WBPI_PSEAE
ID WBPI_PSEAE Reviewed; 354 AA.
AC G3XD61; P72142;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 03-AUG-2022, entry version 57.
DE RecName: Full=UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronate 2-epimerase;
DE Short=UDP-alpha-D-GlcNAc3NAcA 2-epimerase {ECO:0000303|PubMed:17346239};
DE EC=5.1.3.23 {ECO:0000269|PubMed:17346239};
DE AltName: Full=UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerase {ECO:0000303|PubMed:17346239};
GN Name=wbpI {ECO:0000312|EMBL:AAG06536.1}; OrderedLocusNames=PA3148;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAC45863.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PATHWAY.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1 {ECO:0000312|EMBL:AAC45863.1};
RX PubMed=8939432; DOI=10.1046/j.1365-2958.1996.1351503.x;
RA Burrows L.L., Charter D.F., Lam J.S.;
RT "Molecular characterization of the Pseudomonas aeruginosa serotype O5
RT (PAO1) B-band lipopolysaccharide gene cluster.";
RL Mol. Microbiol. 22:481-495(1996).
RN [2] {ECO:0000312|EMBL:AAG06536.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3] {ECO:0000305}
RP FUNCTION, AND PATHWAY.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1 {ECO:0000269|PubMed:10930727};
RX PubMed=10930727; DOI=10.1111/j.1574-6968.2000.tb09219.x;
RA Burrows L.L., Pigeon K.E., Lam J.S.;
RT "Pseudomonas aeruginosa B-band lipopolysaccharide genes wbpA and wbpI and
RT their Escherichia coli homologues wecC and wecB are not functionally
RT interchangeable.";
RL FEMS Microbiol. Lett. 189:135-141(2000).
RN [4] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL
RP PROPERTIES, AND PATHWAY.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1 {ECO:0000269|PubMed:17346239};
RX PubMed=17346239; DOI=10.1042/bj20070017;
RA Westman E.L., McNally D.J., Rejzek M., Miller W.L., Kannathasan V.S.,
RA Preston A., Maskell D.J., Field R.A., Brisson J.R., Lam J.S.;
RT "Identification and biochemical characterization of two novel UDP-2,3-
RT diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerases from
RT respiratory pathogens.";
RL Biochem. J. 405:123-130(2007).
CC -!- FUNCTION: Plays a role in the biosynthesis of B-band O antigen for
CC serotype O5. Catalyzes the epimerization of UDP-2,3-diacetamido-2,3-
CC dideoxy-alpha-D-glucuronic acid (UDP-alpha-D-GlcNAc3NAcA) to UDP-2,3-
CC diacetamido-2,3-dideoxy-alpha-D-mannuronic acid (UDP-alpha-D-
CC ManNAc3NAcA). Exhibits high specificity towards the substrate as UDP-
CC alpha-D-GlcNAc, UDP-alpha-D-GlcNAcA (UDP-2-acetamido-2-deoxy-alpha-D-
CC glucuronic acid) and UDP-alpha-D-GlcNAc3NAc (UDP-2,3-diacetamido-2,3-
CC dideoxy-alpha-D-glucose) cannot act as substrates.
CC {ECO:0000269|PubMed:10930727, ECO:0000269|PubMed:17346239}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate = UDP-2,3-
CC diacetamido-2,3-dideoxy-alpha-D-mannuronate; Xref=Rhea:RHEA:24764,
CC ChEBI:CHEBI:58745, ChEBI:CHEBI:58746; EC=5.1.3.23;
CC Evidence={ECO:0000269|PubMed:17346239};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 6.0. {ECO:0000269|PubMed:17346239};
CC Temperature dependence:
CC Optimum temperature is 30-37 degrees Celsius.
CC {ECO:0000269|PubMed:17346239};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen
CC biosynthesis. {ECO:0000269|PubMed:10930727,
CC ECO:0000269|PubMed:17346239, ECO:0000269|PubMed:8939432}.
CC -!- SIMILARITY: Belongs to the UDP-N-acetylglucosamine 2-epimerase family.
CC {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC45863.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U50396; AAC45863.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE004091; AAG06536.1; -; Genomic_DNA.
DR PIR; C83253; C83253.
DR RefSeq; NP_251838.1; NC_002516.2.
DR RefSeq; WP_003113418.1; NZ_QZGE01000023.1.
DR AlphaFoldDB; G3XD61; -.
DR SMR; G3XD61; -.
DR STRING; 208964.PA3148; -.
DR PaxDb; G3XD61; -.
DR DNASU; 882693; -.
DR EnsemblBacteria; AAG06536; AAG06536; PA3148.
DR GeneID; 882693; -.
DR KEGG; pae:PA3148; -.
DR PATRIC; fig|208964.12.peg.3297; -.
DR PseudoCAP; PA3148; -.
DR HOGENOM; CLU_041674_0_1_6; -.
DR InParanoid; G3XD61; -.
DR OMA; RYNTERP; -.
DR PhylomeDB; G3XD61; -.
DR BioCyc; MetaCyc:MON-17576; -.
DR BioCyc; PAER208964:G1FZ6-3208-MON; -.
DR UniPathway; UPA00281; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0050378; F:UDP-glucuronate 4-epimerase activity; EXP:PseudoCAP.
DR GO; GO:0008761; F:UDP-N-acetylglucosamine 2-epimerase activity; IDA:UniProtKB.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009243; P:O antigen biosynthetic process; IMP:PseudoCAP.
DR GO; GO:0000271; P:polysaccharide biosynthetic process; IMP:PseudoCAP.
DR InterPro; IPR003331; UDP_GlcNAc_Epimerase_2_dom.
DR InterPro; IPR029767; WecB-like.
DR PANTHER; PTHR43174; PTHR43174; 1.
DR Pfam; PF02350; Epimerase_2; 1.
DR TIGRFAMs; TIGR00236; wecB; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Isomerase;
KW Lipopolysaccharide biosynthesis; Reference proteome.
FT CHAIN 1..354
FT /note="UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronate 2-
FT epimerase"
FT /id="PRO_0000419618"
SQ SEQUENCE 354 AA; 38907 MW; ADFDBFFA3348784B CRC64;
MKILTIIGAR PQFIKASVVS KAIIEQQTLS EIIVHTGQHF DANMSEIFFE QLGIPKPDYQ
LDIHGGTHGQ MTGRMLMEIE DVILKEKPHR VLVYGDTNST LAGALAASKL HVPIAHIEAG
LRSFNMRMPE EINRILTDQV SDILFCPTRV AIDNLKNEGF ERKAAKIVNV GDVMQDSALF
FAQRATSPIG LASQDGFILA TLHRAENTDD PVRLTSIVEA LNEIQINVAP VVLPLHPRTR
GVIERLGLKL EVQVIDPVGY LEMIWLLQRS GLVLTDSGGV QKEAFFFGKP CVTMRDQTEW
VELVTCGANV LVGAARDMIV ESARTSLGKT IQDDGQLYGG GQASSRIAEY LAKL
