ID   WCAF_ECOLI              Reviewed;         182 AA.
AC   P0ACD2; P71240; P76383;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Putative colanic acid biosynthesis acetyltransferase WcaF;
DE            EC=2.3.1.-;
GN   Name=wcaF; OrderedLocusNames=b2054, JW2039;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8759852; DOI=10.1128/jb.178.16.4885-4893.1996;
RA   Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.;
RT   "Organization of the Escherichia coli K-12 gene cluster responsible for
RT   production of the extracellular polysaccharide colanic acid.";
RL   J. Bacteriol. 178:4885-4893(1996).
RN   [2]
RP   SEQUENCE REVISION TO N-TERMINUS.
RC   STRAIN=K12;
RA   Reeves P.R.;
RL   Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- PATHWAY: Slime biogenesis; slime polysaccharide biosynthesis.
CC   -!- SIMILARITY: Belongs to the transferase hexapeptide repeat family.
CC       {ECO:0000305}.
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DR   EMBL; U38473; AAC77841.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75115.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15910.1; -; Genomic_DNA.
DR   PIR; E64971; E64971.
DR   RefSeq; NP_416558.1; NC_000913.3.
DR   RefSeq; WP_001153547.1; NZ_STEB01000002.1.
DR   AlphaFoldDB; P0ACD2; -.
DR   SMR; P0ACD2; -.
DR   BioGRID; 4259689; 200.
DR   IntAct; P0ACD2; 1.
DR   STRING; 511145.b2054; -.
DR   PaxDb; P0ACD2; -.
DR   PRIDE; P0ACD2; -.
DR   EnsemblBacteria; AAC75115; AAC75115; b2054.
DR   EnsemblBacteria; BAA15910; BAA15910; BAA15910.
DR   GeneID; 58459758; -.
DR   GeneID; 946578; -.
DR   KEGG; ecj:JW2039; -.
DR   KEGG; eco:b2054; -.
DR   PATRIC; fig|1411691.4.peg.197; -.
DR   EchoBASE; EB3344; -.
DR   eggNOG; COG0110; Bacteria.
DR   HOGENOM; CLU_051638_7_2_6; -.
DR   InParanoid; P0ACD2; -.
DR   OMA; IWAPWNL; -.
DR   PhylomeDB; P0ACD2; -.
DR   BioCyc; EcoCyc:G7099-MON; -.
DR   BioCyc; MetaCyc:G7099-MON; -.
DR   UniPathway; UPA00936; -.
DR   PRO; PR:P0ACD2; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016413; F:O-acetyltransferase activity; IDA:EcoCyc.
DR   GO; GO:0008374; F:O-acyltransferase activity; IBA:GO_Central.
DR   GO; GO:0009242; P:colanic acid biosynthetic process; IMP:EcoCyc.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0044010; P:single-species biofilm formation; IMP:EcoCyc.
DR   GO; GO:0045228; P:slime layer polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR024005; Colanic_acid_synth_WcaF.
DR   InterPro; IPR001451; Hexapep.
DR   InterPro; IPR018357; Hexapep_transf_CS.
DR   InterPro; IPR011004; Trimer_LpxA-like_sf.
DR   Pfam; PF00132; Hexapep; 1.
DR   SUPFAM; SSF51161; SSF51161; 1.
DR   TIGRFAMs; TIGR04008; WcaF; 1.
DR   PROSITE; PS00101; HEXAPEP_TRANSFERASES; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Lipopolysaccharide biosynthesis; Reference proteome;
KW   Repeat; Transferase.
FT   CHAIN           1..182
FT                   /note="Putative colanic acid biosynthesis acetyltransferase
FT                   WcaF"
FT                   /id="PRO_0000068736"
FT   CONFLICT        50
FT                   /note="F -> L (in Ref. 2; AAC77841)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   182 AA;  19962 MW;  8AA5E672CA802442 CRC64;
     MQDLSGFSVP KGFRGGNAIK VQLWWAVQAT IFAWSPQVLY RWRAFLLRLF GAKIGKNVVI
     RPSVKITYPW KLTLGDYAWV GDDVNLYTLG EITIGAHSVI SQKSYLCTGS HDHASQHFTI
     NATPIVIGEK CWLATDVFVA PGVTIGDGTV VGARSSVFKS LPANVVCRGN PAVVIRERVE
     TE