WASC3_MOUSE
ID WASC3_MOUSE Reviewed; 194 AA.
AC Q9CR27; Q8K2X5; Q9D0Y7;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=WASH complex subunit 3 {ECO:0000312|MGI:MGI:1914532};
DE AltName: Full=Coiled-coil domain-containing protein 53;
GN Name=Washc3 {ECO:0000312|MGI:MGI:1914532}; Synonyms=Ccdc53;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Hippocampus, Tongue, and Visual cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts as a component of the WASH core complex that functions
CC as a nucleation-promoting factor (NPF) at the surface of endosomes,
CC where it recruits and activates the Arp2/3 complex to induce actin
CC polymerization, playing a key role in the fission of tubules that serve
CC as transport intermediates during endosome sortingg.
CC {ECO:0000250|UniProtKB:Q9Y3C0}.
CC -!- SUBUNIT: Component of the WASH core complex also described as WASH
CC regulatory complex (SHRC) composed of WASHC1, WASHC2, WASHC3, WASHC4
CC and WASHC5. The WASH core complex associates via WASHC2 with the F-
CC actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and
CC CAPZB) in a transient or substoichiometric manner which was initially
CC described as WASH complex. {ECO:0000250|UniProtKB:Q9Y3C0}.
CC -!- SUBCELLULAR LOCATION: Early endosome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CCDC53 family. {ECO:0000305}.
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DR EMBL; AK004206; BAB23220.1; -; mRNA.
DR EMBL; AK009358; BAB26239.1; -; mRNA.
DR EMBL; AK013835; BAB29011.1; -; mRNA.
DR EMBL; AK158822; BAE34682.1; -; mRNA.
DR EMBL; BC029256; AAH29256.1; -; mRNA.
DR CCDS; CCDS24108.1; -.
DR RefSeq; NP_001116432.1; NM_001122960.1.
DR RefSeq; NP_080346.1; NM_026070.3.
DR RefSeq; NP_081763.1; NM_027487.1.
DR AlphaFoldDB; Q9CR27; -.
DR SMR; Q9CR27; -.
DR BioGRID; 212071; 1.
DR ComplexPortal; CPX-1177; WASH complex, variant WASHC1/WASHC2.
DR IntAct; Q9CR27; 2.
DR MINT; Q9CR27; -.
DR STRING; 10090.ENSMUSP00000020248; -.
DR iPTMnet; Q9CR27; -.
DR PhosphoSitePlus; Q9CR27; -.
DR EPD; Q9CR27; -.
DR MaxQB; Q9CR27; -.
DR PaxDb; Q9CR27; -.
DR PeptideAtlas; Q9CR27; -.
DR PRIDE; Q9CR27; -.
DR ProteomicsDB; 297841; -.
DR Antibodypedia; 49055; 52 antibodies from 15 providers.
DR DNASU; 67282; -.
DR Ensembl; ENSMUST00000020248; ENSMUSP00000020248; ENSMUSG00000020056.
DR GeneID; 67282; -.
DR KEGG; mmu:67282; -.
DR UCSC; uc007grh.2; mouse.
DR CTD; 51019; -.
DR MGI; MGI:1914532; Washc3.
DR VEuPathDB; HostDB:ENSMUSG00000020056; -.
DR eggNOG; KOG4496; Eukaryota.
DR GeneTree; ENSGT00390000014084; -.
DR InParanoid; Q9CR27; -.
DR OMA; GCETKFV; -.
DR OrthoDB; 1425330at2759; -.
DR PhylomeDB; Q9CR27; -.
DR TreeFam; TF318955; -.
DR BioGRID-ORCS; 67282; 2 hits in 69 CRISPR screens.
DR ChiTaRS; Ccdc53; mouse.
DR PRO; PR:Q9CR27; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q9CR27; protein.
DR Bgee; ENSMUSG00000020056; Expressed in lens of camera-type eye and 272 other tissues.
DR ExpressionAtlas; Q9CR27; baseline and differential.
DR Genevisible; Q9CR27; MM.
DR GO; GO:0031901; C:early endosome membrane; IC:ComplexPortal.
DR GO; GO:0071203; C:WASH complex; IDA:MGI.
DR GO; GO:0030041; P:actin filament polymerization; IBA:GO_Central.
DR GO; GO:0016197; P:endosomal transport; IC:ComplexPortal.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0034315; P:regulation of Arp2/3 complex-mediated actin nucleation; IC:ComplexPortal.
DR InterPro; IPR019309; WASHC3.
DR PANTHER; PTHR13015; PTHR13015; 1.
DR Pfam; PF10152; CCDC53; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Endosome; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..194
FT /note="WASH complex subunit 3"
FT /id="PRO_0000076202"
FT REGION 94..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 158..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 46..74
FT /evidence="ECO:0000255"
FT COMPBIAS 94..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y3C0"
FT CONFLICT 140
FT /note="L -> R (in Ref. 2; AAH29256)"
FT /evidence="ECO:0000305"
FT CONFLICT 153
FT /note="R -> G (in Ref. 1; BAB23220)"
FT /evidence="ECO:0000305"
FT CONFLICT 179
FT /note="E -> K (in Ref. 2; AAH29256)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 194 AA; 21093 MW; 2BC096A01AFE566E CRC64;
MDEDGLPLMG SGIDLTKVPA IQQKRTVAFL NQFVVHTVQF LNRFSAVCEE KLADLSLRIQ
QIETTLNILD AKLSSIPGLE DVTVEVSPLN VTAVTNGSHS ETTSEQTQQN STQDSGAQES
EAPSENVLTV AKDPRYARYL KMVQVGVPVM AIRDKMISEG LDPELLEKPD APVPNGESER
AVEESSDSDS SFSD
