WAP_RAT
ID WAP_RAT Reviewed; 137 AA.
AC P01174;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 2.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=Whey acidic protein;
DE Short=WAP;
DE AltName: Full=Whey phosphoprotein;
DE Flags: Precursor;
GN Name=Wap;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6095207; DOI=10.1093/nar/12.22.8685;
RA Campbell S.M., Rosen J.M., Hennighausen L.G., Strech-Jurk U., Sippel A.E.;
RT "Comparison of the whey acidic protein genes of the rat and mouse.";
RL Nucleic Acids Res. 12:8685-8697(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=6896749; DOI=10.1093/nar/10.12.3733;
RA Hennighausen L.G., Sippel A.E.;
RT "Comparative sequence analysis of the mRNAs coding for mouse and rat whey
RT protein.";
RL Nucleic Acids Res. 10:3733-3744(1982).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-41, AND DISULFIDE BONDS.
RX PubMed=6955785; DOI=10.1073/pnas.79.13.3987;
RA Dandekar A.M., Robinson E.A., Appella E., Qasba P.K.;
RT "Complete sequence analysis of cDNA clones encoding rat whey
RT phosphoprotein: homology to a protease inhibitor.";
RL Proc. Natl. Acad. Sci. U.S.A. 79:3987-3991(1982).
CC -!- FUNCTION: Could be a protease inhibitor.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Milk-specific; major protein component of milk
CC whey.
CC -!- PTM: Contains 8 disulfide bonds.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X01153; CAA25600.2; -; Genomic_DNA.
DR EMBL; X01154; CAA25600.2; JOINED; Genomic_DNA.
DR EMBL; X01155; CAA25600.2; JOINED; Genomic_DNA.
DR EMBL; X01156; CAA25600.2; JOINED; Genomic_DNA.
DR EMBL; J00802; AAA42347.1; -; mRNA.
DR EMBL; J00801; AAA42346.1; -; mRNA.
DR PIR; A93920; WYRT.
DR RefSeq; NP_446203.1; NM_053751.4.
DR AlphaFoldDB; P01174; -.
DR SMR; P01174; -.
DR STRING; 10116.ENSRNOP00000010744; -.
DR PaxDb; P01174; -.
DR GeneID; 114596; -.
DR KEGG; rno:114596; -.
DR UCSC; RGD:621851; rat.
DR CTD; 22373; -.
DR RGD; 621851; Wap.
DR eggNOG; ENOG502RXHY; Eukaryota.
DR InParanoid; P01174; -.
DR OrthoDB; 1409658at2759; -.
DR PhylomeDB; P01174; -.
DR PRO; PR:P01174; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0030414; F:peptidase inhibitor activity; NAS:RGD.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR Gene3D; 4.10.75.10; -; 1.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR008197; WAP_dom.
DR Pfam; PF00095; WAP; 1.
DR PRINTS; PR00003; 4DISULPHCORE.
DR SMART; SM00217; WAP; 1.
DR SUPFAM; SSF57256; SSF57256; 1.
DR PROSITE; PS51390; WAP; 2.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Milk protein;
KW Protease inhibitor; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:6955785"
FT CHAIN 20..137
FT /note="Whey acidic protein"
FT /id="PRO_0000041353"
FT DOMAIN 27..73
FT /note="WAP 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DOMAIN 76..127
FT /note="WAP 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 34..61
FT /evidence="ECO:0000305|PubMed:6955785"
FT DISULFID 45..65
FT /evidence="ECO:0000305|PubMed:6955785"
FT DISULFID 48..60
FT /evidence="ECO:0000305|PubMed:6955785"
FT DISULFID 54..69
FT /evidence="ECO:0000305|PubMed:6955785"
FT DISULFID 83..115
FT /evidence="ECO:0000305|PubMed:6955785"
FT DISULFID 96..119
FT /evidence="ECO:0000305|PubMed:6955785"
FT DISULFID 102..114
FT /evidence="ECO:0000305|PubMed:6955785"
FT DISULFID 108..123
FT /evidence="ECO:0000305|PubMed:6955785"
FT CONFLICT 4
FT /note="S -> F (in Ref. 3; AAA42347)"
FT /evidence="ECO:0000305"
FT CONFLICT 35
FT /note="S -> P (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="F -> S (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="N -> K (in Ref. 2; AAA42346)"
FT /evidence="ECO:0000305"
FT CONFLICT 68
FT /note="S -> P (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 99
FT /note="D -> G (in Ref. 2; AAA42346)"
FT /evidence="ECO:0000305"
FT CONFLICT 116
FT /note="K -> N (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 127
FT /note="E -> K (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="K -> D (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="K -> E (in Ref. 3; AAA42347)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="I -> V (in Ref. 3; AAA42347)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 137 AA; 14827 MW; 1C2E8ADA9FD97949 CRC64;
MRCSISLVLG LLALEVALAR NLQEHVFNSV QSMCSDDSFS EDTECINCQT NEECAQNDMC
CPSSCGRSCK TPVNIEVQKA GRCPWNPIQM IAAGPCPKDN PCSIDSDCSG TMKCCKNGCI
MSCMDPEPKS PTVISFQ
