WCAI_ECOLI
ID WCAI_ECOLI Reviewed; 407 AA.
AC P32057;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Putative colanic acid biosynthesis glycosyl transferase WcaI;
GN Name=wcaI; Synonyms=yefD; OrderedLocusNames=b2050, JW2035;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7815923; DOI=10.1093/oxfordjournals.molbev.a040166;
RA Aoyama K., Haase A.M., Reeves P.R.;
RT "Evidence for effect of random genetic drift on G+C content after lateral
RT transfer of fucose pathway genes to Escherichia coli K-12.";
RL Mol. Biol. Evol. 11:829-838(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8759852; DOI=10.1128/jb.178.16.4885-4893.1996;
RA Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.;
RT "Organization of the Escherichia coli K-12 gene cluster responsible for
RT production of the extracellular polysaccharide colanic acid.";
RL J. Bacteriol. 178:4885-4893(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- PATHWAY: Slime biogenesis; slime polysaccharide biosynthesis.
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DR EMBL; U38473; AAC77845.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75111.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15906.1; -; Genomic_DNA.
DR PIR; F55239; F55239.
DR RefSeq; NP_416554.1; NC_000913.3.
DR RefSeq; WP_000699693.1; NZ_LN832404.1.
DR AlphaFoldDB; P32057; -.
DR SMR; P32057; -.
DR BioGRID; 4263310; 342.
DR DIP; DIP-11124N; -.
DR IntAct; P32057; 3.
DR STRING; 511145.b2050; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR PaxDb; P32057; -.
DR PRIDE; P32057; -.
DR DNASU; 946588; -.
DR EnsemblBacteria; AAC75111; AAC75111; b2050.
DR EnsemblBacteria; BAA15906; BAA15906; BAA15906.
DR GeneID; 946588; -.
DR KEGG; ecj:JW2035; -.
DR KEGG; eco:b2050; -.
DR PATRIC; fig|1411691.4.peg.201; -.
DR EchoBASE; EB1738; -.
DR eggNOG; COG0297; Bacteria.
DR HOGENOM; CLU_009583_11_5_6; -.
DR InParanoid; P32057; -.
DR OMA; WMAREGH; -.
DR PhylomeDB; P32057; -.
DR BioCyc; EcoCyc:EG11790-MON; -.
DR BioCyc; MetaCyc:EG11790-MON; -.
DR UniPathway; UPA00936; -.
DR PRO; PR:P32057; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0046920; F:alpha-(1->3)-fucosyltransferase activity; IDA:EcoCyc.
DR GO; GO:0009242; P:colanic acid biosynthetic process; IGC:EcoCyc.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0045228; P:slime layer polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR023910; Colanic_acid_synth_WcaI.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR028098; Glyco_trans_4-like_N.
DR Pfam; PF13579; Glyco_trans_4_4; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR TIGRFAMs; TIGR04007; wcaI; 1.
PE 4: Predicted;
KW Lipopolysaccharide biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..407
FT /note="Putative colanic acid biosynthesis glycosyl
FT transferase WcaI"
FT /id="PRO_0000065957"
SQ SEQUENCE 407 AA; 44914 MW; 6EC5AA8D6DFB4962 CRC64;
MKILVYGINY SPELTGIGKY TGEMVEWLAA QGHEVRVITA PPYYPQWQVG ENYSAWRYKR
EEGAATVWRC PLYVPKQPST LKRLLHLGSF AVSSFFPLMA QRRWKPDRII GVVPTLFCAP
GMRLLAKLSG ARTVLHIQDY EVDAMLGLGL AGKGKGGKVA QLATAFERSG LHNVDNVSTI
SRSMMNKAIE KGVAAENVIF FPNWSEIARF QHVADADVDA LRNQLDLPDN KKIILYSGNI
GEKQGLENVI EAADRLRDEP LIFAIVGQGG GKARLEKMAQ QRGLRNMQFF PLQSYDALPA
LLKMGDCHLV VQKRGAADAV LPSKLTNILA VGGNAVITAE AYTELGQLCE TFPGIAVCVE
PESVEALVAG IRQALLLPKH NTVAREYAER TLDKENVLRQ FINDIRG
