ID   WDR44_RAT               Reviewed;         908 AA.
AC   Q9R037;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=WD repeat-containing protein 44;
DE   AltName: Full=Rabphilin-11;
GN   Name=Wdr44; Synonyms=RPH11;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 53-75; 194-216; 701-709;
RP   272-289; 294-306; 488-496; 537-546; 667-688; 740-751; 786-803; 832-856 AND
RP   882-895, FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Brain;
RX   PubMed=10464283; DOI=10.1074/jbc.274.36.25517;
RA   Mammoto A., Ohtsuka T., Hotta I., Sasaki T., Takai Y.;
RT   "Rab11BP/Rabphilin-11, a downstream target of rab11 small G protein
RT   implicated in vesicle recycling.";
RL   J. Biol. Chem. 274:25517-25524(1999).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40; THR-396; SER-398;
RP   SER-465; SER-466; SER-467 AND SER-556, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Downstream effector for RAB11. May be involved in vesicle
CC       recycling. {ECO:0000269|PubMed:10464283}.
CC   -!- SUBUNIT: Interacts with the GTP-bound form of RAB11 when membrane-
CC       associated. Does not bind to other Rab and Rho small G proteins (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:10464283}.
CC       Cytoplasm, perinuclear region {ECO:0000269|PubMed:10464283}. Endosome
CC       membrane {ECO:0000269|PubMed:10464283}. Golgi apparatus, trans-Golgi
CC       network {ECO:0000269|PubMed:10464283}. Note=Colocalized with RAB11
CC       along microtubules oriented toward lamellipodia.
CC   -!- TISSUE SPECIFICITY: Expressed in heart; brain; spleen; lung; liver;
CC       muscle and kidney. {ECO:0000269|PubMed:10464283}.
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DR   EMBL; AF130121; AAF02478.1; -; mRNA.
DR   AlphaFoldDB; Q9R037; -.
DR   SMR; Q9R037; -.
DR   STRING; 10116.ENSRNOP00000047275; -.
DR   iPTMnet; Q9R037; -.
DR   PhosphoSitePlus; Q9R037; -.
DR   jPOST; Q9R037; -.
DR   PaxDb; Q9R037; -.
DR   UCSC; RGD:727965; rat.
DR   RGD; 727965; Wdr44.
DR   eggNOG; KOG0283; Eukaryota.
DR   InParanoid; Q9R037; -.
DR   PhylomeDB; Q9R037; -.
DR   PRO; PR:Q9R037; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005874; C:microtubule; IDA:RGD.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:RGD.
DR   GO; GO:0031267; F:small GTPase binding; IDA:RGD.
DR   GO; GO:0030334; P:regulation of cell migration; IMP:RGD.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR040324; WDR44/Dgr2.
DR   PANTHER; PTHR14221; PTHR14221; 1.
DR   Pfam; PF00400; WD40; 4.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Endosome; Golgi apparatus; Membrane;
KW   Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..908
FT                   /note="WD repeat-containing protein 44"
FT                   /id="PRO_0000262771"
FT   REPEAT          504..543
FT                   /note="WD 1"
FT   REPEAT          600..638
FT                   /note="WD 2"
FT   REPEAT          640..680
FT                   /note="WD 3"
FT   REPEAT          685..724
FT                   /note="WD 4"
FT   REPEAT          735..774
FT                   /note="WD 5"
FT   REPEAT          779..818
FT                   /note="WD 6"
FT   REGION          1..163
FT                   /note="Binding activity"
FT   REGION          108..158
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          202..273
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          309..341
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          391..418
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          454..474
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          552..587
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        223..247
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        248..266
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        391..408
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        552..574
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         17
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         40
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         61
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         71
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         86
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         116
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         151
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         211
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         254
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         263
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         334
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         336
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         341
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
FT   MOD_RES         396
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         398
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         465
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         466
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         467
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         474
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6NVE8"
FT   MOD_RES         556
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         560
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5JSH3"
SQ   SEQUENCE   908 AA;  100948 MW;  E5F45471D10DD2CA CRC64;
     MMPLKMCTWG GSYPVGSPGK VGLLSFKETK NTGNKAGNES PVQELRQDVS KKIIECIIEE
     SQKVLQLEDD SLDSKGKGLS DQATASHSVA GTEFSNIPGL LAIQHELQQG SRKADSQNAA
     EETELETQKC FPSDNNCEKS EKTEDETNNL TEVSSADQLD ASKLETEILN KEAVEVKEGD
     VVNPASSDAL STKDFAAVEE VAPAKPPRHL TPEPDIVAST KKPVPARPPP PTNFPPPRPP
     PPSRPAPPPR KKKSDLEFEA LKTPDLDVPK DNIASDSLLT TNMASESTVR DSLPSLDLAS
     ATSGDKIVTA QENGKAPDVQ TVAGEVMGPQ RPRSNSGREL TDEEILASVM IKNLDTGEEI
     PLSLAEEKLP TGINPLTPLT LHIMRRTKEY VSNDATQSDD EEKLQSQQTD TDGGRLKQKT
     TQLKKFLGKS VKRAKHLAEE YGERAINKVK SVRDEVFHTD QDDPSSSDDE GMPYTRPVKF
     KAAHGFKGPY DFDQIKVVQD LSGEHMGAVW TMKFSHCGRL LASAGQDNIV RIWALKNAFD
     YFNNMRMKYN TEGRVSPSPS QESLSSSKSD TDMGVCSGTD EDPDDKNAPF RQRPFCKYKG
     HTADLLDLSW SKNYFLLSSS MDKTVRLWHI SRRECLCCFQ HIDFVTAIAF HPRDDRYFLS
     GSLDGKLRLW NIPDKKVALW NEVDGQTKLI TAANFCQNGK YAVIGTYDGR CIFYDTEHLK
     YHTQIHVRST RGRNKVGRKI TGIEPLPGEN KILVTSNDSR IRLYDLRDLS LSMKYKGYVN
     SSSQIKASFS HDFTYLVSGS EDKYVYIWST YHDLSKFTSV RRDRNDFWEG IKAHNAVVTS
     AIFAPNPSLM LSLDVQSEKL EGIDKYEDAE VLDNTSTGIV KTDNTEVLLS ADFTGAQCFY
     VKNSFLYP