WASH1_BOVIN
ID WASH1_BOVIN Reviewed; 471 AA.
AC A7Z063;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=WASH complex subunit 1 {ECO:0000250|UniProtKB:A8K0Z3};
DE AltName: Full=WAS protein family homolog 1;
GN Name=WASHC1 {ECO:0000250|UniProtKB:A8K0Z3}; Synonyms=WASH1;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Ascending colon;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP IDENTIFICATION IN THE WASH COMPLEX.
RX PubMed=20498093; DOI=10.1073/pnas.0913293107;
RA Jia D., Gomez T.S., Metlagel Z., Umetani J., Otwinowski Z., Rosen M.K.,
RA Billadeau D.D.;
RT "WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein
RT (WASP) family are controlled by analogous structurally related complexes.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:10442-10447(2010).
CC -!- FUNCTION: Acts as a component of the WASH core complex that functions
CC as a nucleation-promoting factor (NPF) at the surface of endosomes,
CC where it recruits and activates the Arp2/3 complex to induce actin
CC polymerization, playing a key role in the fission of tubules that serve
CC as transport intermediates during endosome sorting. Involved in
CC endocytic trafficking of EGF. Involved in transferrin receptor
CC recycling. Regulates the trafficking of endosomal alpha5beta1 integrin
CC to the plasma membrane and involved in invasive cell migration. In T-
CC cells involved in endosome-to-membrane recycling of receptors including
CC T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T-
CC cell proliferation and effector function. In dendritic cells involved
CC in endosome-to-membrane recycling of major histocompatibility complex
CC (MHC) class II probably involving retromer and subsequently allowing
CC antigen sampling, loading and presentation during T-cell activation.
CC Involved in negative regulation of autophagy independently from its
CC role in endosomal sorting by inhibiting BECN1 ubiquitination to
CC inactivate PIK3C3/Vps34 activity (By similarity).
CC {ECO:0000250|UniProtKB:A8K0Z3, ECO:0000250|UniProtKB:C4AMC7,
CC ECO:0000250|UniProtKB:Q8VDD8}.
CC -!- SUBUNIT: Component of the WASH core complex also described as WASH
CC regulatory complex SHRC composed of WASHC1, WASHC2, WASHC3, WASHC4 and
CC WASHC5. The WASH core complex associates with the F-actin-capping
CC protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB); the
CC assembly has been initially described as WASH complex
CC (PubMed:20498093). Interacts (via WHD1 region) with WASHC2; the
CC interaction is direct. Interacts with alpha-tubulin. Interacts with
CC BECN1; WASHC1 and AMBRA1 can competitively interact with BECN1.
CC Interacts with BLOC1S2; may associate with the BLOC-1 complex.
CC Interacts with tubulin gamma chain (TUBG1 or TUBG2) (By similarity).
CC Interacts with TBC1D23 (By similarity). {ECO:0000250|UniProtKB:A8K0Z3,
CC ECO:0000250|UniProtKB:C4AMC7, ECO:0000250|UniProtKB:Q8VDD8}.
CC -!- SUBCELLULAR LOCATION: Early endosome membrane
CC {ECO:0000250|UniProtKB:A8K0Z3}. Recycling endosome membrane
CC {ECO:0000250|UniProtKB:Q8VDD8}. Late endosome
CC {ECO:0000250|UniProtKB:A8K0Z3}. Cytoplasmic vesicle, autophagosome
CC {ECO:0000250|UniProtKB:Q8VDD8}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome, centriole
CC {ECO:0000250|UniProtKB:Q8VDD8}. Note=Localization to the endosome
CC membrane is mediated via its interaction with WASHC2.
CC {ECO:0000250|UniProtKB:A8K0Z3}.
CC -!- DOMAIN: The VCA (verprolin, cofilin, acidic) domain promotes actin
CC polymerization by the Arp2/3 complex in vitro.
CC {ECO:0000250|UniProtKB:C4AMC7}.
CC -!- SIMILARITY: Belongs to the WASH1 family. {ECO:0000305}.
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DR EMBL; BC153261; AAI53262.1; -; mRNA.
DR RefSeq; NP_001098876.1; NM_001105406.1.
DR AlphaFoldDB; A7Z063; -.
DR SMR; A7Z063; -.
DR STRING; 9913.ENSBTAP00000015790; -.
DR PaxDb; A7Z063; -.
DR PeptideAtlas; A7Z063; -.
DR PRIDE; A7Z063; -.
DR Ensembl; ENSBTAT00000069933; ENSBTAP00000059564; ENSBTAG00000011902.
DR GeneID; 533602; -.
DR KEGG; bta:533602; -.
DR CTD; 100287171; -.
DR VEuPathDB; HostDB:ENSBTAG00000011902; -.
DR eggNOG; ENOG502QSX3; Eukaryota.
DR GeneTree; ENSGT00390000016717; -.
DR HOGENOM; CLU_029156_1_0_1; -.
DR InParanoid; A7Z063; -.
DR OMA; MYSAAKY; -.
DR OrthoDB; 904881at2759; -.
DR TreeFam; TF318222; -.
DR Proteomes; UP000009136; Chromosome 5.
DR Bgee; ENSBTAG00000011902; Expressed in retina and 105 other tissues.
DR ExpressionAtlas; A7Z063; baseline and differential.
DR GO; GO:0005776; C:autophagosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0055037; C:recycling endosome; ISS:UniProtKB.
DR GO; GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071203; C:WASH complex; IDA:UniProtKB.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0043014; F:alpha-tubulin binding; ISS:UniProtKB.
DR GO; GO:0043015; F:gamma-tubulin binding; IBA:GO_Central.
DR GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB.
DR GO; GO:0032456; P:endocytic recycling; IBA:GO_Central.
DR GO; GO:0016197; P:endosomal transport; ISS:UniProtKB.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB.
DR InterPro; IPR028290; WASH1.
DR InterPro; IPR021854; WASH1_WAHD.
DR InterPro; IPR003124; WH2_dom.
DR PANTHER; PTHR23331; PTHR23331; 1.
DR Pfam; PF11945; WASH_WAHD; 1.
DR PROSITE; PS51082; WH2; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endosome;
KW Membrane; Protein transport; Reference proteome; Transport.
FT CHAIN 1..471
FT /note="WASH complex subunit 1"
FT /id="PRO_0000390961"
FT DOMAIN 365..387
FT /note="WH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00406"
FT REGION 1..167
FT /note="WHD1"
FT REGION 1..54
FT /note="Required for WASH complex assembly"
FT /evidence="ECO:0000250|UniProtKB:C4AMC7"
FT REGION 297..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 353..471
FT /note="VCA"
FT /evidence="ECO:0000250|UniProtKB:C4AMC7"
FT COMPBIAS 301..336
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 383..402
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 471 AA; 50773 MW; 15F8DDCF10B5816A CRC64;
MTPTGTQHSL AGQTYAVPLI QPDLRREEAI QQVADALQYL QKVSGDIFSR ISQRVELSRS
QLQAIGERVS LAQAKIEKIK GSKKAIKVFS SAKYPAPERL QEYGSIFMGA QDPGLQRRPR
HRIQSKHRPL DERALQEKLK FFPVCVNTKP EPEDEAEEGL GGLPSNISSV SSLLLFNTTE
NLYKKYVFLD PLAGAVTKTH VMLGAETEEK LFDAPLSISR REQLERQVPE NYFYVPDLGQ
VPDIDVPSYL PDLPGVADDL MYSADLGPGI APSAPGAIPE LPTFHTEVAQ PFKPDLEDGV
LTARPPPPPP PPPPPAPAVL MSVPPPPPPP QAPPGQPAKG DDSGGASPSA PVQGAPKEVV
DPSSGRATLL ESIRQAGGIG KAKLRSVKER KLEKKKQKEQ EQVRATSQGG DLMSDLFNKL
AMRRKGISGK GPGSGASEGP GGAFARMSDS IPPLPPPQQP PGEEDEDDWE S
