ID   WAXS7_ARATH             Reviewed;         339 AA.
AC   Q9FJ78;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Probable long-chain-alcohol O-fatty-acyltransferase 7;
DE            EC=2.3.1.75;
DE   AltName: Full=Wax synthase 7;
GN   Name=AT7; OrderedLocusNames=At5g55320; ORFNames=MTE17.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA   Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT   features of the regions of 1,013,767 bp covered by sixteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:297-308(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=10712527; DOI=10.1104/pp.122.3.645;
RA   Lardizabal K.D., Metz J.G., Sakamoto T., Hutton W.C., Pollard M.R.,
RA   Lassner M.W.;
RT   "Purification of a jojoba embryo wax synthase, cloning of its cDNA, and
RT   production of high levels of wax in seeds of transgenic arabidopsis.";
RL   Plant Physiol. 122:645-655(2000).
CC   -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC       linear esters (waxes). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC         CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
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DR   EMBL; AB015479; BAB08549.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96616.1; -; Genomic_DNA.
DR   RefSeq; NP_200343.1; NM_124914.2.
DR   AlphaFoldDB; Q9FJ78; -.
DR   iPTMnet; Q9FJ78; -.
DR   PaxDb; Q9FJ78; -.
DR   PRIDE; Q9FJ78; -.
DR   EnsemblPlants; AT5G55320.1; AT5G55320.1; AT5G55320.
DR   GeneID; 835625; -.
DR   Gramene; AT5G55320.1; AT5G55320.1; AT5G55320.
DR   KEGG; ath:AT5G55320; -.
DR   Araport; AT5G55320; -.
DR   TAIR; locus:2173872; AT5G55320.
DR   eggNOG; ENOG502SGJ4; Eukaryota.
DR   HOGENOM; CLU_045902_0_0_1; -.
DR   InParanoid; Q9FJ78; -.
DR   OMA; ALPYYSY; -.
DR   OrthoDB; 853289at2759; -.
DR   PhylomeDB; Q9FJ78; -.
DR   BioCyc; ARA:AT5G55320-MON; -.
DR   BRENDA; 2.3.1.75; 399.
DR   PRO; PR:Q9FJ78; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FJ78; baseline and differential.
DR   Genevisible; Q9FJ78; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR044851; Wax_synthase.
DR   InterPro; IPR032805; Wax_synthase_dom.
DR   InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR   PANTHER; PTHR31595; PTHR31595; 1.
DR   Pfam; PF13813; MBOAT_2; 1.
DR   PIRSF; PIRSF037006; Wax_synthase; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..339
FT                   /note="Probable long-chain-alcohol O-fatty-acyltransferase
FT                   7"
FT                   /id="PRO_0000380683"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        287..307
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   339 AA;  38978 MW;  DFCBDA2FF3A07D9E CRC64;
     MEEEIKSLIN VGFLTIISVS YCYCLPPRIK SGVLRLLSIF PVCVLLVVLP LFFSFSIFTS
     TTAFFLSAIA NSRLILFSFD QGPLFPLPSN LFRFTCFTCF PIQRQQNPKS QDHLSTYVFP
     VKIAIFVVLL YVHNDIQNLP RTFLLCLHPL YVYLLLEILL TLLRILMTII LGCDLEPHFH
     EPYLATSLQD FWGRRWNLIV SASLRAIVYT PVRRVCQRVM SSDYAMLIGV FATFVTSGVA
     HEVVFFYITR AMPTGEVALF FLLHGVCTVA EVAAKRTAFV RRWPVRPVVS WMFTIAFVNV
     TAGWLFFPQL IRNNLGERCS NEISLLIDFF RSKLFYFPQ