WASC5_DROME
ID WASC5_DROME Reviewed; 1191 AA.
AC Q9VUY8; A0AVW2; D3DMJ6;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=WASH complex subunit homolog 5 {ECO:0000250|UniProtKB:Q12768};
DE AltName: Full=WASH complex subunit strumpellin homolog {ECO:0000305};
GN Name=Strump {ECO:0000312|FlyBase:FBgn0036571};
GN ORFNames=CG12272 {ECO:0000312|FlyBase:FBgn0036571};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J., Frise E., Kapadia B., Park S., Wan K., Yu C.,
RA Celniker S., Booth B.;
RL Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBUNIT.
RX PubMed=20498093; DOI=10.1073/pnas.0913293107;
RA Jia D., Gomez T.S., Metlagel Z., Umetani J., Otwinowski Z., Rosen M.K.,
RA Billadeau D.D.;
RT "WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein
RT (WASP) family are controlled by analogous structurally related complexes.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:10442-10447(2010).
RN [5]
RP FUNCTION.
RX PubMed=25739458; DOI=10.1091/mbc.e14-08-1266;
RA Verboon J.M., Rahe T.K., Rodriguez-Mesa E., Parkhurst S.M.;
RT "Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune
RT cell developmental migrations.";
RL Mol. Biol. Cell 26:1665-1674(2015).
CC -!- FUNCTION: Acts at least in part as component of the WASH complex which
CC may regulate wash nucleation-promoting factor (NPF) activity and is
CC required for its membrane targeting during endosomal sorting (By
CC similarity). During embryogenesis, not involved in the wash-dependent
CC developmental migration of hemocytes anteriorly from the tail
CC (PubMed:25739458). {ECO:0000250|UniProtKB:Q6DIP9,
CC ECO:0000269|PubMed:25739458}.
CC -!- SUBUNIT: Component of the WASH complex. {ECO:0000269|PubMed:20498093}.
CC -!- SUBCELLULAR LOCATION: Early endosome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the strumpellin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF49534.1; -; Genomic_DNA.
DR EMBL; AE014296; ACZ94725.1; -; Genomic_DNA.
DR EMBL; BT029280; ABK30917.1; -; mRNA.
DR EMBL; BT120162; ADB91410.1; -; mRNA.
DR RefSeq; NP_001163454.1; NM_001169983.2.
DR RefSeq; NP_648840.1; NM_140583.4.
DR AlphaFoldDB; Q9VUY8; -.
DR BioGRID; 65075; 10.
DR IntAct; Q9VUY8; 1.
DR STRING; 7227.FBpp0075208; -.
DR PaxDb; Q9VUY8; -.
DR PRIDE; Q9VUY8; -.
DR DNASU; 39766; -.
DR EnsemblMetazoa; FBtr0075452; FBpp0075208; FBgn0036571.
DR EnsemblMetazoa; FBtr0301912; FBpp0291126; FBgn0036571.
DR GeneID; 39766; -.
DR KEGG; dme:Dmel_CG12272; -.
DR UCSC; CG12272-RA; d. melanogaster.
DR CTD; 39766; -.
DR FlyBase; FBgn0036571; Strump.
DR VEuPathDB; VectorBase:FBgn0036571; -.
DR eggNOG; KOG3666; Eukaryota.
DR GeneTree; ENSGT00390000011137; -.
DR HOGENOM; CLU_004021_1_0_1; -.
DR InParanoid; Q9VUY8; -.
DR OMA; FQSIHHY; -.
DR OrthoDB; 88928at2759; -.
DR PhylomeDB; Q9VUY8; -.
DR SignaLink; Q9VUY8; -.
DR BioGRID-ORCS; 39766; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Strumpellin; fly.
DR GenomeRNAi; 39766; -.
DR PRO; PR:Q9VUY8; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036571; Expressed in embryonic/larval hemocyte (Drosophila) and 27 other tissues.
DR ExpressionAtlas; Q9VUY8; baseline and differential.
DR Genevisible; Q9VUY8; DM.
DR GO; GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IDA:FlyBase.
DR GO; GO:0071203; C:WASH complex; IDA:UniProtKB.
DR GO; GO:0030041; P:actin filament polymerization; IBA:GO_Central.
DR GO; GO:0140285; P:endosome fission; IBA:GO_Central.
DR GO; GO:0007032; P:endosome organization; IBA:GO_Central.
DR GO; GO:0140591; P:nuclear envelope budding; IMP:FlyBase.
DR GO; GO:0045785; P:positive regulation of cell adhesion; IMP:FlyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0051125; P:regulation of actin nucleation; IBA:GO_Central.
DR InterPro; IPR019393; WASH_strumpellin.
DR PANTHER; PTHR15691; PTHR15691; 1.
DR Pfam; PF10266; Strumpellin; 1.
PE 1: Evidence at protein level;
KW Endosome; Protein transport; Reference proteome; Transport.
FT CHAIN 1..1191
FT /note="WASH complex subunit homolog 5"
FT /id="PRO_0000390953"
FT CONFLICT 1120
FT /note="V -> F (in Ref. 3; ABK30917)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1191 AA; 137274 MW; 118C8F30F1FD4AF4 CRC64;
MSFLDDNNAC GQNLLNIVSV GNSIIAEILR LKDYVPSIYR LDNKADKAKY GELILDFSYF
KIAEDHERRI EQSPELTELD DEARAQLPLI TRFYLAFQSI HHYASDLQQY IEELNTGYYI
QQTLETVLQE EEGRQLLCES LYLFGVILLM VDFHIPGDVR ERLLIAYYRY SGGDATPSGD
ESNIHDVCLL LRSTGYVHPS IAAKVLGLGG KQAGARAASL VVPRYPEAYF SRFRFDENFV
DLVVARLRCD DIYNQLNLYP HPAHRSTALS TQAAMLYVCL YFCPQVLHSQ GSQMREIVDK
FFCDNWTISV YMGMTVNLVD AWLDFKAARS AIENVISPPA IKALCQQQKE QLGKITQKTQ
EIVREGVLND NFVLEHANKI IHLMRQSNVL LRWFCLHTSR EVFIFAHTAT LTGQVQKCVL
HELQFNRNTL YNLLLNCSQM ELSVREFLAE IQQTKEERWT KSREEAMQRL NELSEAFAGS
RPLSKIEQNP QLQQWFGEVA GRLQKLELSR PQKSGRLIIQ VMQALDDVQE YHNLHSNMLV
KQQLQETRDM LNQMAQLINL KEDIEIHIQM ITDFSYAWHL LQFDFTPPMQ EHIKRQPQAV
IGIRAVFLKL ASTLEVPLMR INQARSEDLV SVSNYYSTEL ANFLRRVLQI VPETMFSILA
KIIYLLTNVI KEFPTKVEKE RLKDYAQFEE RAKVAQLTNS IAVFTKGILM MKTTLVGVIE
LDPKQLLEDG IRKELVNHLA NAYNLGLIFT PEKGKTPVQL LQQKLQALAK TIEGYRRSFE
YIEDYLRVQG LRILLEESQR IINYNVEKEC NAFLRNKVQE FQSEHQSQII PIPNFPPLLG
DPSNNFIGRL AHEILRCTDP KQTIFLDLKS TWYEKKAPHQ EVLAGSGFFE ILREALAPAG
MVGLERLYAH MLADELKRNL ERLQRNLTSD RMWVDTLAAL TRELEARDFP TPEVSKQPLK
YYQAYTQRWL KVWPTLLDWV LCIGQKQLLR REIAGELSFS SKCDAKLLEN TADTLNKALL
LELSLSKDLC DEKGVVMLTE LQETLLYTGN FEPLEQVFLI TKNTHNMALF MFLFTIAHLG
RMQHSTITDC LLPKSAKDNI DNVPFIVGLV TILQQFHKNV KMLYISYMSQ YVVTVSEAQL
LDKEILGPEV VTALHFLLAF IRIARLPLGV LEQRIPNIIL SEYEYLSTLL K
