ID   WASC4_DICDI             Reviewed;        1135 AA.
AC   Q54R74;
DT   16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=WASH complex subunit 4 {ECO:0000250|UniProtKB:Q2M389};
DE   AltName: Full=WASH complex subunit SWIP homolog;
GN   Name=washc4 {ECO:0000250|UniProtKB:Q2M389}; ORFNames=DDB_G0283355;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-35; 286-296; 378-389; 425-435; 475-483; 814-828 AND
RP   993-1003, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=AX2;
RA   Bienvenut W.V., Sumpton D., Ura S., Insall R.H.;
RL   Submitted (OCT-2008) to UniProtKB.
CC   -!- SUBUNIT: Probable component of the WASH complex.
CC       {ECO:0000250|UniProtKB:Q2M389}.
CC   -!- SIMILARITY: Belongs to the SWIP family. {ECO:0000305}.
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DR   EMBL; AAFI02000054; EAL65754.1; -; Genomic_DNA.
DR   RefSeq; XP_639109.1; XM_634017.1.
DR   AlphaFoldDB; Q54R74; -.
DR   SMR; Q54R74; -.
DR   STRING; 44689.DDB0234041; -.
DR   PaxDb; Q54R74; -.
DR   EnsemblProtists; EAL65754; EAL65754; DDB_G0283355.
DR   GeneID; 8624042; -.
DR   KEGG; ddi:DDB_G0283355; -.
DR   dictyBase; DDB_G0283355; swip.
DR   eggNOG; KOG3578; Eukaryota.
DR   HOGENOM; CLU_002451_0_0_1; -.
DR   InParanoid; Q54R74; -.
DR   OMA; QFLFDEH; -.
DR   PhylomeDB; Q54R74; -.
DR   PRO; PR:Q54R74; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0071203; C:WASH complex; IDA:dictyBase.
DR   GO; GO:0030041; P:actin filament polymerization; IMP:dictyBase.
DR   GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR   GO; GO:0007032; P:endosome organization; IBA:GO_Central.
DR   GO; GO:0006887; P:exocytosis; IMP:dictyBase.
DR   GO; GO:0044655; P:phagosome reneutralization; IMP:dictyBase.
DR   GO; GO:0061462; P:protein localization to lysosome; IMP:dictyBase.
DR   InterPro; IPR028191; WASH-4_N.
DR   InterPro; IPR028283; WASH-7_C.
DR   InterPro; IPR028282; WASH-7_central.
DR   InterPro; IPR027307; WASH7.
DR   PANTHER; PTHR31409; PTHR31409; 1.
DR   Pfam; PF14746; WASH-7_C; 1.
DR   Pfam; PF14744; WASH-7_mid; 1.
DR   Pfam; PF14745; WASH-7_N; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Direct protein sequencing; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|Ref.2"
FT   CHAIN           2..1135
FT                   /note="WASH complex subunit 4"
FT                   /id="PRO_0000356843"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000269|Ref.2"
SQ   SEQUENCE   1135 AA;  131571 MW;  A90C3A1043280A84 CRC64;
     MATSYSANNG QNEEYTFDAF EDGSSKLVGE QQLTKLNQFA VDYAQQIWKI EEALDETLNE
     VWDINIDPVS IYNKPHEQLD LIELVKTDHK IFNKVILVFS SICNQTRILK ETAESKFYSP
     LTVFGEITGE SSEGDVQIEV GKLLPFMIDL SAFVNRCYSL IRNIISQFAS IYQSQKNIHT
     QFFKNVHLQA VYYSMIDIFS VLINLDSIIT QNTALDSSWG RYLRMVKSVK QEPNKYSVSG
     EEDKLWQLEK LLLSLKGQLL EGFIFQSCIQ QEFDFPGVID VKGNKVLKAE FQYNVKVLWS
     MFGTKIMDSS ELNLRERFPG FMGLYAFYIA LFKDITDKSF FKQVWEVTKR VPMVSINVNV
     FWFPADFIQQ LMPGMIKIVG STFNHMEIRR DYLRNVDKEF SGRVKSYYLQ VSRWMVRMES
     SQTRGGTLWD VSLSKVGQII QGVQLSYHIS HLLKTMIGLH MHLTAPLKSS DVRKLFQCAE
     MLKSIENTFH RRSAMISAHI SMMVQQLTDI INEKLNVVRS KYAGRSNYSE IELDVIVALS
     LCSDLLCGVA TNERITVVRL CLNVIYQSNI LKENDIEELR LHIKRLEFIS DIGKIVKASC
     DCSILFWSRD LFPTYLQFLY QNPSQATSLQ YTLTGLKDVV SVLDKAIHVD NAKQRLIDVY
     RNEMEEMIDK NIIQPLGKDV ETDLRLHIHA FLNIEEKDPF KTGIKEFGKF LELKPLRFFD
     RTIDIKSRIS HYLDQTFYNL NTVALFDWKT YSEMRNMAFY KYGLQLLEVH LPGSTLEQGL
     DVLEIMRNIH IFVSRYNYNL NNQIFIQRSS NSKTLNTINI THISNSIRTH GSGIMNTTIN
     FAYRFLVQKF SIFSEFLFDD QIKSKLYKNI KYFRENKEQL NNMYPSELVT ELERDIRQLG
     VSETGLTFLD HFRLLITHIG NAMGYIRLVR SGGLHYCSNA IKFVPDLKKI PKFQDLTSKD
     ALSPETIQAS TNLDSIIHNL SNNLSEGTEY FKMLVNVFAT EFRNIANQHL KNFYIIVPAL
     ASNYIDHMIN SKDKLFKKSK AIGAEALLFT DDGFAIGLAY ILKLLDQNKD FDSLHWFDRI
     QLKCETDQKR MLAEANAKGI KEDQNHLAIK KIQNFQQEYE LFKYSFSGSR IFFKD