WAPL_SCHPO
ID WAPL_SCHPO Reviewed; 602 AA.
AC O94364; Q9USF1;
DT 30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Wings apart-like protein homolog 1;
GN Name=wpl1; ORFNames=SPBC428.17c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-112, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP FUNCTION.
RX PubMed=18079700; DOI=10.1038/sj.emboj.7601955;
RA Bernard P., Schmidt C.K., Vaur S., Dheur S., Drogat J., Genier S.,
RA Ekwall K., Uhlmann F., Javerzat J.P.;
RT "Cell-cycle regulation of cohesin stability along fission yeast
RT chromosomes.";
RL EMBO J. 27:111-121(2008).
RN [5]
RP FUNCTION.
RX PubMed=21300781; DOI=10.1128/mcb.01284-10;
RA Feytout A., Vaur S., Genier S., Vazquez S., Javerzat J.P.;
RT "Psm3 acetylation on conserved lysine residues is dispensable for viability
RT in fission yeast but contributes to Eso1-mediated sister chromatid cohesion
RT by antagonizing Wpl1.";
RL Mol. Cell. Biol. 31:1771-1786(2011).
CC -!- FUNCTION: Regulator of sister chromatid cohesion in mitosis which
CC negatively regulates cohesin association with chromatin.
CC {ECO:0000269|PubMed:18079700, ECO:0000269|PubMed:21300781}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome.
CC -!- SIMILARITY: Belongs to the WAPL family. {ECO:0000305}.
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DR EMBL; CU329671; CAA22292.1; -; Genomic_DNA.
DR EMBL; AB027807; BAA87111.1; -; Genomic_DNA.
DR PIR; T40469; T40469.
DR RefSeq; NP_595195.1; NM_001021102.2.
DR AlphaFoldDB; O94364; -.
DR SMR; O94364; -.
DR BioGRID; 277377; 17.
DR STRING; 4896.SPBC428.17c.1; -.
DR iPTMnet; O94364; -.
DR MaxQB; O94364; -.
DR PaxDb; O94364; -.
DR PRIDE; O94364; -.
DR EnsemblFungi; SPBC428.17c.1; SPBC428.17c.1:pep; SPBC428.17c.
DR GeneID; 2540860; -.
DR KEGG; spo:SPBC428.17c; -.
DR PomBase; SPBC428.17c; wpl1.
DR VEuPathDB; FungiDB:SPBC428.17c; -.
DR eggNOG; ENOG502RS0D; Eukaryota.
DR HOGENOM; CLU_460162_0_0_1; -.
DR InParanoid; O94364; -.
DR OMA; YREQRSY; -.
DR Reactome; R-SPO-2470946; Cohesin Loading onto Chromatin.
DR Reactome; R-SPO-2500257; Resolution of Sister Chromatid Cohesion.
DR PRO; PR:O94364; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000228; C:nuclear chromosome; IC:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0090695; C:Wpl/Pds5 cohesin loading/unloading complex; EXP:PomBase.
DR GO; GO:0043008; F:ATP-dependent protein binding; IDA:PomBase.
DR GO; GO:0003677; F:DNA binding; IDA:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0061781; P:mitotic cohesin unloading; IDA:PomBase.
DR GO; GO:0060623; P:regulation of chromosome condensation; IBA:GO_Central.
DR GO; GO:0071922; P:regulation of cohesin loading; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR039874; WAPL.
DR InterPro; IPR022771; WAPL_C.
DR PANTHER; PTHR22100; PTHR22100; 1.
DR Pfam; PF07814; WAPL; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Chromosome; Mitosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..602
FT /note="Wings apart-like protein homolog 1"
FT /id="PRO_0000116778"
FT DOMAIN 160..492
FT /note="WAPL"
FT REGION 34..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 602 AA; 67570 MW; 33EB053CFCD7E093 CRC64;
MKRGKCKEKD NGLKRISSES EVWNFLDVTV SELNKQKRSP GQTVSKRLHK KQRVVSNPDL
SLPSSPVKQI LRNGLQNSKY GSHKTGLERS ASCSSIDASA NHSSTTYREQ RSYLMEEGLD
TQPIVPREVS SGRELDSTNH TIGTERAFLI EEDVSEDDEI QMKSIHELRF AGEQQRIVDE
IEYLVDGVTF SGNSSASRYL SLIGIAEKMF DNSFRLCLKS IRDVFLRIFE EIDPKDTLHT
FLQIYIFATM ANEMDCMSSL LDAYSNNVKL LLQTAITLEP QVPVSILAKS LPKSVKGAVQ
EFVIKAELTF SFSNESLASS DSISLAAIAL MKTSSGVFAE SELFTELINL LIEKSYPILK
ENDGSNNFLL HALCSSLEKF TDFQGSEKIQ KVSQILSSKL QILIDEHNET NSPKIDDTVV
HACSEKLLRT LIQTVNSNSD HALAVSKSEV PLFAYKILQK FSNFSSDDET TRELIILILG
LLLGLVEESH EFIQTITHVE VSFGASALDV LISFYQKNES IVEISGYVVM ILSHCFLNDP
KAFAQLKPLI SQFYESLHKF KNFHLKLKEE LMMMGSNGLA IVSIIDELHK SLQDYLRSDL
VK