WAXS1_ARATH
ID WAXS1_ARATH Reviewed; 341 AA.
AC Q9FJ72;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Probable long-chain-alcohol O-fatty-acyltransferase 1;
DE EC=2.3.1.75;
DE AltName: Full=Wax synthase 1;
GN Name=AT1; OrderedLocusNames=At5g55380; ORFNames=MTE17.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION.
RX PubMed=10712527; DOI=10.1104/pp.122.3.645;
RA Lardizabal K.D., Metz J.G., Sakamoto T., Hutton W.C., Pollard M.R.,
RA Lassner M.W.;
RT "Purification of a jojoba embryo wax synthase, cloning of its cDNA, and
RT production of high levels of wax in seeds of transgenic arabidopsis.";
RL Plant Physiol. 122:645-655(2000).
CC -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC linear esters (waxes). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
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DR EMBL; AB015479; BAB08555.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96622.1; -; Genomic_DNA.
DR EMBL; AY090447; AAL91291.1; -; mRNA.
DR EMBL; AY143868; AAN28807.1; -; mRNA.
DR RefSeq; NP_200349.1; NM_124920.4.
DR AlphaFoldDB; Q9FJ72; -.
DR BioGRID; 20875; 12.
DR IntAct; Q9FJ72; 12.
DR STRING; 3702.AT5G55380.1; -.
DR PaxDb; Q9FJ72; -.
DR PRIDE; Q9FJ72; -.
DR ProteomicsDB; 242483; -.
DR EnsemblPlants; AT5G55380.1; AT5G55380.1; AT5G55380.
DR GeneID; 835631; -.
DR Gramene; AT5G55380.1; AT5G55380.1; AT5G55380.
DR KEGG; ath:AT5G55380; -.
DR Araport; AT5G55380; -.
DR TAIR; locus:2173962; AT5G55380.
DR eggNOG; ENOG502QSCR; Eukaryota.
DR HOGENOM; CLU_045902_0_0_1; -.
DR InParanoid; Q9FJ72; -.
DR OMA; NLIEVWI; -.
DR OrthoDB; 853289at2759; -.
DR PhylomeDB; Q9FJ72; -.
DR BioCyc; ARA:AT5G55380-MON; -.
DR BRENDA; 2.3.1.75; 399.
DR PRO; PR:Q9FJ72; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJ72; baseline and differential.
DR Genevisible; Q9FJ72; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR044851; Wax_synthase.
DR InterPro; IPR032805; Wax_synthase_dom.
DR InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR PANTHER; PTHR31595; PTHR31595; 1.
DR Pfam; PF13813; MBOAT_2; 1.
DR PIRSF; PIRSF037006; Wax_synthase; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..341
FT /note="Probable long-chain-alcohol O-fatty-acyltransferase
FT 1"
FT /id="PRO_0000380677"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 341 AA; 38734 MW; D5CECD02CAA66B94 CRC64;
MEEKFRNLIE VWISALISLS YCYYISSKLS KGVLRLLSIL PVCILFLVLP LFLSCVHFCA
ISVLFLSWLA NFKLLLFAFD EGPLFPLPPK LSRFICFACL PIKIRQDPSP NAIPNLHPKP
MPKWVLAVKI LVLGVLLHVY EYRDGLPRFV VLALYCLHIY LEVELVLVFV GAVVSTLLGC
NIEPVFNEPY LATSLQDFWS RRWNLMVSAV LRSTVHIPVQ RFFKRILSPD GAMFAGVMAS
FFVSGLMHEL LYFYMIRKPP TWEVTCFFVL HGAATATEIA VKRTQWLRPP HRAVSGLVVL
TFVSVTGVWL FLAQVLRNNV HEKAIGECLL VLDLAKLFTS S
