ID   WCAK_ECOLI              Reviewed;         426 AA.
AC   P71242; P76380; Q2MAY5;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Colanic acid biosynthesis protein WcaK;
DE            EC=2.-.-.-;
GN   Name=wcaK; OrderedLocusNames=b2045, JW2030;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8759852; DOI=10.1128/jb.178.16.4885-4893.1996;
RA   Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.;
RT   "Organization of the Escherichia coli K-12 gene cluster responsible for
RT   production of the extracellular polysaccharide colanic acid.";
RL   J. Bacteriol. 178:4885-4893(1996).
RN   [2]
RP   SEQUENCE REVISION.
RC   STRAIN=K12;
RA   Reeves P.R.;
RL   Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- PATHWAY: Slime biogenesis; slime polysaccharide biosynthesis.
CC   -!- SIMILARITY: Belongs to the polysaccharide pyruvyl transferase family.
CC       {ECO:0000305}.
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DR   EMBL; U38473; AAC77850.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75106.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76571.1; -; Genomic_DNA.
DR   PIR; D64970; D64970.
DR   RefSeq; NP_416549.1; NC_000913.3.
DR   RefSeq; WP_000770780.1; NZ_LN832404.1.
DR   AlphaFoldDB; P71242; -.
DR   BioGRID; 4261185; 158.
DR   IntAct; P71242; 21.
DR   STRING; 511145.b2045; -.
DR   PaxDb; P71242; -.
DR   PRIDE; P71242; -.
DR   EnsemblBacteria; AAC75106; AAC75106; b2045.
DR   EnsemblBacteria; BAE76571; BAE76571; BAE76571.
DR   GeneID; 946569; -.
DR   KEGG; ecj:JW2030; -.
DR   KEGG; eco:b2045; -.
DR   PATRIC; fig|1411691.4.peg.206; -.
DR   EchoBASE; EB3347; -.
DR   eggNOG; COG2327; Bacteria.
DR   HOGENOM; CLU_741661_0_0_6; -.
DR   InParanoid; P71242; -.
DR   OMA; IMQQLGM; -.
DR   PhylomeDB; P71242; -.
DR   BioCyc; EcoCyc:G7096-MON; -.
DR   BioCyc; MetaCyc:G7096-MON; -.
DR   UniPathway; UPA00936; -.
DR   PRO; PR:P71242; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0045228; P:slime layer polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR023918; Colanic_acid_synth_WcaK.
DR   InterPro; IPR007345; Polysacch_pyruvyl_Trfase.
DR   Pfam; PF04230; PS_pyruv_trans; 1.
DR   TIGRFAMs; TIGR04006; wcaK; 1.
PE   3: Inferred from homology;
KW   Lipopolysaccharide biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..426
FT                   /note="Colanic acid biosynthesis protein WcaK"
FT                   /id="PRO_0000073216"
FT   CONFLICT        77
FT                   /note="K -> I (in Ref. 2; AAC77850)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   426 AA;  47344 MW;  F0B7D87A9A3E594C CRC64;
     MKLLILGNHT CGNRGDSAIL RGLLDAINIL NPHAEVDVMS RYPVSSSWLL NRPVMGDPLF
     LQMKQHNSAA GVVGRVKKVL RRRYQHQVLL SRVTDTGKLR NIAIAQGFTD FVRLLSGYDA
     IIQVGGSFFV DLYGVPQFEH ALCTFMAKKP LFMIGHSVGP FQDEQFNQLA NYVFGHCDAL
     ILRESVSFDL MKRSNITTAK VEHGVDTAWL VDHHTEDFTA SYAVQHWLDV AAQQKTVAIT
     LRELAPFDKR LGTTQQAYEK AFAGVVNRIL DEGYQVIALS TCTGIDSYNK DDRMVALNLR
     QHISDPARYH VVMDELNDLE MGKILGACEL TVGTRLHSAI ISMNFATPAI AINYEHKSAG
     IMQQLGLPEM AIDIRHLLDG SLQAMVADTL GQLPALNARL SEAVSRERQT GMQMVQSVLE
     RIGEVK