ID   WBIN_ECOLX              Reviewed;         338 AA.
AC   P0DMP7; Q5J7D6;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   29-OCT-2014, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Probable O-antigen biosynthesis glycosyltransferase WbiN {ECO:0000250|UniProtKB:P0DMP6};
DE            EC=2.4.1.306 {ECO:0000250|UniProtKB:P0DMP6};
DE   AltName: Full=UDP-GalNAc:alpha-D-GalNAc-diphosphoundecaprenol alpha-1,3-N-acetylgalactosaminyltransferase {ECO:0000250|UniProtKB:P0DMP6};
GN   Name=wbiN {ECO:0000312|EMBL:AAR90884.1};
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O127:K63 /B8;
RA   Yi W., Shao J., Wang P.G.;
RT   "Sequence and characterization of Escherichia coli O127 antigen
RT   biosynthesis gene cluster.";
RL   Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the assembly of the O-repeating unit during O-
CC       antigen biosynthesis. {ECO:0000250|UniProtKB:P0DMP6}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-acetyl-alpha-D-galactosaminyl-di-trans,octa-cis-undecaprenyl
CC         diphosphate + UDP-N-acetyl-alpha-D-galactosamine = alpha-D-GalNAc-
CC         (1->3)-alpha-D-GalNAc-di-trans,octa-cis-undecaprenyl diphosphate +
CC         H(+) + UDP; Xref=Rhea:RHEA:36759, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:67138, ChEBI:CHEBI:73987,
CC         ChEBI:CHEBI:74214; EC=2.4.1.306;
CC         Evidence={ECO:0000250|UniProtKB:P0DMP6};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen
CC       biosynthesis. {ECO:0000250|UniProtKB:P0DMP6}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
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DR   EMBL; AY493508; AAR90884.1; -; Genomic_DNA.
DR   RefSeq; WP_000799972.1; NZ_WSXE01000068.1.
DR   AlphaFoldDB; P0DMP7; -.
DR   SMR; P0DMP7; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   UniPathway; UPA00281; -.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009243; P:O antigen biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR028098; Glyco_trans_4-like_N.
DR   Pfam; PF13439; Glyco_transf_4; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipopolysaccharide biosynthesis; Transferase.
FT   CHAIN           1..338
FT                   /note="Probable O-antigen biosynthesis glycosyltransferase
FT                   WbiN"
FT                   /id="PRO_0000430648"
SQ   SEQUENCE   338 AA;  38148 MW;  C3A813A3EE248251 CRC64;
     MKNVGFIVTK SEIGGAQTWV NEISNLIKEE CNIFLITSEE GWLTHKDVFA GVFVIPGIKK
     YFDFLTLFKL RKILKENNIS TLIASSANAG VYARLVRLLV DFKCIYVSHG WSCLYNGGRL
     KSIFCIVEKY LSLLTDVIWC VSKSDEKKAI ENIGIKEPKI ITVSNSVPQM PRCNNKQLQY
     KVLFVGRLTH PKRPELLANV ISKKPQYSLH IVGGGERLES LKKQFSECEN IHFLGEVNNF
     YNYHEYDLFS LISDSEGLPM SGLEAHTAAI PLLLSDVGGC FELIEGNGLL VENTEDDIGY
     KLDKIFDDYE NYREQAIRAS GKFVIENYAS AYKSIILG