR iPTMnet; Q9W517; -.
DR PaxDb; Q9W517; -.
DR EnsemblMetazoa; FBtr0070429; FBpp0070413; FBgn0004655. [Q9W517-1]
DR EnsemblMetazoa; FBtr0070430; FBpp0070414; FBgn0004655. [Q9W517-2]
DR EnsemblMetazoa; FBtr0344159; FBpp0310572; FBgn0004655. [Q9W517-1]
DR GeneID; 31187; -.
DR KEGG; dme:Dmel_CG3707; -.
DR CTD; 23063; -.
DR FlyBase; FBgn0004655; wapl.
DR VEuPathDB; VectorBase:FBgn0004655; -.
DR eggNOG; KOG2152; Eukaryota.
DR GeneTree; ENSGT00390000015768; -.
DR HOGENOM; CLU_001745_0_0_1; -.
DR InParanoid; Q9W517; -.
DR OMA; IQQLPHC; -.
DR PhylomeDB; Q9W517; -.
DR Reactome; R-DME-2468052; Establishment of Sister Chromatid Cohesion.
DR Reactome; R-DME-2470946; Cohesin Loading onto Chromatin.
DR Reactome; R-DME-2500257; Resolution of Sister Chromatid Cohesion.
DR BioGRID-ORCS; 31187; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31187; -.
DR PRO; PR:Q9W517; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0004655; Expressed in cleaving embryo and 24 other tissues.
DR ExpressionAtlas; Q9W517; baseline and differential.
DR Genevisible; Q9W517; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0006325; P:chromatin organization; IMP:UniProtKB.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0061781; P:mitotic cohesin unloading; IMP:FlyBase.
DR GO; GO:0060623; P:regulation of chromosome condensation; IBA:GO_Central.
DR GO; GO:0071922; P:regulation of cohesin loading; IBA:GO_Central.
DR GO; GO:0007062; P:sister chromatid cohesion; IMP:FlyBase.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR039874; WAPL.
DR InterPro; IPR022771; WAPL_C.
DR InterPro; IPR012502; WAPL_dom.
DR PANTHER; PTHR22100; PTHR22100; 2.
DR Pfam; PF07814; WAPL; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51271; WAPL; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome partition; Meiosis; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1741
FT /note="Protein wings apart-like"
FT /id="PRO_0000076364"
FT DOMAIN 1140..1648
FT /note="WAPL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00603"
FT REGION 68..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 291..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 490..612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 675..810
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 823..963
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1038..1068
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1112..1141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1708..1741
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..83
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..233
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..260
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..333
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 349..380
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..456
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 530..612
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 845..860
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 871..898
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 899..917
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1046..1068
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 258
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 355
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 888
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..649
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10731132"
FT /id="VSP_051920"
FT CONFLICT 37
FT /note="W -> L (in Ref. 5; AAM50260)"
FT /evidence="ECO:0000305"
FT CONFLICT 135
FT /note="A -> V (in Ref. 4; CAB10973)"
FT /evidence="ECO:0000305"
FT CONFLICT 772
FT /note="T -> M (in Ref. 4; CAB10973)"
FT /evidence="ECO:0000305"
FT CONFLICT 950
FT /note="A -> T (in Ref. 4; CAB10973)"
FT /evidence="ECO:0000305"
FT CONFLICT 1137
FT /note="V -> I (in Ref. 4; CAB10973)"
FT /evidence="ECO:0000305"
FT CONFLICT 1270..1271
FT /note="ST -> RL (in Ref. 1; AAA91230)"
FT /evidence="ECO:0000305"
FT CONFLICT 1276
FT /note="P -> S (in Ref. 4; CAB10973)"
FT /evidence="ECO:0000305"
FT CONFLICT 1708
FT /note="T -> S (in Ref. 4; CAB10973)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1741 AA; 185156 MW; BD770C27B824DE93 CRC64;
MSRWGKNIVV PLDSLCKEKE NTNRPTVARS VGTVGKWGKM GFTSTRTYTL PAIHPMAAAA
AAAAAAASPS QSPASTQDHD PNDLSVSVPE PPKPKKFFKS RNTAPPEVIA QIIQQLPHCG
AGASPMRDHF SSAGAGAGGL TPTSGAQEAG GVKLKPGKGA SSAERKRKSP KKKAATTSAS
TPSTPGAFYG ASDRDGDGLS DPASEQPEQP SSASGKQKQK KPKEEKKLKP EAPPSRVLGR
ARKAVNYREV DEDERYPTPT KDLIIPKAGR QPAEVAATAT LAAASSEAFI SSTFGSPGSE
PSLPPPTSAP SASASTSSQL PSASGSASNP PSASRTPEHP PIVLRISKGT SRLVSTDSEE
PPSSSPAHQN QLNQLSVTEE EPAERSGDET VPASTPKITV KPLRPPTAAD SVDGSSAAVG
GASAGDSFEE RKSQSLEPNE DEEEEEEEED EEEEPPEINY CTVKISPDKP PKERLKLIIK
TDVIRNAIAK AAAAAESRSE KKSRSKKHKH KQLLAAGSGA APASGATPAE INSEFKTPSP
HLALSEANSQ QAQHTPSHLH QLHQLHPQRG SAVISPTTRS DHDFDSQSSV LGSISSKGNS
TPQLLAQAVQ EDSCVIRSRG SSVITSDLET SQHSSLVAPP SDIESRLESM MMTIDGAGTG
AASAVPETPL QEDILAVLRG EVPRLNGNTD PEPTEEEDQQ QQPKRATRGR GRKANNNVDV
TPPATETRTR GRAKGADATT AAISPPTGKR NTRGTRGSRK AEQEVDMEVD ETAMTTVPAN
EEQLEQATLP PRRGRNAAAR ANNNNLASVN NNINKIAANL SAKAEASRLA EGGVAGGAAR
SYGRKRKNQQ VTQVLQQEPV PEEQETPDAE EEQPTPAKIP HTDHREHSPD HDPDPDPDEL
SNNSNNSSLQ HDGSSSSPPP RDFKFKDKFK RTLTLDTQGA ANAGAGGAAA AAPPESSGEQ
RGAVKLVISK KKGSIFKSRA LVPSDQAEQA TVAKRHLYKH SWDAALEANG GGTNSDASNA
SASGVGVAGA KDHLHHLAAG KSDGDFGDSP SSNNNGSSSA CSSASTLRGD SPALGKISRL
AGKQGVPATS TSSDAFDLDL EPIAGELDLE RSAAGASAGG TGATTGGGGA TGGGGPVRVD
RKTKDYYPVV RNVKTAHQIQ EIGEYQEMDD DVEYILDALQ PHNPPATRCL SALQLAAKCM
MPAFRMHVRA HGVVTKFFKA LSDANKDLSL GLCTSAIMYI LSQEGLNMDL DRDSLELMIN
LLEADGVGGS TETGHPDRAG YDRNKQKVRE LCEEIKAQGK GTHLNVDSLT VGTLAMETLL
SLTSKRAGEW FKEDLRKLGG LEHIIKTISD FCRPVIACDT EIDWQPTLLD NMQTVARCLR
VLENVTQHNE TNQRYMLTSG QGKAVETLCQ LYRLCSRQIM LHPSDGGGSN KEHPGVAMRE
LLVPVLKVLI NLTHTFNEAQ PSLGAELLGQ RGDVVETSFR LLLLSANYIP DQCVFELSIL
VLTLLINLCM HTVPNRAALM QAAAPAEYVA DNPPAQGSVS ALQALLEYFY KCEELARLVE
KNTDAFLESN EKGKKKQEEV EETVNNLVQR AGHHMEHTLK GSYAAILVGN LIADNELYES
VVRRQLRGNS FKEIIGVLEK YHTFMNLTSS LEAAFVAHMK STKRIIDNFK KRDYIYEHSD
EHDNPLPLNL ETTAQVLAVG ADASHAATSS TTVGSGSAPS STSATGTTRA PRVYKTYSSH
R
