ID   WCAM_ECOLI              Reviewed;         464 AA.
AC   P71244; P76378;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Colanic acid biosynthesis protein WcaM;
DE   Flags: Precursor;
GN   Name=wcaM; Synonyms=yefK; OrderedLocusNames=b2043, JW2028;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8759852; DOI=10.1128/jb.178.16.4885-4893.1996;
RA   Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R.;
RT   "Organization of the Escherichia coli K-12 gene cluster responsible for
RT   production of the extracellular polysaccharide colanic acid.";
RL   J. Bacteriol. 178:4885-4893(1996).
RN   [2]
RP   SEQUENCE REVISION.
RC   STRAIN=K12;
RA   Reeves P.R.;
RL   Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   EXPORT VIA THE TAT-SYSTEM.
RX   PubMed=17218314; DOI=10.1074/jbc.m610507200;
RA   Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B.,
RA   Palmer T., Georgiou G.;
RT   "Export pathway selectivity of Escherichia coli twin arginine translocation
RT   signal peptides.";
RL   J. Biol. Chem. 282:8309-8316(2007).
CC   -!- PATHWAY: Slime biogenesis; slime polysaccharide biosynthesis.
CC   -!- PTM: Exported by the Tat system. The position of the signal peptide
CC       cleavage has not been experimentally proven.
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DR   EMBL; U38473; AAC77852.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75104.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15897.1; -; Genomic_DNA.
DR   PIR; B64970; B64970.
DR   RefSeq; NP_416547.1; NC_000913.3.
DR   RefSeq; WP_001116026.1; NZ_LN832404.1.
DR   AlphaFoldDB; P71244; -.
DR   SMR; P71244; -.
DR   BioGRID; 4259677; 196.
DR   IntAct; P71244; 4.
DR   STRING; 511145.b2043; -.
DR   PaxDb; P71244; -.
DR   PRIDE; P71244; -.
DR   EnsemblBacteria; AAC75104; AAC75104; b2043.
DR   EnsemblBacteria; BAA15897; BAA15897; BAA15897.
DR   GeneID; 946561; -.
DR   KEGG; ecj:JW2028; -.
DR   KEGG; eco:b2043; -.
DR   PATRIC; fig|1411691.4.peg.208; -.
DR   EchoBASE; EB2521; -.
DR   eggNOG; ENOG502Z7QB; Bacteria.
DR   HOGENOM; CLU_046699_0_0_6; -.
DR   OMA; YDNDYPE; -.
DR   PhylomeDB; P71244; -.
DR   BioCyc; EcoCyc:G7094-MON; -.
DR   UniPathway; UPA00936; -.
DR   PRO; PR:P71244; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0045228; P:slime layer polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR023882; Colanic_acid_synth_WcaM.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   TIGRFAMs; TIGR04004; WcaM; 1.
PE   3: Inferred from homology;
KW   Lipopolysaccharide biosynthesis; Reference proteome; Signal.
FT   SIGNAL          1..28
FT                   /note="Tat-type signal"
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..464
FT                   /note="Colanic acid biosynthesis protein WcaM"
FT                   /id="PRO_0000065958"
SQ   SEQUENCE   464 AA;  51316 MW;  72A7655DC07368BE CRC64;
     MPFKKLSRRT FLTASSALAF LHTPFARALP ARQSVNINDY NPHDWIASFK QAFSEGQTVV
     VPAGLVCDNI NTGIFIPPGK TLHILGSLRG NGRGRFVLQD GSQVTGEDGG SMHNITLDVR
     GSDCTIKGLT MSGFGPVTQI YIGGKNKRVM RNLIIDNLTV SHANYAILRQ GFHNQIIGAN
     ITNCKFSDLQ GDAIEWNVAI NDRDILISDH VIERINCTNG KINWGIGIGL AGSTYDNNYP
     EDQAVKNFVV ANITGSDCRQ LIHVENGKHF VIRNIKARNI TPDFSKKAGI DNATVAIYGC
     DNFVIDNIEM INSAGMLIGY GVIKGKYLSI PQNFRVNDIQ LDNTHLAYKL RGIQISAGNA
     VSFVALTNIE MKRASLELHN KPQHFFMRNI NVMQESSVGP ALSMNFDMRK DVRGVFMAKK
     ETLLSLANVH AVNEKGQSSV DIDRINHHIV NVEKINFRLP ELRE