ID   WAPN_NAJNG              Reviewed;          51 AA.
AC   P60589; P83769;
DT   15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2004, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Nawaprin;
OS   Naja nigricollis (Black-necked spitting cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8654;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, DISULFIDE BONDS, AND STRUCTURE BY NMR.
RC   TISSUE=Venom;
RX   PubMed=12878611; DOI=10.1074/jbc.m305322200;
RA   Torres A.M., Wong H.Y., Desai M., Moochhala S., Kuchel P.W., Kini R.M.;
RT   "Identification of a novel family of proteins in snake venoms. Purification
RT   and structural characterization of nawaprin from Naja nigricollis snake
RT   venom.";
RL   J. Biol. Chem. 278:40097-40104(2003).
CC   -!- FUNCTION: Damages membranes of susceptible bacteria. Has no hemolytic
CC       activity. Not toxic to mice. Does not inhibit the proteinases elastase
CC       and cathepsin G. {ECO:0000250|UniProtKB:P83952}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MASS SPECTROMETRY: Mass=5288.5; Mass_error=0.08; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:12878611};
CC   -!- SIMILARITY: Belongs to the snake waprin family. {ECO:0000305}.
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DR   PDB; 1UDK; NMR; -; A=1-51.
DR   PDBsum; 1UDK; -.
DR   AlphaFoldDB; P60589; -.
DR   SMR; P60589; -.
DR   MEROPS; I17.004; -.
DR   EvolutionaryTrace; P60589; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030414; F:peptidase inhibitor activity; IEA:InterPro.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.75.10; -; 1.
DR   InterPro; IPR036645; Elafin-like_sf.
DR   InterPro; IPR008197; WAP_dom.
DR   Pfam; PF00095; WAP; 1.
DR   SMART; SM00217; WAP; 1.
DR   SUPFAM; SSF57256; SSF57256; 1.
DR   PROSITE; PS51390; WAP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; Secreted.
FT   CHAIN           1..51
FT                   /note="Nawaprin"
FT                   /id="PRO_0000188988"
FT   DOMAIN          1..50
FT                   /note="WAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DISULFID        7..37
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:12878611"
FT   DISULFID        20..41
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:12878611"
FT   DISULFID        24..36
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:12878611"
FT   DISULFID        30..46
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:12878611"
FT   STRAND          4..6
FT                   /evidence="ECO:0007829|PDB:1UDK"
FT   HELIX           27..29
FT                   /evidence="ECO:0007829|PDB:1UDK"
FT   STRAND          35..37
FT                   /evidence="ECO:0007829|PDB:1UDK"
FT   STRAND          40..43
FT                   /evidence="ECO:0007829|PDB:1UDK"
FT   STRAND          45..47
FT                   /evidence="ECO:0007829|PDB:1UDK"
SQ   SEQUENCE   51 AA;  5296 MW;  27674128D7498CCC CRC64;
     NEKSGSCPDM SMPIPPLGIC KTLCNSDSGC PNVQKCCKNG CGFMTCTTPV P