WAXS6_ARATH
ID WAXS6_ARATH Reviewed; 346 AA.
AC Q9FJ77; A0MFP3;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Probable long-chain-alcohol O-fatty-acyltransferase 6;
DE EC=2.3.1.75;
DE AltName: Full=Wax synthase 6;
GN Name=AT6; OrderedLocusNames=At5g55330; ORFNames=MTE17.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP IDENTIFICATION.
RX PubMed=10712527; DOI=10.1104/pp.122.3.645;
RA Lardizabal K.D., Metz J.G., Sakamoto T., Hutton W.C., Pollard M.R.,
RA Lassner M.W.;
RT "Purification of a jojoba embryo wax synthase, cloning of its cDNA, and
RT production of high levels of wax in seeds of transgenic arabidopsis.";
RL Plant Physiol. 122:645-655(2000).
CC -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC linear esters (waxes). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28758.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB015479; BAB08550.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96617.1; -; Genomic_DNA.
DR EMBL; DQ447077; ABE66250.1; -; mRNA.
DR EMBL; DQ653369; ABK28758.1; ALT_SEQ; mRNA.
DR RefSeq; NP_200344.1; NM_124915.2.
DR AlphaFoldDB; Q9FJ77; -.
DR BioGRID; 20870; 19.
DR IntAct; Q9FJ77; 13.
DR STRING; 3702.AT5G55330.1; -.
DR PaxDb; Q9FJ77; -.
DR PRIDE; Q9FJ77; -.
DR EnsemblPlants; AT5G55330.1; AT5G55330.1; AT5G55330.
DR GeneID; 835626; -.
DR Gramene; AT5G55330.1; AT5G55330.1; AT5G55330.
DR KEGG; ath:AT5G55330; -.
DR Araport; AT5G55330; -.
DR TAIR; locus:2173887; AT5G55330.
DR HOGENOM; CLU_045902_0_0_1; -.
DR InParanoid; Q9FJ77; -.
DR OMA; FVTGVWL; -.
DR OrthoDB; 853289at2759; -.
DR PhylomeDB; Q9FJ77; -.
DR BioCyc; ARA:AT5G55330-MON; -.
DR BRENDA; 2.3.1.75; 399.
DR PRO; PR:Q9FJ77; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJ77; baseline and differential.
DR Genevisible; Q9FJ77; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR044851; Wax_synthase.
DR InterPro; IPR032805; Wax_synthase_dom.
DR InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR PANTHER; PTHR31595; PTHR31595; 1.
DR Pfam; PF13813; MBOAT_2; 1.
DR PIRSF; PIRSF037006; Wax_synthase; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..346
FT /note="Probable long-chain-alcohol O-fatty-acyltransferase
FT 6"
FT /id="PRO_0000380682"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 346 AA; 40395 MW; EDA3A7648C0081D6 CRC64;
MEEELKLFIQ VWVSAIISVT YCYYLTPKIK TSLLRLLSVL PVCVLFLIIP IFFSTVHSSF
TIAFFLSGLA VPKLILFALE KGPLFPLPPN LPHFVCFACF PIKLQKKPNP ENTNHFPKWV
FALKVFIFGA LLLQAYHYKQ FLSTNFLLGL YALHIYLELE ISLTLIKFLV SITLGCDLEP
QFNEPYLATS LHDFWGHRWN LMVSKILWLA VYNPIRQWRA KSSEWDRFFA IFATFLVSGV
AHEILYFYLT REKPTWEVTW FFVLHGFCMA AEVALKRKTK LVQRWPVNPA VSRLLTVGFV
FVTGVWLFSP QPIRHGLMER FINEDLFLID FFNRKLYILL GLFTSL
