ID   WDR18_DANRE             Reviewed;         431 AA.
AC   Q68EI0;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=WD repeat-containing protein 18;
GN   Name=wdr18;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=21876750; DOI=10.1371/journal.pone.0023386;
RA   Gao W., Xu L., Guan R., Liu X., Han Y., Wu Q., Xiao Y., Qi F., Zhu Z.,
RA   Lin S., Zhang B.;
RT   "Wdr18 is required for Kupffer's vesicle formation and regulation of body
RT   asymmetry in zebrafish.";
RL   PLoS ONE 6:E23386-E23386(2011).
CC   -!- FUNCTION: Involved in left-right determination through controlling the
CC       correct clustering and migration of dorsal forerunner cells (DFCs) and
CC       Kupffer's vesicle formation (PubMed:21876750). Component of the PELP1
CC       complex involved in the nucleolar steps of 28S rRNA maturation and the
CC       subsequent nucleoplasmic transit of the pre-60S ribosomal subunit (By
CC       similarity). {ECO:0000250|UniProtKB:Q9BV38,
CC       ECO:0000269|PubMed:21876750}.
CC   -!- SUBUNIT: Component of the PELP1 complex, composed of at least PELP1,
CC       TEX10 and WDR18. The complex interacts with pre-60S ribosome particles.
CC       {ECO:0000250|UniProtKB:Q9BV38}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:Q9BV38}. Nucleus, nucleoplasm
CC       {ECO:0000250|UniProtKB:Q4VBE8}. Dynein axonemal particle
CC       {ECO:0000250|UniProtKB:A0A1L8HX76}.
CC   -!- DEVELOPMENTAL STAGE: Expressed from the 1-4 cell stage and in DFCs at
CC       75%-epiboly stage. By the 6-somite stage, its expression in the
CC       Kupffer's vesicle is detectable in addition to some basal expression
CC       levels in other cells. Later at the 18-somite stage, detected
CC       ubiquitously throughout the embryo. After 2 dpf (days post
CC       fertilization), expressed in endodermal organs, including the pancreas
CC       and liver. {ECO:0000269|PubMed:21876750}.
CC   -!- DISRUPTION PHENOTYPE: Affects the left-right asymmetry of internal
CC       organs, but not their specification. {ECO:0000269|PubMed:21876750}.
CC   -!- SIMILARITY: Belongs to the WD repeat IPI3/WDR18 family. {ECO:0000305}.
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DR   EMBL; BX640454; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CR555295; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC080252; AAH80252.1; -; mRNA.
DR   RefSeq; NP_001005206.1; NM_001005206.1.
DR   AlphaFoldDB; Q68EI0; -.
DR   SMR; Q68EI0; -.
DR   STRING; 7955.ENSDARP00000060250; -.
DR   PaxDb; Q68EI0; -.
DR   Ensembl; ENSDART00000060251; ENSDARP00000060250; ENSDARG00000041113.
DR   GeneID; 445819; -.
DR   KEGG; dre:445819; -.
DR   CTD; 57418; -.
DR   ZFIN; ZDB-GENE-040905-4; wdr18.
DR   eggNOG; KOG0646; Eukaryota.
DR   GeneTree; ENSGT00390000000289; -.
DR   HOGENOM; CLU_029749_0_0_1; -.
DR   InParanoid; Q68EI0; -.
DR   OMA; VNARIYT; -.
DR   OrthoDB; 747023at2759; -.
DR   PhylomeDB; Q68EI0; -.
DR   TreeFam; TF313046; -.
DR   Reactome; R-DRE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   PRO; PR:Q68EI0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 11.
DR   Bgee; ENSDARG00000041113; Expressed in blastula and 30 other tissues.
DR   ExpressionAtlas; Q68EI0; baseline.
DR   GO; GO:0120293; C:dynein axonemal particle; ISS:UniProtKB.
DR   GO; GO:0005656; C:nuclear pre-replicative complex; IBA:GO_Central.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0097344; C:Rix1 complex; IBA:GO_Central.
DR   GO; GO:0007368; P:determination of left/right symmetry; IMP:UniProtKB.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR   GO; GO:0070121; P:Kupffer's vesicle development; IMP:UniProtKB.
DR   GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR045227; WDR18/Ipi3/RID3.
DR   InterPro; IPR026987; Wdr18_C_dom.
DR   PANTHER; PTHR18763; PTHR18763; 1.
DR   Pfam; PF00400; WD40; 2.
DR   Pfam; PF14077; WD40_alt; 1.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 5.
DR   SUPFAM; SSF50998; SSF50998; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 3.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Nucleus; Reference proteome; Repeat;
KW   WD repeat.
FT   CHAIN           1..431
FT                   /note="WD repeat-containing protein 18"
FT                   /id="PRO_0000413755"
FT   REPEAT          78..118
FT                   /note="WD 1"
FT   REPEAT          119..158
FT                   /note="WD 2"
FT   REPEAT          170..211
FT                   /note="WD 3"
FT   REPEAT          213..252
FT                   /note="WD 4"
FT   REPEAT          267..306
FT                   /note="WD 5"
FT   REGION          342..363
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        345..363
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   431 AA;  47155 MW;  3653003A1B206357 CRC64;
     MSAPVEVVLS ADSAGQLFNC AVYDPHTGSE FLSYRGGNTS SRSLTILNGE YILGAQLGKN
     YINVWEIQRK DQLQQKIVCP GIVTCLCASP DGLYVLAGIA EAIYLWEVST GNLLAILSRH
     FQDLSCIKFT DDSSHFVSGG KDNLAFIWNL SSVVQLDSSR TPEPRHILSR HSLPITDIHC
     GLMGPQARVA TASLDQTVKV WEISSGEMLL SVLFDVGIMS VTFDPCEYFL FCGGSDGNIF
     QVSLCSTSLS RDKTFQSDSE GNQVFKGHRN LVTCLSVSMD GTVLLSGSND ETVRMWDVQS
     KQCIWTINHR GPVTNAAIIP APANMFLSDS HPAVPLPRFS RHLNPSEQGD GTGTGGMSLR
     LGANTQEPEG TYLEKAEELY SLMCAVTDKS VFGDGENTKV RVSELEEEVR TLKKINKDLY
     EFSTQLLTKP N