WBBI_ECOLI
ID WBBI_ECOLI Reviewed; 330 AA.
AC P37749;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Beta-1,6-galactofuranosyltransferase WbbI;
DE EC=2.4.1.-;
DE AltName: Full=D-Galf:alpha-D-Glc beta-1,6-galactofuranosyltransferase;
DE AltName: Full=GalF transferase;
GN Name=wbbI; Synonyms=yefG; OrderedLocusNames=b2034, JW2019;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7517390; DOI=10.1128/jb.176.13.4133-4143.1994;
RA Yao Z., Valvano M.A.;
RT "Genetic analysis of the O-specific lipopolysaccharide biosynthesis region
RT (rfb) of Escherichia coli K-12 W3110: identification of genes that confer
RT group 6 specificity to Shigella flexneri serotypes Y and 4a.";
RL J. Bacteriol. 176:4133-4143(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / WG1;
RX PubMed=7517391; DOI=10.1128/jb.176.13.4144-4156.1994;
RA Stevenson G., Neal B., Liu D., Hobbs M., Packer N.H., Batley M.,
RA Redmond J.W., Lindquist L., Reeves P.R.;
RT "Structure of the O antigen of Escherichia coli K-12 and the sequence of
RT its rfb gene cluster.";
RL J. Bacteriol. 176:4144-4156(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP FUNCTION AS A GALACTOFURANOSYLTRANSFERASE, AND SUBSTRATE SPECIFICITY.
RX PubMed=17047874; DOI=10.1039/b609455d;
RA Wing C., Errey J.C., Mukhopadhyay B., Blanchard J.S., Field R.A.;
RT "Expression and initial characterization of WbbI, a putative D-Galf:alpha-
RT D-Glc beta-1,6-galactofuranosyltransferase from Escherichia coli K-12.";
RL Org. Biomol. Chem. 4:3945-3950(2006).
CC -!- FUNCTION: Involved in the transfer of galactofuranose (Galf) onto an
CC alpha-D-gluco-configured acceptor substrate to form a beta-1,6-linkage.
CC It uses n-octyl alpha-D-glucopyranoside as an acceptor substrate for
CC the addition of galactofuranose from the donor substrate UDP-
CC galactofuranose. It is not able to use beta-D-glucopyranoside isomers.
CC {ECO:0000269|PubMed:17047874}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
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DR EMBL; U03041; AAC31634.1; -; Genomic_DNA.
DR EMBL; U09876; AAB88405.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75095.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15876.1; -; Genomic_DNA.
DR PIR; I69645; I69645.
DR RefSeq; NP_416538.1; NC_000913.3.
DR RefSeq; WP_001407542.1; NZ_LN832404.1.
DR AlphaFoldDB; P37749; -.
DR SMR; P37749; -.
DR BioGRID; 4261355; 264.
DR IntAct; P37749; 2.
DR STRING; 511145.b2034; -.
DR jPOST; P37749; -.
DR PaxDb; P37749; -.
DR PRIDE; P37749; -.
DR EnsemblBacteria; AAC75095; AAC75095; b2034.
DR EnsemblBacteria; BAA15876; BAA15876; BAA15876.
DR GeneID; 947041; -.
DR KEGG; ecj:JW2019; -.
DR KEGG; eco:b2034; -.
DR PATRIC; fig|1411691.4.peg.217; -.
DR EchoBASE; EB1926; -.
DR eggNOG; COG0438; Bacteria.
DR HOGENOM; CLU_057651_1_0_6; -.
DR OMA; FQKSPWL; -.
DR PhylomeDB; P37749; -.
DR BioCyc; EcoCyc:EG11983-MON; -.
DR BioCyc; MetaCyc:EG11983-MON; -.
DR UniPathway; UPA00030; -.
DR PRO; PR:P37749; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008921; F:lipopolysaccharide-1,6-galactosyltransferase activity; IDA:EcoCyc.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
PE 1: Evidence at protein level;
KW Cytoplasm; Lipopolysaccharide biosynthesis; Reference proteome;
KW Transferase.
FT CHAIN 1..330
FT /note="Beta-1,6-galactofuranosyltransferase WbbI"
FT /id="PRO_0000169120"
SQ SEQUENCE 330 AA; 37757 MW; 036A9C3523424BEF CRC64;
MYFLNDLNFS RRDAGFKARK DALDIASDYE NISVVNIPLW GGVVQRIISS VKLSTFLCGL
ENKDVLIFNF PMAKPFWHIL SFFHRLLKFR IVPLIHDIDE LRGGGGSDSV RLATCDMVIS
HNPQMTKYLS KYMSQDKIKD IKIFDYLVSS DVEHRDVTDK QRGVIYAGNL SRHKCSFIYT
EGCDFTLFGV NYENKDNPKY LGSFDAQSPE KINLPGMQFG LIWDGDSVET CSGAFGDYLK
FNNPHKTSLY LSMELPVFIW DKAALADFIV DNRIGYAVGS IKEMQEIVDS MTIETYKQIS
ENTKIISQKI RTGSYFRDVL EEVIDDLKTR
