ID   WDR35_RAT               Reviewed;        1170 AA.
AC   A6N6J5;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=WD repeat-containing protein 35;
DE   AltName: Full=Naofen;
GN   Name=Wdr35;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC   STRAIN=Wistar;
RX   PubMed=20193664; DOI=10.1016/j.bbrc.2010.02.133;
RA   Feng G.G., Li C., Huang L., Tsunekawa K., Sato Y., Fujiwara Y., Komatsu T.,
RA   Honda T., Fan J.H., Goto H., Koide T., Hasegawa T., Ishikawa N.;
RT   "Naofen, a novel WD40-repeat protein, mediates spontaneous and tumor
RT   necrosis factor-induced apoptosis.";
RL   Biochem. Biophys. Res. Commun. 394:153-157(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: As a component of the IFT complex A (IFT-A), a complex
CC       required for retrograde ciliary transport and entry into cilia of G
CC       protein-coupled receptors (GPCRs), it is involved in ciliogenesis and
CC       ciliary protein trafficking (By similarity). May promote CASP3
CC       activation and TNF-stimulated apoptosis (PubMed:20193664).
CC       {ECO:0000250|UniProtKB:Q9P2L0, ECO:0000269|PubMed:20193664}.
CC   -!- SUBUNIT: Component of the IFT complex A (IFT-A) complex. IFT-A complex
CC       is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which
CC       is associated with TULP3 and a peripheral subcomplex composed of
CC       IFT43:WDR35:TTC21B. Interacts directy with IFT122, ITF43 and TTC21B.
CC       Interacts with IFT43. {ECO:0000250|UniProtKB:Q9P2L0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome {ECO:0000250|UniProtKB:Q8BND3}. Cytoplasm,
CC       cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q8BND3}. Cytoplasm,
CC       cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q8BND3}.
CC   -!- TISSUE SPECIFICITY: Expressed at high levels in testis and at lower
CC       levels in the brain (at protein level). Also present in other tissues,
CC       including heart, uterus, spinal cord, ovary, liver, kidney, lung,
CC       pancreas and stomach. {ECO:0000269|PubMed:20193664}.
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DR   EMBL; EF613262; ABR22620.1; -; mRNA.
DR   EMBL; CH473947; EDM03084.1; -; Genomic_DNA.
DR   RefSeq; NP_001092810.1; NM_001099340.1.
DR   AlphaFoldDB; A6N6J5; -.
DR   SMR; A6N6J5; -.
DR   STRING; 10116.ENSRNOP00000042019; -.
DR   PaxDb; A6N6J5; -.
DR   PeptideAtlas; A6N6J5; -.
DR   PRIDE; A6N6J5; -.
DR   Ensembl; ENSRNOT00000051970; ENSRNOP00000042019; ENSRNOG00000028783.
DR   GeneID; 503018; -.
DR   KEGG; rno:503018; -.
DR   CTD; 57539; -.
DR   RGD; 1564116; Wdr35.
DR   eggNOG; KOG2041; Eukaryota.
DR   GeneTree; ENSGT00940000155745; -.
DR   HOGENOM; CLU_004048_1_0_1; -.
DR   InParanoid; A6N6J5; -.
DR   OMA; HCASPME; -.
DR   OrthoDB; 95796at2759; -.
DR   PhylomeDB; A6N6J5; -.
DR   Reactome; R-RNO-5610787; Hedgehog 'off' state.
DR   Reactome; R-RNO-5620924; Intraflagellar transport.
DR   PRO; PR:A6N6J5; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Proteomes; UP000234681; Chromosome 6.
DR   Bgee; ENSRNOG00000028783; Expressed in testis and 18 other tissues.
DR   Genevisible; A6N6J5; RN.
DR   GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR   GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR   GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR   GO; GO:0030991; C:intraciliary transport particle A; ISS:UniProtKB.
DR   GO; GO:0071333; P:cellular response to glucose stimulus; IEP:RGD.
DR   GO; GO:1990830; P:cellular response to leukemia inhibitory factor; ISO:RGD.
DR   GO; GO:1905705; P:cellular response to paclitaxel; IEP:RGD.
DR   GO; GO:0071356; P:cellular response to tumor necrosis factor; IMP:RGD.
DR   GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR   GO; GO:0035721; P:intraciliary retrograde transport; ISO:RGD.
DR   GO; GO:0042073; P:intraciliary transport; ISO:RGD.
DR   GO; GO:0097421; P:liver regeneration; IEP:RGD.
DR   GO; GO:0010629; P:negative regulation of gene expression; IDA:RGD.
DR   GO; GO:0045019; P:negative regulation of nitric oxide biosynthetic process; IDA:RGD.
DR   GO; GO:0043065; P:positive regulation of apoptotic process; IMP:RGD.
DR   GO; GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IMP:RGD.
DR   GO; GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; IMP:RGD.
DR   GO; GO:0045907; P:positive regulation of vasoconstriction; IDA:RGD.
DR   GO; GO:0061512; P:protein localization to cilium; ISO:RGD.
DR   GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR   GO; GO:0009636; P:response to toxic substance; IEP:RGD.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR024977; Apc4_WD40_dom.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR017233; WDR35.
DR   PANTHER; PTHR16517:SF1; PTHR16517:SF1; 1.
DR   Pfam; PF12894; ANAPC4_WD40; 1.
DR   PIRSF; PIRSF037536; WD_repeat_p35; 1.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   Cell projection; Cilium; Cilium biogenesis/degradation; Cytoplasm;
KW   Cytoskeleton; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..1170
FT                   /note="WD repeat-containing protein 35"
FT                   /id="PRO_0000405406"
FT   REPEAT          12..51
FT                   /note="WD 1"
FT   REPEAT          69..108
FT                   /note="WD 2"
FT   REPEAT          113..152
FT                   /note="WD 3"
FT   REPEAT          154..193
FT                   /note="WD 4"
FT   REPEAT          491..528
FT                   /note="WD 5"
SQ   SEQUENCE   1170 AA;  132779 MW;  70792ECD36111DEF CRC64;
     MFFYLSKKIA VPNNVKLKCI SWNKDQGFIA CGGEDGLLKV LRLETQTDDS KLRGLAAPSN
     LSMNQNLEGH SGAVQVVTWN EQYQKLTTSD QNGLIIVWML YKGSWYEEMI NNRNKSVVRS
     MSWNADGQKI CIVYEDGAVI VGSVDGNRIW GKDLKGIQLC HVTWSADSKI LLFGMANGEI
     HIYDNQGNFI MKMKLNCLVN VTGAISIAGI HWYHGTEGYV EPDCPCLAIC FDNGRCQIMR
     HENDQNPVLI DTGMYVVGIQ WNHIGSVLAV AGSQKVVTQD KDVNIVQFYT PFGEHLGTLK
     VPGKQMCSLS WEGGGLKIAL AVDSFIYFAN IRPDYKWGYC SNTVVYAYTR PDRPEYCVVF
     WDTKNNEKYV KYVKSLISIT TCGDFCILAT KADENHPQFV LVLCNSIGTP LDPKYIDLVP
     LFVAMTKTHV IAASKEALYT WQYRVAKKLT ALEINQITRS RKEGRERIYH VDDVPSGSVD
     GVFDYSKAIQ GTRDPICAIT ASDKTLIVGR ESGVIQRYSF PNVALIQKYS LDCRACQLSL
     NCNSSRLAII DIAGVLTFFD LDTRVTDSTG QQVVGELLKL ERKDVWDMKW AKDNPDLFAM
     MEKTRMYVFR NLDPEEPIQT SGYICNFEDL EIKSVLLDEI LKNPEHPSKD YIMNFEIRSL
     RDSRALIEKV GIEDASQFIE DNPHPRLWRL LAEAALQKLD LYTAQQAFVR CKDYQGIKFV
     KRLGNLQSES MKQAEVIAYF GRFEEAERMY LDMDRRDLAI GLRLKLGDWF RVLQLLKTGS
     GDADDSLLEQ AHNAIGDYFA DRQKWMNAVQ YYVKGRNQER LAECYYMLED YEGLENLANS
     LPENHKLLPE IAQMFVRVGM CEQAVSAFLK CNQPKAAVDT CVHLNQWNKA VELAKSHSMK
     EIGSLLARYA SHLLEKNKTL DAIELYRKAS YFFDAAKLMY KIADEEAKKR TKPLRVKKLY
     VLSALLIEQY HEQMKNAQRG KVKGKNSEAT SALAGLLEEE VLSTTSRFTD NAWRGAEAYH
     FFILAQRQLY EGYVDTALKT ALHLRDYEDI IPSVEIYSLL ALCACASRAF GTCSKAFIKL
     ESLETLSAEQ KQQYEDLALE IFTKHTPKDN RKSELNSLLE GGEGKLPTCI ATGSPIIEYQ
     FWVCKVCKHY VLAQEISNYN FCPLCHSSVE