ID   WCAA_ECOLI              Reviewed;         279 AA.
AC   P77414;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Putative colanic acid biosynthesis glycosyl transferase WcaA;
GN   Name=wcaA; OrderedLocusNames=b2059, JW2044;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8759852; DOI=10.1128/jb.178.16.4885-4893.1996;
RA   Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.;
RT   "Organization of the Escherichia coli K-12 gene cluster responsible for
RT   production of the extracellular polysaccharide colanic acid.";
RL   J. Bacteriol. 178:4885-4893(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- PATHWAY: Slime biogenesis; slime polysaccharide biosynthesis.
CC   -!- SIMILARITY: To R.meliloti ExoO. {ECO:0000305}.
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DR   EMBL; U38473; AAC77836.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75120.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15912.1; -; Genomic_DNA.
DR   PIR; B64972; B64972.
DR   RefSeq; NP_416563.1; NC_000913.3.
DR   RefSeq; WP_000794699.1; NZ_LN832404.1.
DR   AlphaFoldDB; P77414; -.
DR   SMR; P77414; -.
DR   BioGRID; 4259687; 320.
DR   IntAct; P77414; 8.
DR   STRING; 511145.b2059; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   PaxDb; P77414; -.
DR   PRIDE; P77414; -.
DR   EnsemblBacteria; AAC75120; AAC75120; b2059.
DR   EnsemblBacteria; BAA15912; BAA15912; BAA15912.
DR   GeneID; 946570; -.
DR   KEGG; ecj:JW2044; -.
DR   KEGG; eco:b2059; -.
DR   PATRIC; fig|1411691.4.peg.192; -.
DR   EchoBASE; EB3339; -.
DR   eggNOG; COG0463; Bacteria.
DR   HOGENOM; CLU_025996_0_5_6; -.
DR   InParanoid; P77414; -.
DR   OMA; CVMVNEG; -.
DR   PhylomeDB; P77414; -.
DR   BioCyc; EcoCyc:G7104-MON; -.
DR   BioCyc; MetaCyc:G7104-MON; -.
DR   UniPathway; UPA00936; -.
DR   PRO; PR:P77414; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0015020; F:glucuronosyltransferase activity; IDA:EcoCyc.
DR   GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0009242; P:colanic acid biosynthetic process; IGC:EcoCyc.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0045228; P:slime layer polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR024009; Colanic_acid_synth_WcaA.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
DR   TIGRFAMs; TIGR04017; WcaA; 1.
PE   4: Predicted;
KW   Lipopolysaccharide biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..279
FT                   /note="Putative colanic acid biosynthesis glycosyl
FT                   transferase WcaA"
FT                   /id="PRO_0000065953"
SQ   SEQUENCE   279 AA;  33035 MW;  75F74D30026E327A CRC64;
     MKNNPLISIY MPTWNRQQLA IRAIKSVLRQ DYSNWEMIIV DDCSTSWEQL QQYVTALNDP
     RITYIHNDIN SGACAVRNQA IMLAQGEYIT GIDDDDEWTP NRLSVFLAHK QQLVTHAFLY
     ANDYVCQGEV YSQPASLPLY PKSPYSRRLF YKRNIIGNQV FTWAWRFKEC LFDTELKAAQ
     DYDIFLRMVV EYGEPWKVEE ATQILHINHG EMQITSSPKK FSGYFHFYRK HKDKFDRASK
     KYQLFTLYQI RNKRMTWRTL LTLLSVRNGK RLADGIRGR