WAXS8_ARATH
ID WAXS8_ARATH Reviewed; 336 AA.
AC Q9LNL1;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Probable long-chain-alcohol O-fatty-acyltransferase 8;
DE EC=2.3.1.75;
DE AltName: Full=Wax synthase 8;
GN OrderedLocusNames=At1g34520; ORFNames=F12K21.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC linear esters (waxes). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
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DR EMBL; AC023279; AAF79269.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31722.1; -; Genomic_DNA.
DR RefSeq; NP_174711.2; NM_103174.3.
DR AlphaFoldDB; Q9LNL1; -.
DR PaxDb; Q9LNL1; -.
DR PRIDE; Q9LNL1; -.
DR EnsemblPlants; AT1G34520.1; AT1G34520.1; AT1G34520.
DR GeneID; 840354; -.
DR Gramene; AT1G34520.1; AT1G34520.1; AT1G34520.
DR KEGG; ath:AT1G34520; -.
DR Araport; AT1G34520; -.
DR TAIR; locus:2009303; AT1G34520.
DR eggNOG; ENOG502S7Z7; Eukaryota.
DR HOGENOM; CLU_045902_0_0_1; -.
DR InParanoid; Q9LNL1; -.
DR OMA; YINRETP; -.
DR OrthoDB; 606079at2759; -.
DR PhylomeDB; Q9LNL1; -.
DR BioCyc; ARA:AT1G34520-MON; -.
DR BRENDA; 2.3.1.75; 399.
DR PRO; PR:Q9LNL1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LNL1; baseline and differential.
DR Genevisible; Q9LNL1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR044851; Wax_synthase.
DR InterPro; IPR032805; Wax_synthase_dom.
DR InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR PANTHER; PTHR31595; PTHR31595; 1.
DR Pfam; PF13813; MBOAT_2; 1.
DR PIRSF; PIRSF037006; Wax_synthase; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..336
FT /note="Probable long-chain-alcohol O-fatty-acyltransferase
FT 8"
FT /id="PRO_0000380684"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 336 AA; 38954 MW; A026C2B99F7A66FD CRC64;
MEEELKSFVK VWGSAIISVS YCYYIPSKIK SGVHRLLSVL PVCVLFLVLP LFFVFTIFSS
TTAFCLSILA NFKLILFAFD KGPLLPLPAN LFRFICFTCL PIKIQKNPNS QNHLPKWVFF
CKAAIFGVLL NVHNYKSSLP PILLICLYPL HLYLVLDVLL TIVNALLTII LGCDLEPHFN
EPYLATSLQD FWGRRWNLMV PAIFRPGVYH PMRSVCQPQM RSDWARFMGC WTTFFVSGLI
HELVYFYINR ETPTWEVTWF FVLQGVCTAM EKAVKRKTRW SLSPMLSRLI TVGFLVVTGY
FLFFRQIERS NMLERRATEA SLIIDFVKHK LSNFLL
