WAXS5_ARATH
ID WAXS5_ARATH Reviewed; 333 AA.
AC Q9FJ76;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Probable long-chain-alcohol O-fatty-acyltransferase 5;
DE EC=2.3.1.75;
DE AltName: Full=Wax synthase 5;
GN Name=AT5; OrderedLocusNames=At5g55340; ORFNames=MTE17.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION.
RX PubMed=10712527; DOI=10.1104/pp.122.3.645;
RA Lardizabal K.D., Metz J.G., Sakamoto T., Hutton W.C., Pollard M.R.,
RA Lassner M.W.;
RT "Purification of a jojoba embryo wax synthase, cloning of its cDNA, and
RT production of high levels of wax in seeds of transgenic arabidopsis.";
RL Plant Physiol. 122:645-655(2000).
CC -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC linear esters (waxes). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
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DR EMBL; AB015479; BAB08551.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96618.1; -; Genomic_DNA.
DR EMBL; AY084724; AAM61298.1; -; mRNA.
DR RefSeq; NP_200345.1; NM_124916.2.
DR AlphaFoldDB; Q9FJ76; -.
DR PaxDb; Q9FJ76; -.
DR PRIDE; Q9FJ76; -.
DR EnsemblPlants; AT5G55340.1; AT5G55340.1; AT5G55340.
DR GeneID; 835627; -.
DR Gramene; AT5G55340.1; AT5G55340.1; AT5G55340.
DR KEGG; ath:AT5G55340; -.
DR Araport; AT5G55340; -.
DR TAIR; locus:2173902; AT5G55340.
DR HOGENOM; CLU_045902_0_0_1; -.
DR InParanoid; Q9FJ76; -.
DR OMA; MSSGWAL; -.
DR OrthoDB; 853289at2759; -.
DR PhylomeDB; Q9FJ76; -.
DR BioCyc; ARA:AT5G55340-MON; -.
DR BRENDA; 2.3.1.75; 399.
DR PRO; PR:Q9FJ76; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJ76; baseline and differential.
DR Genevisible; Q9FJ76; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR044851; Wax_synthase.
DR InterPro; IPR032805; Wax_synthase_dom.
DR InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR PANTHER; PTHR31595; PTHR31595; 1.
DR Pfam; PF13813; MBOAT_2; 1.
DR PIRSF; PIRSF037006; Wax_synthase; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..333
FT /note="Probable long-chain-alcohol O-fatty-acyltransferase
FT 5"
FT /id="PRO_0000380681"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 333 AA; 38036 MW; 2303634C33F886C4 CRC64;
MDEELKNLIK VWVSAIISIS YCYYIPPRIK SGAPRFLSVS PVLALFLVLP LFFSSLHLSL
ITAFFLTWLA NFKLILFSFD KGPLIPIPTN FPRFFCFTCF PIKVQQNPKS QNHLPKLVFA
IKLAIFAVLL HLYSYRQNLS PTILLGLYFV HLYLEIEIIL TFVKVVVFIS LGCDLEPQSN
KPYLATSLQD FWGRRWNLMV PAILRPAVYA PMRRVSERKM SSGWALFPGI LAAFIVSGLV
HELLFFYLIR EMPTGEVTLF FVLHGVCTAV ELAVKKKTTV AQRWRLSPGV SRVLTVGFVF
VTGGWLFTPQ LKRSGVMERF TSEAVLLVEF IKR
