WCAL_ECOLI
ID WCAL_ECOLI Reviewed; 406 AA.
AC P71243; P76379;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Putative colanic acid biosynthesis glycosyltransferase WcaL;
DE EC=2.4.-.-;
GN Name=wcaL; Synonyms=yefL; OrderedLocusNames=b2044, JW2029;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8759852; DOI=10.1128/jb.178.16.4885-4893.1996;
RA Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.;
RT "Organization of the Escherichia coli K-12 gene cluster responsible for
RT production of the extracellular polysaccharide colanic acid.";
RL J. Bacteriol. 178:4885-4893(1996).
RN [2]
RP SEQUENCE REVISION.
RC STRAIN=K12;
RA Reeves P.R.;
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- PATHWAY: Slime biogenesis; slime polysaccharide biosynthesis.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC Glycosyltransferase 4 subfamily. {ECO:0000305}.
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DR EMBL; U38473; AAC77851.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75105.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15898.1; -; Genomic_DNA.
DR PIR; C64970; C64970.
DR RefSeq; NP_416548.1; NC_000913.3.
DR RefSeq; WP_000862592.1; NZ_LN832404.1.
DR AlphaFoldDB; P71243; -.
DR SMR; P71243; -.
DR BioGRID; 4259676; 168.
DR DIP; DIP-11127N; -.
DR IntAct; P71243; 1.
DR STRING; 511145.b2044; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR PaxDb; P71243; -.
DR PRIDE; P71243; -.
DR DNASU; 946565; -.
DR EnsemblBacteria; AAC75105; AAC75105; b2044.
DR EnsemblBacteria; BAA15898; BAA15898; BAA15898.
DR GeneID; 946565; -.
DR KEGG; ecj:JW2029; -.
DR KEGG; eco:b2044; -.
DR PATRIC; fig|1411691.4.peg.207; -.
DR EchoBASE; EB2522; -.
DR eggNOG; COG0438; Bacteria.
DR HOGENOM; CLU_009583_14_3_6; -.
DR InParanoid; P71243; -.
DR OMA; RIALRGW; -.
DR PhylomeDB; P71243; -.
DR BioCyc; EcoCyc:G7095-MON; -.
DR UniPathway; UPA00936; -.
DR PRO; PR:P71243; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0046377; P:colanic acid metabolic process; IMP:EcoCyc.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0045228; P:slime layer polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR023884; Colanic_acid_synth_WcaL.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR028098; Glyco_trans_4-like_N.
DR Pfam; PF13439; Glyco_transf_4; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR TIGRFAMs; TIGR04005; wcaL; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Lipopolysaccharide biosynthesis; Reference proteome;
KW Transferase.
FT CHAIN 1..406
FT /note="Putative colanic acid biosynthesis
FT glycosyltransferase WcaL"
FT /id="PRO_0000080311"
FT CONFLICT 126
FT /note="F -> Y (in Ref. 2; AAC77851)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="A -> T (in Ref. 2; AAC77851)"
FT /evidence="ECO:0000305"
FT CONFLICT 144
FT /note="G -> A (in Ref. 2; AAC77851)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="G -> A (in Ref. 2; AAC77851)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 406 AA; 45362 MW; EBC64DB398677D65 CRC64;
MKVGFFLLKF PLSSETFVLN QITAFIDMGF EVEILALQKG DTQNTHAAWT KYNLAARTRW
LQDEPTGKVA KLRHRASQTL RGIHRKNTWQ ALNLKRYGAE SRNLILSAIC GQVATPFRAD
VFIAHFGPAG VTAAKLRELG VIRGKIATIF HGIDISSREV LNHYTPEYQQ LFRRGDLMLP
ISDLWAGRLQ KMGCPREKIA VSRMGVDMTR FSPRPVKAPA TPLEIISVAR LTEKKGLHVA
IEACRQLKEQ GVAFRYRILG IGPWERRLRT LIEQYQLEDV VEMPGFKPSH EVKAMLDDAD
VFLLPSVTGA DGDMEGIPVA LMEAMAVGIP VVSTLHSGIP ELVEADKSGW LVPENDARAL
AQRLAAFSQL DTDELAPVVK RAREKVEHDF NQQVINRELA SLLQAL
