WASC2_DICDI
ID WASC2_DICDI Reviewed; 1479 AA.
AC Q552E2; Q75JI1;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=WASH complex subunit 2 {ECO:0000250|UniProtKB:Q9Y4E1};
GN Name=washc2 {ECO:0000250|UniProtKB:Q9Y4E1}; Synonyms=fam21;
GN ORFNames=DDB_G0276221;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP PROTEIN SEQUENCE OF 2-39; 65-80; 86-105; 141-151; 296-315; 379-416;
RP 442-461; 475-495; 497-551; 565-580; 595-610; 617-635; 644-690; 699-726;
RP 731-768; 778-823; 832-847; 884-904; 945-982; 987-1008; 1072-1094;
RP 1119-1131; 1165-1180; 1247-1283; 1292-1319; 1402-1424; 1442-1454 AND
RP 1471-1479, CLEAVAGE OF INITIATOR METHIONINE, PHOSPHORYLATION AT THR-535 AND
RP SER-1249, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=AX2;
RA Bienvenut W.V., Ura S., Insall R.H.;
RL Submitted (JUL-2009) to UniProtKB.
RN [4]
RP PROTEIN SEQUENCE OF 66-79; 140-150; 418-435; 564-579; 617-634; 730-761;
RP 1441-1455 AND 1470-1478, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=AX2;
RA Bienvenut W.V., Sumpton D., Ura S., Insall R.H.;
RL Submitted (OCT-2008) to UniProtKB.
CC -!- SUBUNIT: Probable component of the WASH complex.
CC {ECO:0000250|UniProtKB:Q9Y4E1}.
CC -!- SIMILARITY: Belongs to the FAM21 family. {ECO:0000305}.
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DR EMBL; AAFI02000014; EAL69410.1; -; Genomic_DNA.
DR RefSeq; XP_643271.1; XM_638179.1.
DR AlphaFoldDB; Q552E2; -.
DR STRING; 44689.DDB0232153; -.
DR PaxDb; Q552E2; -.
DR EnsemblProtists; EAL69410; EAL69410; DDB_G0276221.
DR GeneID; 8620314; -.
DR KEGG; ddi:DDB_G0276221; -.
DR dictyBase; DDB_G0276221; fam21.
DR eggNOG; ENOG502QTIY; Eukaryota.
DR HOGENOM; CLU_249774_0_0_1; -.
DR InParanoid; Q552E2; -.
DR OMA; IHTIFYD; -.
DR PRO; PR:Q552E2; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005769; C:early endosome; IBA:GO_Central.
DR GO; GO:0071203; C:WASH complex; IDA:dictyBase.
DR GO; GO:1901981; F:phosphatidylinositol phosphate binding; IBA:GO_Central.
DR GO; GO:1905394; F:retromer complex binding; IBA:GO_Central.
DR GO; GO:0006887; P:exocytosis; IMP:dictyBase.
DR GO; GO:0036010; P:protein localization to endosome; IBA:GO_Central.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IBA:GO_Central.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.3"
FT CHAIN 2..1479
FT /note="WASH complex subunit 2"
FT /id="PRO_0000388365"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 188..210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 240..564
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 576..1383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1419..1479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..280
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 285..306
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..331
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 333..347
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 355..385
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 386..415
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 460..477
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 478..492
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 515..554
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 596..616
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 630..647
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 682..705
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 711..728
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 735..767
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 768..782
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 825..889
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 901..924
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 937..951
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 952..974
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 978..993
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1005..1044
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1059..1094
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1104..1124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1149..1191
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1217..1236
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1245..1296
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1304..1323
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1329..1383
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1419..1470
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 535
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|Ref.3"
FT MOD_RES 1249
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|Ref.3"
SQ SEQUENCE 1479 AA; 160367 MW; A7C7177F5EB19109 CRC64;
MPEEQPQQQQ QPVREQPSNP DDVPWKKVWT INEMRDSAKD WNLSSDAGLL NYMQDFANKL
IYKTKELEFQ VDELVKDSKS IHVKVHNTFD QFLMLSNTQF IENRVYEDNS ELNNEQNEQT
DKPQQQEITE EILIPKFSKA ILIGLEALEK ANFVNAALEI SEQSNSNNNN NDYQQQMNGS
IAASSAEGGL VEGGEQAGTD AQPSANTEKK KEKLDHYLRY PLPHIIGSQQ FHQDDFCGLF
IEDSDSDSSD EEDEEDVDAE DGSDESSSES SSDDDDEKDE QQQSTLFLTD EQPKQQNFFF
QDEDATSENN ANKKVSESLF EDEDDIFGDK PVASKKSSNS YTSSLSDILG GDDEDDLFGG
SKKKQQQQED ADGDEPTKKK DPFADELNNT LSSKNKGGDD DLFGGSSSTT TTSKPKKKSM
FDDDLFGDSE ETPAPKSSAS RKVTFDDSLF GDDETALPTS KKDSTTTSSQ PQQKKTLNDL
FTDEDLTPAT KKETKKSSSL SFLDGMDEDD LFGTPKPKST TTSAAPTATT TKPPTPIPDS
DASGSESTTG KSSPAPKKPV GGVKLFDFDA AGGDIFSGKK VGTGGKSSPS PAPKTESKAS
EDDFFSSDKK STSATKKDAE IFGSEGGLLD VPEKKIKRHD PKKYVDSDVL GIDSISSDKN
KPKVQDPFGV GAAGSNDDDS FDIPKANSKK EQETKPTKST TTDDDLDIPK APTTATTTTT
TKPAVKKSTK SNFFDFEDTD SPLSSNKKSG GDDDLFSFDS SKTTTETKKQ PITEEPKKKQ
DTTTTPSIDP FSGLDIKKPS EASISPASPV STIESDPLFG DIKKPVAQKP KKTNFFDGDL
TKDEPAKSEP TKVEPTKVEP TKAEPTKVEP AKVEPTKVES DKKESNPLGG GDEESVFGDF
SIKKNPTTSS STTATENKPA VKKSTKSNFF DFEDTDSPLS SNKKNDDDIF SSTTKKSTTT
TTTTTSSKDF FGDLDTSSGN KKVEEKKSSD FDSFFSGSDD PLGITKPKSK TTTTPPLTST
TANIESDDPL SLGLPTFTNN NNNNNAKPPP PPSPLGEDVP SPLTSNNTKN RTKSIGSMFE
AQQQLQQQQQ EKNRSESPTS EKAEPTKKTS NISSLQNKLS LNPNMFLPGS KMPKKKKEES
ENEDDSSTNN DNDSSATDLS DSGRSSPSVT SPTLSGGRPR RNTVSSRNNN DSEESEPVKE
LTHATASRPK SGGRRPPTRK SGTSAPNRSE SPTPTQKSDS ESEKVRSSSP ITTKPVVSEP
TKKTISNTSF FDDIPSEKTS SGKSSPSPTI KSTSEKVTID PLGDIFASTT TKPTASETKA
ALSSAPKKSE PETPKETPKE TPKEKEQTKE KEQPKETPKT TTTKKSTKAV ETKSETDSFF
DDIPDIISRP KASVKSAAAK KASSTTKPVI SDDIFDDITS TTSKSTTTTT TTTTTKAKST
KAVDNLFDDD TNTTTKATPT KATPSKSKPK AKNVENLFD
