ID   WAXS3_ARATH             Reviewed;         342 AA.
AC   Q9FJ74;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Probable long-chain-alcohol O-fatty-acyltransferase 3;
DE            EC=2.3.1.75;
DE   AltName: Full=Wax synthase 3;
GN   Name=AT3; OrderedLocusNames=At5g55360; ORFNames=MTE17.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA   Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT   features of the regions of 1,013,767 bp covered by sixteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:297-308(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=10712527; DOI=10.1104/pp.122.3.645;
RA   Lardizabal K.D., Metz J.G., Sakamoto T., Hutton W.C., Pollard M.R.,
RA   Lassner M.W.;
RT   "Purification of a jojoba embryo wax synthase, cloning of its cDNA, and
RT   production of high levels of wax in seeds of transgenic arabidopsis.";
RL   Plant Physiol. 122:645-655(2000).
CC   -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC       linear esters (waxes). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC         CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
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DR   EMBL; AB015479; BAB08553.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96620.1; -; Genomic_DNA.
DR   RefSeq; NP_200347.1; NM_124918.2.
DR   AlphaFoldDB; Q9FJ74; -.
DR   STRING; 3702.AT5G55360.1; -.
DR   PaxDb; Q9FJ74; -.
DR   PRIDE; Q9FJ74; -.
DR   EnsemblPlants; AT5G55360.1; AT5G55360.1; AT5G55360.
DR   GeneID; 835629; -.
DR   Gramene; AT5G55360.1; AT5G55360.1; AT5G55360.
DR   KEGG; ath:AT5G55360; -.
DR   Araport; AT5G55360; -.
DR   TAIR; locus:2173932; AT5G55360.
DR   HOGENOM; CLU_045902_0_0_1; -.
DR   InParanoid; Q9FJ74; -.
DR   OMA; MRTDHAR; -.
DR   OrthoDB; 853289at2759; -.
DR   PhylomeDB; Q9FJ74; -.
DR   BioCyc; ARA:AT5G55360-MON; -.
DR   BRENDA; 2.3.1.75; 399.
DR   PRO; PR:Q9FJ74; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FJ74; baseline and differential.
DR   Genevisible; Q9FJ74; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR044851; Wax_synthase.
DR   InterPro; IPR032805; Wax_synthase_dom.
DR   InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR   PANTHER; PTHR31595; PTHR31595; 1.
DR   Pfam; PF13813; MBOAT_2; 1.
DR   PIRSF; PIRSF037006; Wax_synthase; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..342
FT                   /note="Probable long-chain-alcohol O-fatty-acyltransferase
FT                   3"
FT                   /id="PRO_0000380679"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        255..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   342 AA;  39408 MW;  A8183B8C95415445 CRC64;
     MEEELKNFIK LWISAIISIS YCYYLSTGIK AGVFRLLSVL PVCALFLVFP LFFSYVHFSG
     CMAFFLSWLA NFKLILFSFD QGPLSPLPRT LSRFICITCF PIKPQQNPNI QNYKIPIWLF
     AIKVVIFVVL LQMYEYKQYL SPALLLVFNS LHIFLELEIV FMLVKALVFI TLGCDLEPQS
     NEPYLATSLQ DFWGRRWNLM VPAILRPAVY LPARRMACRK VNSDQAMFLG VFAAFLVSGA
     VHEMLFFYLT REVPTGEVTW FFLLHGVCTV AEVAVKKSTF VRRWWRVSPT VSRLLTVGFV
     VVTSGWFFFP LIRSGIIERL ASEALMCIDF VKHKFLLLLL GD