ID   WAP_NOTEU               Reviewed;         191 AA.
AC   Q9N0L8;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Whey acidic protein;
DE   AltName: Full=tWAP;
DE   Flags: Precursor;
GN   Name=WAP;
OS   Notamacropus eugenii (Tammar wallaby) (Macropus eugenii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Metatheria; Diprotodontia; Macropodidae; Notamacropus.
OX   NCBI_TaxID=9315;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], 3D-STRUCTURE MODELING, AND DISULFIDE BONDS.
RC   TISSUE=Lactating mammary gland;
RX   PubMed=10801834; DOI=10.1074/jbc.m002161200;
RA   Simpson K.J., Ranganathan S., Fisher J.A., Janssens P.A., Shaw D.C.,
RA   Nicholas K.R.;
RT   "The gene for a novel member of the whey acidic protein family encodes
RT   three four-disulfide core domains and is asynchronously expressed during
RT   lactation.";
RL   J. Biol. Chem. 275:23074-23081(2000).
CC   -!- FUNCTION: Could be a protease inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Milk-specific; major protein component of milk
CC       whey.
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DR   EMBL; AJ005356; CAB90357.1; -; mRNA.
DR   AlphaFoldDB; Q9N0L8; -.
DR   SMR; Q9N0L8; -.
DR   MEROPS; I17.950; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030414; F:peptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0010466; P:negative regulation of peptidase activity; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.75.10; -; 3.
DR   InterPro; IPR036645; Elafin-like_sf.
DR   InterPro; IPR008197; WAP_dom.
DR   Pfam; PF00095; WAP; 3.
DR   PRINTS; PR00003; 4DISULPHCORE.
DR   SMART; SM00217; WAP; 3.
DR   SUPFAM; SSF57256; SSF57256; 3.
DR   PROSITE; PS51390; WAP; 3.
PE   1: Evidence at protein level;
KW   Disulfide bond; Glycoprotein; Milk protein; Protease inhibitor; Repeat;
KW   Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..191
FT                   /note="Whey acidic protein"
FT                   /id="PRO_0000041354"
FT   DOMAIN          22..71
FT                   /note="WAP 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DOMAIN          72..125
FT                   /note="WAP 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DOMAIN          126..175
FT                   /note="WAP 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT   CARBOHYD        69
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        29..59
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        42..63
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        46..58
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        52..67
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        78..114
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        97..118
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        101..113
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        107..122
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        133..163
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        140..167
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        150..162
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
FT   DISULFID        156..171
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722,
FT                   ECO:0000269|PubMed:10801834"
SQ   SEQUENCE   191 AA;  21133 MW;  BF90B81DADBBE50D CRC64;
     MQPVQILTLV LLALGAWAAQ ESTEKAGYCP DFRQVLLDRR DCKQLCNDDA SCPQNMRCCQ
     RGCSWLCMNT TQEKDGLCPV ATSHSSSSEE QQRKQLCDKT CKTDLGCEGK AKCCASSCGQ
     TCFMPVKAKP GRCPAVTGIC PEKKSWFHTC QRDDQCKENK KCCSSACGRR CTNPFPEEYE
     ASQDESTLLA L