ID   WAXS2_ARATH             Reviewed;         343 AA.
AC   Q9FJ73;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Probable long-chain-alcohol O-fatty-acyltransferase 2;
DE            EC=2.3.1.75;
DE   AltName: Full=Wax synthase 2;
GN   Name=AT2; OrderedLocusNames=At5g55370; ORFNames=MTE17.8;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA   Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT   features of the regions of 1,013,767 bp covered by sixteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:297-308(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=10712527; DOI=10.1104/pp.122.3.645;
RA   Lardizabal K.D., Metz J.G., Sakamoto T., Hutton W.C., Pollard M.R.,
RA   Lassner M.W.;
RT   "Purification of a jojoba embryo wax synthase, cloning of its cDNA, and
RT   production of high levels of wax in seeds of transgenic arabidopsis.";
RL   Plant Physiol. 122:645-655(2000).
CC   -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC       linear esters (waxes). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC         CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
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DR   EMBL; AB015479; BAB08554.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96621.1; -; Genomic_DNA.
DR   RefSeq; NP_200348.1; NM_124919.2.
DR   AlphaFoldDB; Q9FJ73; -.
DR   STRING; 3702.AT5G55370.1; -.
DR   PaxDb; Q9FJ73; -.
DR   PRIDE; Q9FJ73; -.
DR   EnsemblPlants; AT5G55370.1; AT5G55370.1; AT5G55370.
DR   GeneID; 835630; -.
DR   Gramene; AT5G55370.1; AT5G55370.1; AT5G55370.
DR   KEGG; ath:AT5G55370; -.
DR   Araport; AT5G55370; -.
DR   TAIR; locus:2173947; AT5G55370.
DR   eggNOG; ENOG502SFJV; Eukaryota.
DR   HOGENOM; CLU_045902_0_0_1; -.
DR   InParanoid; Q9FJ73; -.
DR   OMA; LMHEAMM; -.
DR   OrthoDB; 853289at2759; -.
DR   PhylomeDB; Q9FJ73; -.
DR   BioCyc; ARA:AT5G55370-MON; -.
DR   BRENDA; 2.3.1.75; 399.
DR   PRO; PR:Q9FJ73; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FJ73; baseline and differential.
DR   Genevisible; Q9FJ73; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR044851; Wax_synthase.
DR   InterPro; IPR032805; Wax_synthase_dom.
DR   InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR   PANTHER; PTHR31595; PTHR31595; 1.
DR   Pfam; PF13813; MBOAT_2; 1.
DR   PIRSF; PIRSF037006; Wax_synthase; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..343
FT                   /note="Probable long-chain-alcohol O-fatty-acyltransferase
FT                   2"
FT                   /id="PRO_0000380678"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        235..255
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        292..312
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   343 AA;  39807 MW;  07A6F928CA09AD18 CRC64;
     MEEELRNLIK VWISALISIS YCYYISSKIS KGVLRLLSLL PIFIIFLLLP LFFSSVHFCV
     ISGFFFTWLA NFKLFLFAFD QEPLSPLPSN LTRFFCFACF PIKINKNPSS NRIHNKPMSK
     WVLAFKLLIF SFLLHVYRNN YDSGLSRFAF LALFTIHVYL EAELILVFVG ALMSMLLGCE
     MEPVFNDPYL ATSLQEFWSR RWNLMVPAVL RPAVHIPVQR FCAPLLGLHR AFYAGMLATF
     IVSGLMHELI YFYVIRKSPT WEVTCFFLLH GVVTCLEIAM KRMRWLPTPR RAVSGLAITV
     FLLVTAGWLF YPQMLRNDVH KRVISECLLV IDVVKRHVVC ILM