WDR35_MOUSE
ID WDR35_MOUSE Reviewed; 1181 AA.
AC Q8BND3; E9QLW3; Q8CDL4;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=WD repeat-containing protein 35 {ECO:0000305};
GN Name=Wdr35 {ECO:0000312|MGI:MGI:1921932};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Spinal ganglion, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21473986; DOI=10.1016/j.ajhg.2011.03.015;
RA Mill P., Lockhart P.J., Fitzpatrick E., Mountford H.S., Hall E.A.,
RA Reijns M.A., Keighren M., Bahlo M., Bromhead C.J., Budd P., Aftimos S.,
RA Delatycki M.B., Savarirayan R., Jackson I.J., Amor D.J.;
RT "Human and mouse mutations in WDR35 cause short-rib polydactyly syndromes
RT due to abnormal ciliogenesis.";
RL Am. J. Hum. Genet. 88:508-515(2011).
CC -!- FUNCTION: As a component of the IFT complex A (IFT-A), a complex
CC required for retrograde ciliary transport and entry into cilia of G
CC protein-coupled receptors (GPCRs), it is involved in ciliogenesis and
CC ciliary protein trafficking (PubMed:21473986). May promote CASP3
CC activation and TNF-stimulated apoptosis (By similarity).
CC {ECO:0000250|UniProtKB:Q9P2L0, ECO:0000269|PubMed:21473986}.
CC -!- SUBUNIT: Component of the IFT complex A (IFT-A) complex. IFT-A complex
CC is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which
CC is associated with TULP3 and a peripheral subcomplex composed of
CC IFT43:WDR35:TTC21B. Interacts directy with IFT122, ITF43 and TTC21B.
CC Interacts with IFT43. {ECO:0000250|UniProtKB:Q9P2L0}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:21473986}. Cytoplasm,
CC cytoskeleton, cilium axoneme {ECO:0000269|PubMed:21473986}. Cytoplasm,
CC cytoskeleton, cilium basal body {ECO:0000269|PubMed:21473986}.
CC Note=Immunofluorescence studies in IMCD3 cells show that Wdr35
CC accumulates at and around centrosomes and basal bodies of serum-starved
CC cells, with fainter punctate staining along the cilia axoneme.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BND3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BND3-2; Sequence=VSP_009733;
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DR EMBL; AK029896; BAC26663.1; -; mRNA.
DR EMBL; AK083971; BAC39081.1; -; mRNA.
DR EMBL; AC110376; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC056925; AAH56925.1; -; mRNA.
DR CCDS; CCDS25807.1; -. [Q8BND3-1]
DR CCDS; CCDS49027.1; -. [Q8BND3-2]
DR RefSeq; NP_001152999.1; NM_001159527.1. [Q8BND3-2]
DR RefSeq; NP_766058.3; NM_172470.3. [Q8BND3-1]
DR AlphaFoldDB; Q8BND3; -.
DR SMR; Q8BND3; -.
DR BioGRID; 216937; 3.
DR ComplexPortal; CPX-5027; IFT-A complex.
DR IntAct; Q8BND3; 1.
DR MINT; Q8BND3; -.
DR STRING; 10090.ENSMUSP00000082895; -.
DR iPTMnet; Q8BND3; -.
DR PhosphoSitePlus; Q8BND3; -.
DR MaxQB; Q8BND3; -.
DR PaxDb; Q8BND3; -.
DR PeptideAtlas; Q8BND3; -.
DR PRIDE; Q8BND3; -.
DR ProteomicsDB; 297644; -. [Q8BND3-1]
DR ProteomicsDB; 297645; -. [Q8BND3-2]
DR Antibodypedia; 50378; 65 antibodies from 22 providers.
DR DNASU; 74682; -.
DR Ensembl; ENSMUST00000085745; ENSMUSP00000082895; ENSMUSG00000066643. [Q8BND3-1]
DR Ensembl; ENSMUST00000111113; ENSMUSP00000106742; ENSMUSG00000066643. [Q8BND3-2]
DR GeneID; 74682; -.
DR KEGG; mmu:74682; -.
DR UCSC; uc007naj.2; mouse. [Q8BND3-1]
DR UCSC; uc007nak.2; mouse. [Q8BND3-2]
DR CTD; 57539; -.
DR MGI; MGI:1921932; Wdr35.
DR VEuPathDB; HostDB:ENSMUSG00000066643; -.
DR eggNOG; KOG2041; Eukaryota.
DR GeneTree; ENSGT00940000155745; -.
DR HOGENOM; CLU_004048_1_0_1; -.
DR InParanoid; Q8BND3; -.
DR OMA; HCASPME; -.
DR OrthoDB; 95796at2759; -.
DR PhylomeDB; Q8BND3; -.
DR TreeFam; TF314076; -.
DR Reactome; R-MMU-5610787; Hedgehog 'off' state.
DR Reactome; R-MMU-5620924; Intraflagellar transport.
DR BioGRID-ORCS; 74682; 2 hits in 70 CRISPR screens.
DR ChiTaRS; Wdr35; mouse.
DR PRO; PR:Q8BND3; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8BND3; protein.
DR Bgee; ENSMUSG00000066643; Expressed in spermatocyte and 209 other tissues.
DR ExpressionAtlas; Q8BND3; baseline and differential.
DR Genevisible; Q8BND3; MM.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR GO; GO:0005929; C:cilium; IC:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0030991; C:intraciliary transport particle A; IDA:MGI.
DR GO; GO:1990830; P:cellular response to leukemia inhibitory factor; IEP:MGI.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; ISO:MGI.
DR GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR GO; GO:0035721; P:intraciliary retrograde transport; ISO:MGI.
DR GO; GO:0042073; P:intraciliary transport; ISO:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:MGI.
DR GO; GO:0045019; P:negative regulation of nitric oxide biosynthetic process; ISO:MGI.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISO:MGI.
DR GO; GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; ISO:MGI.
DR GO; GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; ISO:MGI.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; ISO:MGI.
DR GO; GO:0061512; P:protein localization to cilium; ISO:MGI.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR017233; WDR35.
DR PANTHER; PTHR16517:SF1; PTHR16517:SF1; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR PIRSF; PIRSF037536; WD_repeat_p35; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Cytoplasm; Cytoskeleton; Reference proteome;
KW Repeat; WD repeat.
FT CHAIN 1..1181
FT /note="WD repeat-containing protein 35"
FT /id="PRO_0000051385"
FT REPEAT 12..51
FT /note="WD 1"
FT REPEAT 69..108
FT /note="WD 2"
FT REPEAT 113..152
FT /note="WD 3"
FT REPEAT 154..193
FT /note="WD 4"
FT REPEAT 502..539
FT /note="WD 5"
FT VAR_SEQ 399..409
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_009733"
FT CONFLICT 634
FT /note="Y -> H (in Ref. 3; AAH56925)"
FT /evidence="ECO:0000305"
FT CONFLICT 828
FT /note="N -> D (in Ref. 1; BAC39081)"
FT /evidence="ECO:0000305"
FT CONFLICT 915
FT /note="S -> Y (in Ref. 1; BAC26663)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1181 AA; 133991 MW; F69BA836E9CA65CE CRC64;
MFFYLSKKIA VPNNVKLKCI SWNKDQGFIA CGGEDGLLKV LRLETQTDDS KLRGLAAPSN
LSMNQNLEGH SGAVQVVTWN EQYQKLTTSD QNGLIIVWML YKGSWYEEMI NNRNKSVVRS
MSWNADGQKI CIVYEDGAVI VGSVDGNRIW GKDLKGIQLC HVTWSADSKI LLFGMANGEI
HIYDNQGNFI MKMKLNCLVN VTGAISIAGI HWYHGTEGYV EPDCPCLAIC FDNGRCQIMR
HENDQNPVLI DTGMYVVGIQ WNHIGSVLAV AGSQKVVTQD KDINIVQFYT PFGEHLGTLK
VPGKQMCSLS WEGGGLKIAL AVDSFIYFAN IRPDYKWGYC SNTVVYAYTR PDRPEYCVVF
WDTKNSEKYV KYVKSLISIT TCGDFCILAT KADENHPQEE NEMETFGATF VLVLCNSIGT
PLDPKYIDLV PLFVAMTKTH VIAASKEAFY TWQYRVAKKL TALEINQITR SRKEGRERIY
HVDDVPSGSV DGVFDYSKAI QGTRDPICAI TASDKTLIVG RESGVIQRYS FPNVALIQKY
SLDCRACQLS LNCNSSRLAI IDIAGVLTFF DLDTRVTDST GQQVVGELLK LERKDVWDMK
WAKDNPDLFA MMEKTRMYVF RNLDPEEPIQ TSGYICNFED LEIKSVLLDE ILKDPEHPSK
DYIMNFEIRS LRDSRALIEK VGIEDASQFI EDNPHPRLWR LLAEAALQKL DLYTAQQAFV
RCKDYQGIKF VKLLGNLQSE SMKQAEVIAY FGRFEDAERM YQDMDRRDLA IGLRMKLGDW
FRVLQLLKTG SGDADDSLLE QANNAIGEYF ADRQKWQNAV QYYVKGRNQE RLAECYYMLE
DYEGLETLAN SLPENHKLLP EIAQMFVRVG MCEQAVSAFL KCNQPKAAVD TCVHLNQWNK
AVELAKSHSM KEIGSLLARY ASHLLEKNKT LDAIELYRKA SYFFDAAKLM YKIADEEAKK
RTKPLRVKKL YVLSALLIEQ YHEQMKNAQR GKVKGKNSEA TSALAGLLEE EVLSTTSRFT
DNAWRGAEAY HFFILAQRQL YEGYVDTALK TALHLRDYED IIPSVEIYSL LALCACASRA
FGTCSKAFIK LESLETLSAE QKQQYEDLAL EIFTKHTPKD NRKSELNSLL EGGEGKLPTC
IATGSPIIEY QFWVCKVCKH YVLAQEISNY NFCPLCHSSV E
