WCAD_ECOLI
ID WCAD_ECOLI Reviewed; 405 AA.
AC P71238; P76385; Q2MAY3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Putative colanic acid polymerase;
GN Name=wcaD; OrderedLocusNames=b2056, JW2041;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8759852; DOI=10.1128/jb.178.16.4885-4893.1996;
RA Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.;
RT "Organization of the Escherichia coli K-12 gene cluster responsible for
RT production of the extracellular polysaccharide colanic acid.";
RL J. Bacteriol. 178:4885-4893(1996).
RN [2]
RP SEQUENCE REVISION.
RC STRAIN=K12;
RA Reeves P.R.;
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- PATHWAY: Slime biogenesis; slime polysaccharide biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
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DR EMBL; U38473; AAC77839.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75117.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76573.1; -; Genomic_DNA.
DR PIR; G64971; G64971.
DR RefSeq; NP_416560.1; NC_000913.3.
DR RefSeq; WP_000107816.1; NZ_STEB01000002.1.
DR AlphaFoldDB; P71238; -.
DR BioGRID; 4259688; 157.
DR DIP; DIP-11120N; -.
DR IntAct; P71238; 1.
DR STRING; 511145.b2056; -.
DR PaxDb; P71238; -.
DR PRIDE; P71238; -.
DR EnsemblBacteria; AAC75117; AAC75117; b2056.
DR EnsemblBacteria; BAE76573; BAE76573; BAE76573.
DR GeneID; 66674046; -.
DR GeneID; 946550; -.
DR KEGG; ecj:JW2041; -.
DR KEGG; eco:b2056; -.
DR PATRIC; fig|511145.12.peg.2133; -.
DR EchoBASE; EB3342; -.
DR eggNOG; ENOG502ZA5M; Bacteria.
DR HOGENOM; CLU_056700_0_0_6; -.
DR OMA; WRSMSTS; -.
DR PhylomeDB; P71238; -.
DR BioCyc; EcoCyc:G7101-MON; -.
DR BioCyc; MetaCyc:G7101-MON; -.
DR UniPathway; UPA00936; -.
DR PRO; PR:P71238; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0046377; P:colanic acid metabolic process; IGC:EcoCyc.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0045228; P:slime layer polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR024013; Colanic_acid_synth_WcaD.
DR TIGRFAMs; TIGR04010; WcaD; 1.
PE 4: Predicted;
KW Cell inner membrane; Cell membrane; Lipopolysaccharide biosynthesis;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..405
FT /note="Putative colanic acid polymerase"
FT /id="PRO_0000065955"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 108
FT /note="P -> A (in Ref. 2; AAC77839)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 405 AA; 45409 MW; A3D9D91255686043 CRC64;
MSTSIRICSY LLLPLIYLLV NVKIAQLGES FPITIVTFLP VLLLLFLERI SVKKLMIALG
IGAGLTAFNY LFGQSLDASK YVTSTMLFVY IVIIIGMVWS IRFKTISPHN HRKILRFFYL
VVGLVVALAA VEMAQIILTG GSSIMESISK YLIYSNSYVL NFIKFGGKRT TALYFEPAFF
ALALISIWLS IKQFGIKTPK TDAMILAGII LSGSFSGVMT FILFYLLEWA FQYLNKEAIK
KKLPLALISL AVFLVGVVIA FPYISTRLGD LGTEGSSSYY RIVGPLVMVG YSLTHIDGVV
RFGSLYEYVA SFGIFNGADV GKTIDNGLYL LIIYFSWFAV FLSLWYMGKV IKMMINAFGD
NRNFRVQLYL FTPVSLFFTG SIFSPEYAFL IVCPFILRKA LNITR
