ID   WC2_NEUCR               Reviewed;         530 AA.
AC   P78714; Q7RVJ5;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=White collar 2 protein;
DE            Short=WC2;
GN   Name=wc-2; ORFNames=B13C5.120, NCU00902;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DNA-BINDING, AND MUTAGENESIS
RP   OF GLY-14 AND GLY-485.
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=9009271; DOI=10.1093/emboj/16.1.98;
RA   Linden H., Macino G.;
RT   "White collar 2, a partner in blue-light signal transduction, controlling
RT   expression of light-regulated genes in Neurospora crassa.";
RL   EMBO J. 16:98-109(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: May function as a transcription factor involved in light
CC       regulation. Binds and affects blue light regulation of the al-3 gene.
CC       Wc-1 and wc-2 interact via homologous PAS domains, bind to promoters of
CC       light regulated genes such as frq, and activate transcription. May bind
CC       directly to frq. {ECO:0000269|PubMed:9009271}.
CC   -!- SUBUNIT: Heterodimer of wc-1 and wc-2 (Potential). Binds to DNA.
CC       {ECO:0000305}.
CC   -!- INTERACTION:
CC       P78714; Q01371: wc-1; NbExp=5; IntAct=EBI-2924174, EBI-2922603;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- INDUCTION: By blue light.
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DR   EMBL; Y09119; CAA70336.1; -; Genomic_DNA.
DR   EMBL; BX842682; CAE81996.1; -; Genomic_DNA.
DR   EMBL; CM002236; EAA34583.3; -; Genomic_DNA.
DR   PIR; T46648; T46648.
DR   RefSeq; XP_963819.3; XM_958726.3.
DR   AlphaFoldDB; P78714; -.
DR   SMR; P78714; -.
DR   DIP; DIP-1156N; -.
DR   IntAct; P78714; 3.
DR   MINT; P78714; -.
DR   STRING; 5141.EFNCRP00000000830; -.
DR   EnsemblFungi; EAA34583; EAA34583; NCU00902.
DR   GeneID; 3879968; -.
DR   KEGG; ncr:NCU00902; -.
DR   VEuPathDB; FungiDB:NCU00902; -.
DR   HOGENOM; CLU_024414_0_0_1; -.
DR   InParanoid; P78714; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00130; PAS; 1.
DR   CDD; cd00202; ZnF_GATA; 1.
DR   Gene3D; 3.30.50.10; -; 1.
DR   InterPro; IPR000014; PAS.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   InterPro; IPR013655; PAS_fold_3.
DR   InterPro; IPR000679; Znf_GATA.
DR   InterPro; IPR013088; Znf_NHR/GATA.
DR   Pfam; PF00320; GATA; 1.
DR   Pfam; PF08447; PAS_3; 1.
DR   SMART; SM00091; PAS; 1.
DR   SMART; SM00401; ZnF_GATA; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
DR   TIGRFAMs; TIGR00229; sensory_box; 1.
DR   PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR   PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
DR   PROSITE; PS50112; PAS; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..530
FT                   /note="White collar 2 protein"
FT                   /id="PRO_0000083490"
FT   REPEAT          9..12
FT                   /note="1"
FT   REPEAT          21..24
FT                   /note="2"
FT   REPEAT          25..28
FT                   /note="3"
FT   REPEAT          29..32
FT                   /note="4"
FT   REPEAT          33..36
FT                   /note="5"
FT   REPEAT          37..40
FT                   /note="6"
FT   REPEAT          41..44
FT                   /note="7"
FT   DOMAIN          162..232
FT                   /note="PAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT   ZN_FING         468..493
FT                   /note="GATA-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT   REGION          9..44
FT                   /note="7 X 4 AA repeats of G-[SAT]-G-M"
FT   REGION          134..158
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          315..343
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          504..530
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        315..332
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         14
FT                   /note="G->S: In 234W."
FT                   /evidence="ECO:0000269|PubMed:9009271"
FT   MUTAGEN         485
FT                   /note="G->E: In ER33."
FT                   /evidence="ECO:0000269|PubMed:9009271"
SQ   SEQUENCE   530 AA;  56895 MW;  B667BD3171380A18 CRC64;
     MSHGQPPPGS SMYGFGAMGM GSGMGSGMGS GMGTGMGTGM GTGMSASQMT SDPQDMMSLL
     DTSVFPGFDG MSMSLDVGDS MSNPFTPVSV PPPLPAGNAG PSHVGVCGGH GAPDQLFSPD
     DLIATSMSSA GPMIATPTTT TSGPSGGPSS GGGSTLTEFT KRRNWPAKVV EELQDWEHIL
     DANGRIKHVS PSVEPLTGYK PPEIIDLFLR DLIHPDDVGV FTAELNEAIA TGSQLRLFYR
     FRKKDGNWTI FETVGHAHIA AAKFAPNPQN QSPFCQAVFM MARPYPTKNA GLLDSFLEHK
     IENERLKRRI AELRREEQEE QEESHRTWRM SQEGRSDVTP SDDTATQMGM TPFYIPMNAQ
     ADVMMPPPSQ PASSLNIALT RENLEGIAGS RPDSIREKML RYEGNHADTI EMLTGLKYQE
     GERSHGITTG NASPTLIKGD AGIAIPLDRD PRTGEKKKKI KVAEEYVCTD CGTLDSPEWR
     KGPSGPKTLC NACGLRWAKK EKKKNANNNN NGGGIGGHND IHTPMGDHMG