WDR19_MOUSE
ID WDR19_MOUSE Reviewed; 1341 AA.
AC Q3UGF1; Q6ZPK8; Q80VQ6; Q8C794; Q8K3R5;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=WD repeat-containing protein 19 {ECO:0000305};
DE AltName: Full=Intraflagellar transport 144 homolog;
GN Name=Wdr19 {ECO:0000312|MGI:MGI:2443231};
GN Synonyms=Ift144 {ECO:0000303|PubMed:25860617}, Kiaa1638;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ;
RX PubMed=12906858; DOI=10.1016/s0888-7543(03)00151-4;
RA Lin B., White J.T., Utleg A.G., Wang S., Ferguson C., True L.D.,
RA Vessella R., Hood L., Nelson P.S.;
RT "Isolation and characterization of human and mouse WDR19,a novel WD-repeat
RT protein exhibiting androgen-regulated expression in prostate epithelium.";
RL Genomics 82:331-342(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Heart, and Melanocyte;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 335-1239 (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [4]
RP SEQUENCE REVISION.
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 581-1341.
RC STRAIN=NMRI; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16957054; DOI=10.1091/mbc.e06-04-0260;
RA Efimenko E., Blacque O.E., Ou G., Haycraft C.J., Yoder B.K., Scholey J.M.,
RA Leroux M.R., Swoboda P.;
RT "Caenorhabditis elegans DYF-2, an orthologue of human WDR19, is a component
RT of the intraflagellar transport machinery in sensory cilia.";
RL Mol. Biol. Cell 17:4801-4811(2006).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=19208653; DOI=10.1093/hmg/ddp068;
RA Jiang S.T., Chiou Y.Y., Wang E., Chien Y.L., Ho H.H., Tsai F.J., Lin C.Y.,
RA Tsai S.P., Li H.;
RT "Essential role of nephrocystin in photoreceptor intraflagellar transport
RT in mouse.";
RL Hum. Mol. Genet. 18:1566-1577(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [9]
RP FUNCTION, INTERACTION WITH BBS1, AND MUTAGENESIS OF GLY-341.
RX PubMed=22922713; DOI=10.1038/ncb2560;
RA Wei Q., Zhang Y., Li Y., Zhang Q., Ling K., Hu J.;
RT "The BBSome controls IFT assembly and turnaround in cilia.";
RL Nat. Cell Biol. 14:950-957(2012).
RN [10]
RP INTERACTION WITH TTC25.
RX PubMed=25860617; DOI=10.1371/journal.pone.0124378;
RA Xu Y., Cao J., Huang S., Feng D., Zhang W., Zhu X., Yan X.;
RT "Characterization of tetratricopeptide repeat-containing proteins critical
RT for cilia formation and function.";
RL PLoS ONE 10:E0124378-E0124378(2015).
CC -!- FUNCTION: As component of the IFT complex A (IFT-A), a complex required
CC for retrograde ciliary transport and entry into cilia of G protein-
CC coupled receptors (GPCRs), it is involved in cilia function and/or
CC assembly (Probable). Essential for functional IFT-A assembly and
CC ciliary entry of GPCRs (By similarity). Associates with the BBSome
CC complex to mediate ciliary transport (PubMed:22922713).
CC {ECO:0000250|UniProtKB:Q8NEZ3, ECO:0000269|PubMed:22922713,
CC ECO:0000305|PubMed:16957054}.
CC -!- SUBUNIT: Component of the IFT complex A (IFT-A) complex. IFT-A complex
CC is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which
CC is associated with TULP3 and a peripheral subcomplex composed of
CC IFT43:WDR35:TTC21B (By similarity). Interacts (via C-terminal region)
CC with IFT122 (via C-terminal region) (By similarity). Interacts with
CC BBS1 (PubMed:22922713). Interacts with TTC25 (PubMed:25860617).
CC {ECO:0000250|UniProtKB:Q8NEZ3, ECO:0000269|PubMed:22922713,
CC ECO:0000269|PubMed:25860617}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:16957054}. Cytoplasm, cytoskeleton, cilium basal
CC body {ECO:0000269|PubMed:16957054}. Cell projection, cilium,
CC photoreceptor outer segment {ECO:0000269|PubMed:19208653}.
CC Note=Localizes to photoreceptor connecting cilia, to the base of motile
CC cilia in brain ependymal cells and to the base of and along primary
CC cilia in cultured kidney inner mnedullary collecting duct (IMCD) cells.
CC {ECO:0000269|PubMed:16957054, ECO:0000269|PubMed:19208653}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q3UGF1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UGF1-2; Sequence=VSP_018075;
CC Name=3;
CC IsoId=Q3UGF1-3; Sequence=VSP_018076, VSP_018077;
CC -!- TISSUE SPECIFICITY: Tissue-specific expression of isoforms
CC (PubMed:12906858). Expressed in the prostate, testis, epididymis,
CC submaxillary and salivary glands (PubMed:12906858). Expressed in
CC ependymal cells lining brain ventricles (at protein level)
CC (PubMed:16957054). {ECO:0000269|PubMed:12906858,
CC ECO:0000269|PubMed:16957054}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH46432.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY029258; AAK38746.1; -; mRNA.
DR EMBL; AK052303; BAC34929.1; -; mRNA.
DR EMBL; AK147970; BAE28258.1; -; mRNA.
DR EMBL; AK129413; BAC98223.2; -; Transcribed_RNA.
DR EMBL; BC046432; AAH46432.1; ALT_SEQ; mRNA.
DR CCDS; CCDS51509.1; -. [Q3UGF1-1]
DR RefSeq; NP_700440.2; NM_153391.2. [Q3UGF1-1]
DR RefSeq; XP_011239015.1; XM_011240713.1.
DR AlphaFoldDB; Q3UGF1; -.
DR SMR; Q3UGF1; -.
DR BioGRID; 229398; 4.
DR ComplexPortal; CPX-5027; IFT-A complex.
DR DIP; DIP-61859N; -.
DR IntAct; Q3UGF1; 1.
DR STRING; 10090.ENSMUSP00000038098; -.
DR iPTMnet; Q3UGF1; -.
DR PhosphoSitePlus; Q3UGF1; -.
DR MaxQB; Q3UGF1; -.
DR PaxDb; Q3UGF1; -.
DR PeptideAtlas; Q3UGF1; -.
DR PRIDE; Q3UGF1; -.
DR ProteomicsDB; 297637; -. [Q3UGF1-1]
DR ProteomicsDB; 297638; -. [Q3UGF1-2]
DR ProteomicsDB; 297639; -. [Q3UGF1-3]
DR Antibodypedia; 23402; 126 antibodies from 20 providers.
DR DNASU; 213081; -.
DR Ensembl; ENSMUST00000041892; ENSMUSP00000038098; ENSMUSG00000037890. [Q3UGF1-1]
DR Ensembl; ENSMUST00000203653; ENSMUSP00000144866; ENSMUSG00000037890. [Q3UGF1-1]
DR GeneID; 213081; -.
DR KEGG; mmu:213081; -.
DR UCSC; uc008xne.1; mouse. [Q3UGF1-1]
DR UCSC; uc008xnf.1; mouse. [Q3UGF1-3]
DR CTD; 57728; -.
DR MGI; MGI:2443231; Wdr19.
DR VEuPathDB; HostDB:ENSMUSG00000037890; -.
DR eggNOG; KOG2247; Eukaryota.
DR GeneTree; ENSGT00590000083165; -.
DR HOGENOM; CLU_003002_2_0_1; -.
DR InParanoid; Q3UGF1; -.
DR OMA; KNTIPFC; -.
DR OrthoDB; 215211at2759; -.
DR PhylomeDB; Q3UGF1; -.
DR TreeFam; TF314758; -.
DR Reactome; R-MMU-5610787; Hedgehog 'off' state.
DR Reactome; R-MMU-5620924; Intraflagellar transport.
DR BioGRID-ORCS; 213081; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Wdr19; mouse.
DR PRO; PR:Q3UGF1; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q3UGF1; protein.
DR Bgee; ENSMUSG00000037890; Expressed in spermatocyte and 196 other tissues.
DR ExpressionAtlas; Q3UGF1; baseline and differential.
DR GO; GO:0005929; C:cilium; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0030991; C:intraciliary transport particle A; IDA:MGI.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0097730; C:non-motile cilium; IDA:UniProtKB.
DR GO; GO:0032391; C:photoreceptor connecting cilium; IDA:UniProtKB.
DR GO; GO:0001750; C:photoreceptor outer segment; IEA:UniProtKB-SubCell.
DR GO; GO:0000902; P:cell morphogenesis; IMP:MGI.
DR GO; GO:0060830; P:ciliary receptor clustering involved in smoothened signaling pathway; IGI:MGI.
DR GO; GO:0060271; P:cilium assembly; IMP:MGI.
DR GO; GO:0055123; P:digestive system development; IMP:MGI.
DR GO; GO:0042471; P:ear morphogenesis; IMP:MGI.
DR GO; GO:0031076; P:embryonic camera-type eye development; IMP:MGI.
DR GO; GO:0048701; P:embryonic cranial skeleton morphogenesis; IMP:MGI.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:MGI.
DR GO; GO:0008406; P:gonad development; IMP:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0035721; P:intraciliary retrograde transport; ISO:MGI.
DR GO; GO:0061055; P:myotome development; IMP:MGI.
DR GO; GO:0050877; P:nervous system process; IMP:MGI.
DR GO; GO:1903441; P:protein localization to ciliary membrane; ISS:UniProtKB.
DR GO; GO:0061512; P:protein localization to cilium; ISO:MGI.
DR GO; GO:0065003; P:protein-containing complex assembly; ISS:UniProtKB.
DR GO; GO:0007224; P:smoothened signaling pathway; IMP:MGI.
DR GO; GO:0060831; P:smoothened signaling pathway involved in dorsal/ventral neural tube patterning; IMP:MGI.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR040379; WDR19/dyf-2.
DR InterPro; IPR039468; WDR19_WD40_rpt.
DR PANTHER; PTHR14920; PTHR14920; 1.
DR Pfam; PF15911; WD40_3; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Cytoplasm; Cytoskeleton; Reference proteome;
KW Repeat; TPR repeat; WD repeat.
FT CHAIN 1..1341
FT /note="WD repeat-containing protein 19"
FT /id="PRO_0000233157"
FT REPEAT 11..51
FT /note="WD 1"
FT REPEAT 52..92
FT /note="WD 2"
FT REPEAT 95..134
FT /note="WD 3"
FT REPEAT 137..175
FT /note="WD 4"
FT REPEAT 273..311
FT /note="WD 5"
FT REPEAT 317..356
FT /note="WD 6"
FT REPEAT 736..769
FT /note="TPR 1"
FT REPEAT 775..808
FT /note="TPR 2"
FT REPEAT 840..873
FT /note="TPR 3"
FT REPEAT 895..928
FT /note="TPR 4"
FT REPEAT 951..984
FT /note="TPR 5"
FT REPEAT 1020..1053
FT /note="TPR 6"
FT VAR_SEQ 1..59
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_018075"
FT VAR_SEQ 1002..1021
FT /note="AEDTTNEDYQSIALYFEGEK -> KCCYLPYFLINMCYHRNPDS (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_018076"
FT VAR_SEQ 1022..1341
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_018077"
FT MUTAGEN 341
FT /note="G->R: Reduced interaction with BBS1."
FT /evidence="ECO:0000269|PubMed:22922713"
FT CONFLICT 1231..1232
FT /note="KK -> RE (in Ref. 1; AAK38746)"
FT /evidence="ECO:0000305"
FT CONFLICT 1257
FT /note="Q -> K (in Ref. 1; AAK38746)"
FT /evidence="ECO:0000305"
FT CONFLICT 1261
FT /note="P -> S (in Ref. 1; AAK38746)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1341 AA; 151457 MW; D0658DA05745B7CF CRC64;
MKRVFSLLEK SWLGAPIQFA WQKSSGNYLA VTGADYIVKI FDRHGQKRSE ISLPGNCVTM
DWDKDGDILA VIAEKSSCIY LWDANTNKTS QLDNGMRDQM SFLLWSKIGS FLAVGTIKGN
LLIYNHQTSR KIPVLGKHTK KITCGCWNSE NLLALGGEDK MITVSNQEGD TIRQTPVKSE
PSDIKFSMSK TDERISSAEN TISAVVGKKM LFLFHLNEPD NPVDLEFQQA YGNIVCYSWY
GDGYIMIGFS RGTFLAISTH FPEVGQEIFK ARDHKDNLTS VALSQTLNKA ATCGDNCIKI
HDLTELRDMY AIINLDDENK GLGTLSWTDD GQLLALSTQR GSLHVFLTKL PILGDACHTR
IAYLTSLLEV TVANLIEGEP PITVSVDVEP TFVAVGLYHL AVGMNNRAWF YVLGENVVKK
LKDVEYLGTV ASICLHSDYA AALFEGKIQL HLIENEMLDA QEERETRLFP AVDDKCRILC
HALTSDFLIY GTDTGIIHYF FIEDWQFVND YRHPVGVKKL FPDPNGTRLV FIDEKSDGFV
YCPVNDATYE IPDFSPTIKG VLWENWPMDK GVFIAYDDDK VYTYAFHKDT IQGSKVILAG
STKLPFSHKP LLLYNGELTC QTQSGKINSI YLSTHSFLGS MKDTEPTDLR QMLTQTLLLK
RFSDAWDICK MLNDRTSWSE LAKACLHHME VEFAIRVSRT MGDVGTVMSL EQIKGIEDYN
LLAGHLAMFT NDFNLAQDLY LASNCPVAAL EMRRDLQHWD SALQLAKRLA PDQIPFISKE
YAIQLEFTGD YVNALAHYEK GITGDNKEHD EVCLAGVAQM SIRMGDIRRG ANQALKHPSR
VLKRDCGAIL ENMKQFSEAA QLYEKGQYYD RAASVYIRCK NWAKVGELLP HVSSPKIHLQ
YAKAKEADGR YKEAVVAYEN AKQWNSVIRI YLDHLNNPEK AVSIVRETQS LDGAKMVARF
FLQLGDYGSA IQFLVLSKCN NEAFTLAQQH NKMEIYADII GAEDTTNEDY QSIALYFEGE
KRHFQAGKFF LLCGQYSRAL KHFLKCPSSE DNVAIEMAIE TVGQAKDELL TNQLIDHLMG
ESDGMPKDAK YLFRLYMALK QYREAARTAI IIAREEQSAG NYRNAHDVLF SMYAELKAQK
IKIPSEMATN LMILHSYILV KIHVKSGDHM KGARMLIRVA NNISKFPSHI VPILTSTVIE
CHRAGLKNSA FSFAAMLMRP EYRNKIDAKY KKKIEAMVRR PDTSETEEAT TPCPFCQFLL
PECELLCPGC KNNIPYCIAT GRHMLKDDWT MCPHCGFPAL YSEFKILLNS ESTCPMCSER
LNSSQLKKIT DCSQYLRTEM E
