WAXSA_ARATH
ID WAXSA_ARATH Reviewed; 337 AA.
AC Q3ED15; Q9LNK9;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Putative long-chain-alcohol O-fatty-acyltransferase 10;
DE EC=2.3.1.75;
DE AltName: Full=Wax synthase 10;
GN OrderedLocusNames=At1g34490; ORFNames=F12K21.19, F12K21.28;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Catalyzes the final step in the synthesis of long-chain
CC linear esters (waxes). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the wax synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79268.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g34490 and At1g34500.; Evidence={ECO:0000305};
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DR EMBL; AC023279; AAF79268.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31719.1; -; Genomic_DNA.
DR RefSeq; NP_174708.1; NM_103171.2.
DR AlphaFoldDB; Q3ED15; -.
DR PaxDb; Q3ED15; -.
DR PRIDE; Q3ED15; -.
DR EnsemblPlants; AT1G34490.1; AT1G34490.1; AT1G34490.
DR GeneID; 840351; -.
DR Gramene; AT1G34490.1; AT1G34490.1; AT1G34490.
DR KEGG; ath:AT1G34490; -.
DR Araport; AT1G34490; -.
DR TAIR; locus:2009338; AT1G34490.
DR eggNOG; ENOG502S7Z7; Eukaryota.
DR HOGENOM; CLU_045902_0_0_1; -.
DR OrthoDB; 606079at2759; -.
DR PhylomeDB; Q3ED15; -.
DR BioCyc; ARA:AT1G34490-MON; -.
DR BRENDA; 2.3.1.75; 399.
DR PRO; PR:Q3ED15; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q3ED15; baseline and differential.
DR Genevisible; Q3ED15; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR044851; Wax_synthase.
DR InterPro; IPR032805; Wax_synthase_dom.
DR InterPro; IPR017088; Wax_synthase_Magnoliopsida.
DR PANTHER; PTHR31595; PTHR31595; 1.
DR Pfam; PF13813; MBOAT_2; 1.
DR PIRSF; PIRSF037006; Wax_synthase; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..337
FT /note="Putative long-chain-alcohol O-fatty-acyltransferase
FT 10"
FT /id="PRO_0000380686"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 337 AA; 39009 MW; 10CE0E0E0BD710A8 CRC64;
MEEELKSFVK VWGSAIISVS YCYYIPSKIK RGVHRLLSVL PVCVLFLVLP LFFVFTIFSS
TTAFCLSILA NFKLILFAFD KGPLLPLPTN LFRFICFTCL PIKLQTKPNS QNHLPKWVLP
SKVAIFVLLL NIRSYKILLP PILLLGLYPL HLYIVLDVLL TIVNALLTII LRCDLEPHFN
EPYLATSLQD FWGHRWNLMV SAIYRPGVYS PVRSVCQHQM RSDWARFMGC MTTFFVSGLI
HELVYFYINR EKPTLEVTWF FVLHGVCTAM EIAVKRKMQW SLSPMLLRLI TVGFLVVTGD
LLFFGQIERS NMLERRANEA SLFIDFVKRK VFNYTVS
