WASC4_DROME
ID WASC4_DROME Reviewed; 1102 AA.
AC Q9VXH9;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 14-MAY-2014, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=WASH complex subunit 4 {ECO:0000250|UniProtKB:Q2M389};
DE AltName: Full=Strumpellin and WASH-interacting protein homolog {ECO:0000305};
DE AltName: Full=WASH complex subunit SWIP homolog;
GN Name=SWIP {ECO:0000312|FlyBase:FBgn0030739};
GN ORFNames=CG13957 {ECO:0000312|FlyBase:FBgn0030739};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP SUBUNIT.
RX PubMed=20498093; DOI=10.1073/pnas.0913293107;
RA Jia D., Gomez T.S., Metlagel Z., Umetani J., Otwinowski Z., Rosen M.K.,
RA Billadeau D.D.;
RT "WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein
RT (WASP) family are controlled by analogous structurally related complexes.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:10442-10447(2010).
RN [4]
RP FUNCTION.
RX PubMed=25739458; DOI=10.1091/mbc.e14-08-1266;
RA Verboon J.M., Rahe T.K., Rodriguez-Mesa E., Parkhurst S.M.;
RT "Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune
RT cell developmental migrations.";
RL Mol. Biol. Cell 26:1665-1674(2015).
CC -!- FUNCTION: Acts at least in part as component of the WASH complex which
CC may regulate wash nucleation-promoting factor (NPF) activity and is
CC required for its membrane targeting during endosomal sorting (By
CC similarity). During embryogenesis, not involved in the wash-dependent
CC developmental migration of hemocytes anteriorly from the tail
CC (PubMed:25739458). {ECO:0000250|UniProtKB:Q2M389,
CC ECO:0000269|PubMed:25739458}.
CC -!- SUBUNIT: Component of the WASH complex. {ECO:0000269|PubMed:20498093}.
CC -!- SUBCELLULAR LOCATION: Early endosome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the SWIP family. {ECO:0000305}.
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DR EMBL; AE014298; AAF48588.2; -; Genomic_DNA.
DR RefSeq; NP_573116.2; NM_132888.2.
DR AlphaFoldDB; Q9VXH9; -.
DR BioGRID; 58934; 4.
DR IntAct; Q9VXH9; 7.
DR STRING; 7227.FBpp0074003; -.
DR PaxDb; Q9VXH9; -.
DR PRIDE; Q9VXH9; -.
DR EnsemblMetazoa; FBtr0336975; FBpp0307917; FBgn0030739.
DR GeneID; 32594; -.
DR KEGG; dme:Dmel_CG13957; -.
DR UCSC; CG13957-RA; d. melanogaster.
DR CTD; 32594; -.
DR FlyBase; FBgn0030739; SWIP.
DR VEuPathDB; VectorBase:FBgn0030739; -.
DR eggNOG; KOG3578; Eukaryota.
DR GeneTree; ENSGT00390000002524; -.
DR HOGENOM; CLU_002451_0_0_1; -.
DR InParanoid; Q9VXH9; -.
DR OMA; PFLCNPH; -.
DR OrthoDB; 186054at2759; -.
DR BioGRID-ORCS; 32594; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32594; -.
DR PRO; PR:Q9VXH9; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030739; Expressed in eye disc (Drosophila) and 20 other tissues.
DR Genevisible; Q9VXH9; DM.
DR GO; GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IDA:FlyBase.
DR GO; GO:0071203; C:WASH complex; IDA:UniProtKB.
DR GO; GO:0016197; P:endosomal transport; ISS:FlyBase.
DR GO; GO:0007032; P:endosome organization; ISS:FlyBase.
DR GO; GO:0140591; P:nuclear envelope budding; IMP:FlyBase.
DR GO; GO:0045785; P:positive regulation of cell adhesion; IMP:FlyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR028191; WASH-4_N.
DR InterPro; IPR028283; WASH-7_C.
DR InterPro; IPR028282; WASH-7_central.
DR InterPro; IPR027307; WASH7.
DR PANTHER; PTHR31409; PTHR31409; 1.
DR Pfam; PF14746; WASH-7_C; 1.
DR Pfam; PF14744; WASH-7_mid; 1.
DR Pfam; PF14745; WASH-7_N; 1.
PE 1: Evidence at protein level;
KW Endosome; Protein transport; Reference proteome; Transport.
FT CHAIN 1..1102
FT /note="WASH complex subunit 4"
FT /id="PRO_0000390960"
SQ SEQUENCE 1102 AA; 127348 MW; 092B08EB45569E75 CRC64;
MFAEDILRDY GSFISEHDGK LKLLRDKVPN RDVSESAQCV YMSPVVEVNF MGNDSMAPDR
SFEESELVAN KPLTTLANLC NQCRNLSRKA KRFQLAFLFS DFRLDDTLPP HTHTSEGSAG
LEGSLVRMSS SMDFFCQVYF LLNRMIVILQ NLWRQIAASV SVPMDINEVH IFAVFDAMSE
LLEHIVVFNE LANQSKISTM WALYKKWLMN LSNSQSANLE LNGLSTSLMD IENLITKDFF
RILLDNLMEL KKQFGLNSVS PITQHSNAYI RRQLLDVDAN PSNELKNYED PKHIIRLTTF
VVVVHELGIQ MEGKLVKNVI DLVARHKQVP LNRSVFWSPS GFLSLHAKTL MKSSARSQDG
QGPKVHSTVL EKFRLSDQRT CRQLGVQLSL WSIQMQRVFD VGVFGHLKTF LQLILNGHSY
ADQVNLLAVA LINRHVALMT PMTRNDWIVV SRLLQYLKVI QKTFESNQIN FVRFISSLIQ
WQKQKVIHLL HTTKKKIVVL KLLQRKINFL ATIKLAEKSI MGFPSKQRLT FVNLALGEFL
DNRLLPADNQ KLIKSILHRV NSISDIMRNI GGQLNTSESS SLVYNHWFLD TSVLKEYTEL
QRNPYSLQNL VSVSHHLDKI MAMFRGSRCP KQSANDLIIE FLSNHLEFFL RVEALSHLFQ
SQDQPFQQSA LDYRLCINAV AVENDGDYNI IKDHLENYFT ATFYNLTTIA PHDWKSYEKM
RHLANKVLQL QPIDDHLPNQ IIDQGIDVLQ IMRNIHTFAS SYAYNMNLQV FVETNSRSKH
LDIIGTRHVA NSVQTHGTGI INTTVNFIYQ FLRQKFYTFS TFLHDEQIKS RLLKELRFHT
EHKHSKSYQS YPYERADSFL KKIRRLGCSS NGETYMDLFR KVITQVGNAV GYVRLLQAGS
KNANFRNRSY KTRFDSNFSC GGSKVHEATE GSIREYEKSL GHMKECYSDS TNYFKLLLQG
FQPFLCNPHN HHLRTFYLIT PALIMNYIDY RVKQKLKIYK KDQTKISLFE DGFAIGLVYI
LNMLNQQTEF HELGWSQTIT QHLNAERSKV RDILAGQQRT APEQLDEKLH QTVAITERHV
NAYEHEYNLL YATLSSSEIF FQ
