ID   WCAM_SHIFL              Reviewed;         464 AA.
AC   P37775;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2003, sequence version 2.
DT   25-MAY-2022, entry version 121.
DE   RecName: Full=Colanic acid biosynthesis protein WcaM;
GN   Name=wcaM; OrderedLocusNames=SF2106, S2229;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 156-464.
RC   STRAIN=PE577 / Serotype 2a;
RX   PubMed=7507920; DOI=10.1128/jb.176.3.733-747.1994;
RA   Morona R., Mavris M., Fallarino A., Manning P.A.;
RT   "Characterization of the rfc region of Shigella flexneri.";
RL   J. Bacteriol. 176:733-747(1994).
CC   -!- PATHWAY: Slime biogenesis; slime polysaccharide biosynthesis.
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DR   EMBL; AE005674; AAN43645.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP17474.1; -; Genomic_DNA.
DR   EMBL; X71970; CAA50765.1; -; Genomic_DNA.
DR   RefSeq; NP_707938.1; NC_004337.2.
DR   RefSeq; WP_001116126.1; NZ_WPGW01000076.1.
DR   AlphaFoldDB; P37775; -.
DR   SMR; P37775; -.
DR   STRING; 198214.SF2106; -.
DR   EnsemblBacteria; AAN43645; AAN43645; SF2106.
DR   EnsemblBacteria; AAP17474; AAP17474; S2229.
DR   GeneID; 1025865; -.
DR   KEGG; sfl:SF2106; -.
DR   KEGG; sfx:S2229; -.
DR   PATRIC; fig|198214.7.peg.2514; -.
DR   HOGENOM; CLU_046699_0_0_6; -.
DR   OMA; YDNDYPE; -.
DR   OrthoDB; 284041at2; -.
DR   UniPathway; UPA00936; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0045228; P:slime layer polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR023882; Colanic_acid_synth_WcaM.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   TIGRFAMs; TIGR04004; WcaM; 1.
PE   4: Predicted;
KW   Lipopolysaccharide biosynthesis; Reference proteome.
FT   CHAIN           1..464
FT                   /note="Colanic acid biosynthesis protein WcaM"
FT                   /id="PRO_0000065960"
FT   CONFLICT        395
FT                   /note="E -> D (in Ref. 3; CAA50765)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        398
FT                   /note="V -> L (in Ref. 3; CAA50765)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        455..464
FT                   /note="INFRLPERRE -> Y (in Ref. 3; CAA50765)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   464 AA;  51191 MW;  0EC3BA7001C7D43D CRC64;
     MPFKTLSRRT FLTASSALAF LHTPFARALP ARQSVNINDY NPHDWIASFK QAFSEGQTVV
     VPAGFVCDNI NTGIFIPPGK TLHILGSLRG NGRGRFVLQD GSQVTGGEGG GMHNITLDVR
     GSDCTIKGLA MSGFGPVMQI YIGGKNKRVM RNLTIDNLTV SHANYAILRQ GFHNQIIGAN
     ITNCKFSDLQ GDAIEWNVAI NDSDILISDH VIERINCTNG KINWGIGIGL AGSTYDNNYP
     EDQAVKNFVV ANITGSDCRQ LIHVENGKHF VIRNINARNI TPDFSKKAGI DNATVAIYGC
     DNFVIDNIEM INSAGMLIGY GVIKGKYLSI PQNFRVNNIQ LDNTHLAYKL RGIQISAGNA
     VSFVSLTNIE MKRASLELHN KPQHLFMRNI NVMQESSVGP ALSMNFDMRK DVRGVFMAKK
     ETLLSLANVH AVNERGQSSV DIDRINHHIV NVEKINFRLP ERRE