ID   WAXS4_ARATH             Reviewed;         345 AA.
AC   Q9FJ75;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Probable long-chain-alcohol O-fatty-acyltransferase 4;
DE            EC=2.3.1.75;
DE   AltName: Full=Wax synthase 4;
GN   Name=AT4; OrderedLocusNames=At5g55350; ORFNames=MTE17.6;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA   Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT   features of the regions of 1,013,767 bp covered by sixteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:297-308(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=10712527; DOI=10.1104/pp.122.3.645;
RA   Lardizabal K.D., Metz J.G., Sakamoto T., Hutton W.C., Pollard M.R.,
RA   Lassner M.W.;
RT   "Purification of a jojoba embryo wax synthase, cloning of its cDNA, and
RT   production of high levels of wax in seeds of transgenic arabidopsis.";
RL   Plant Physiol. 122:645-655(2000).
CC   -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC       linear esters (waxes). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC         CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
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DR   EMBL; AB015479; BAB08552.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96619.1; -; Genomic_DNA.
DR   EMBL; DQ056722; AAY78866.1; -; mRNA.
DR   RefSeq; NP_200346.1; NM_124917.2.
DR   AlphaFoldDB; Q9FJ75; -.
DR   STRING; 3702.AT5G55350.1; -.
DR   PaxDb; Q9FJ75; -.
DR   PRIDE; Q9FJ75; -.
DR   EnsemblPlants; AT5G55350.1; AT5G55350.1; AT5G55350.
DR   GeneID; 835628; -.
DR   Gramene; AT5G55350.1; AT5G55350.1; AT5G55350.
DR   KEGG; ath:AT5G55350; -.
DR   Araport; AT5G55350; -.
DR   TAIR; locus:2173917; AT5G55350.
DR   eggNOG; ENOG502QSCR; Eukaryota.
DR   HOGENOM; CLU_045902_0_0_1; -.
DR   InParanoid; Q9FJ75; -.
DR   OMA; YYINREM; -.
DR   OrthoDB; 853289at2759; -.
DR   PhylomeDB; Q9FJ75; -.
DR   BioCyc; ARA:AT5G55350-MON; -.
DR   BRENDA; 2.3.1.75; 399.
DR   PRO; PR:Q9FJ75; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FJ75; baseline and differential.
DR   Genevisible; Q9FJ75; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR044851; Wax_synthase.
DR   InterPro; IPR032805; Wax_synthase_dom.
DR   InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR   PANTHER; PTHR31595; PTHR31595; 1.
DR   Pfam; PF13813; MBOAT_2; 1.
DR   PIRSF; PIRSF037006; Wax_synthase; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..345
FT                   /note="Probable long-chain-alcohol O-fatty-acyltransferase
FT                   4"
FT                   /id="PRO_0000380680"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        288..308
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   345 AA;  39825 MW;  F945759348905BFB CRC64;
     MEEELMSLIK VWVYAIISIS YCYYTSTRIK SGVFRLLSVL PVCVLFLVLP LFVSSVHFSG
     STAFFLSWLA NFKLILFSFD QGPLFPVPSN LSRFVCFTCF PIKLQQNPKP QNQMPKWGFA
     VKLAFFGVLL HMYEYKQHMS PTVLLVLYSL HIYLEYEILL APLKVLLSIS LWCDLEPHFN
     EPYLSTSLQD FWGRRWNLMV PAILRPAVYL PVRQMAGRKM NSDQALFLGV FASFLVSGVV
     HELIFFYFTR ESPTGEVTLF FVLHGVCTAA ECAAKRTRLV RRWKVSQMVS RLLTVGFVVM
     TGGWLFFPHL ARSGMIERLA DEAFLFIGFV KHKFFYLCRN QSLKS