WDR47_MOUSE
ID WDR47_MOUSE Reviewed; 920 AA.
AC Q8CGF6; A2AGC7; Q80TP4;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=WD repeat-containing protein 47;
DE AltName: Full=Neuronal enriched MAP interacting protein;
DE Short=Nemitin;
GN Name=Wdr47; Synonyms=Kiaa0893;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-285; SER-289 AND SER-292, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP INTERACTION WITH MAP1S, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=22523538; DOI=10.1371/journal.pone.0033094;
RA Wang W., Lundin V.F., Millan I., Zeng A., Chen X., Yang J., Allen E.,
RA Chen N., Bach G., Hsu A., Maloney M.T., Kapur M., Yang Y.;
RT "Nemitin, a novel Map8/Map1s interacting protein with Wd40 repeats.";
RL PLoS ONE 7:E33094-E33094(2012).
CC -!- SUBUNIT: Interacts with MAP1S (via WD repeats).
CC {ECO:0000269|PubMed:22523538}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:22523538}. Note=Localization along microtubules is
CC mediated by MAP1S.
CC -!- TISSUE SPECIFICITY: Enriched in the nervous system (at protein level).
CC {ECO:0000269|PubMed:22523538}.
CC -!- DEVELOPMENTAL STAGE: First detected in whole embryos at 11 dpc, strong
CC and increasing protein levels are observed in the brain from 14 dpc to
CC early postnatal development. Expression continues into adulthood,
CC though at a substantially decreased level.
CC {ECO:0000269|PubMed:22523538}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65679.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK122397; BAC65679.1; ALT_INIT; mRNA.
DR EMBL; AL683823; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466607; EDL01977.1; -; Genomic_DNA.
DR EMBL; BC040337; AAH40337.1; -; mRNA.
DR CCDS; CCDS17765.1; -.
DR RefSeq; NP_852065.2; NM_181400.3.
DR RefSeq; XP_017175308.1; XM_017319819.1.
DR AlphaFoldDB; Q8CGF6; -.
DR SMR; Q8CGF6; -.
DR BioGRID; 221273; 4.
DR DIP; DIP-58945N; -.
DR IntAct; Q8CGF6; 5.
DR STRING; 10090.ENSMUSP00000057482; -.
DR iPTMnet; Q8CGF6; -.
DR PhosphoSitePlus; Q8CGF6; -.
DR SwissPalm; Q8CGF6; -.
DR EPD; Q8CGF6; -.
DR jPOST; Q8CGF6; -.
DR MaxQB; Q8CGF6; -.
DR PaxDb; Q8CGF6; -.
DR PeptideAtlas; Q8CGF6; -.
DR PRIDE; Q8CGF6; -.
DR ProteomicsDB; 299972; -.
DR Antibodypedia; 33741; 50 antibodies from 14 providers.
DR DNASU; 99512; -.
DR Ensembl; ENSMUST00000051145; ENSMUSP00000057482; ENSMUSG00000040389.
DR GeneID; 99512; -.
DR KEGG; mmu:99512; -.
DR UCSC; uc008qzj.2; mouse.
DR CTD; 22911; -.
DR MGI; MGI:2139593; Wdr47.
DR VEuPathDB; HostDB:ENSMUSG00000040389; -.
DR eggNOG; KOG0641; Eukaryota.
DR GeneTree; ENSGT00940000155561; -.
DR HOGENOM; CLU_014985_0_0_1; -.
DR InParanoid; Q8CGF6; -.
DR OMA; NVGMENI; -.
DR PhylomeDB; Q8CGF6; -.
DR TreeFam; TF312810; -.
DR BioGRID-ORCS; 99512; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Wdr47; mouse.
DR PRO; PR:Q8CGF6; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8CGF6; protein.
DR Bgee; ENSMUSG00000040389; Expressed in cortical plate and 223 other tissues.
DR ExpressionAtlas; Q8CGF6; baseline and differential.
DR Genevisible; Q8CGF6; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR006595; CTLH_C.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR040067; WDR47.
DR PANTHER; PTHR19863; PTHR19863; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00668; CTLH; 1.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50897; CTLH; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Developmental protein; Microtubule;
KW Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..920
FT /note="WD repeat-containing protein 47"
FT /id="PRO_0000051398"
FT DOMAIN 10..42
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 45..102
FT /note="CTLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT REPEAT 605..644
FT /note="WD 1"
FT REPEAT 660..699
FT /note="WD 2"
FT REPEAT 707..749
FT /note="WD 3"
FT REPEAT 754..792
FT /note="WD 4"
FT REPEAT 799..838
FT /note="WD 5"
FT REPEAT 841..880
FT /note="WD 6"
FT REPEAT 887..919
FT /note="WD 7"
FT REGION 371..422
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 501..594
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 501..583
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 285
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 289
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 292
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 297
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O94967"
FT MOD_RES 312
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O94967"
FT MOD_RES 423
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O94967"
FT MOD_RES 543
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O94967"
FT CONFLICT 450
FT /note="M -> I (in Ref. 4; AAH40337)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 920 AA; 102312 MW; 401F22FFC00951AA CRC64;
MTAEETVNVK EVEIIKLILD FLNSKKLHIS MLALEKESGV INGLFSDDML FLRQLILDGQ
WDEVLQFIQP LECMEKFDKK RFRYIILKQK FLEALCVNNA MSAEDEPQHL EFTMQEAVQC
LHALEEYCPS KDDYSKLCLL LTLPRLTNHA EFKDWNPSTA RVHCFEEVCV MVAEFIPADR
KLSEAGFKAS NNRLFQLVMK GLLYECCVEF CQSKATGEEI TESEVLLGID LLCGNGCDDL
DLSLLSWLQN LPSSVFSCAF EQKMLNIHVD KLLKPTKAAY ADLLTPLISK LSPYPSSPMR
RPQSADAYMT RSLNPALDGL TCGLTSHDKR ISDLGNKTSP MSHSFANFHY PGVQNLSRSL
MLENTECHSI YEESPERSDT PVEAQQPVSS EAMCQGSGLE KEPANGAQNP VPAKQEKNEL
RDSTEQFQEY YRQRLRYQQH LEQKEQQRQM YQQMLLEGGV NQEDGPDQQQ NLTEQFLNRS
IQKLGELNIG MDSLGNEVPV LNQQCSGSKN NGSNNSSVTS FSTPPQDSSQ RLIHDTANIH
TSTPRNPGST NHIPFHEDSP CGSQNSSEHS VIKPSPGDSS GNLSRSKGEE DDKSKKQFVC
INTLEDTQAV RAVAFHPSGS LYAVGSNSKT LRVCAYPEKM DASAHDNPKQ PVVRFKRNKH
HKGSIYCVAW SPCGQLLATG SNDKYVKVLP FNAETCNATG PDLEFSMHDG TIRDLAFMEG
PESGGAILIS AGAGDCNIYT TDCQRGQGLH ALSGHTGHIL ALYTWSGWMI ASGSQDKTVR
FWDLRVPSCV RVVGTTFHGT GSAVASVAVD PSGRLLATGQ EDSSCMLYDI RGGRMVQSYH
PHSSDVRSVR FSPGAHYLLT GSYDMKIKVT DLQGDLTKQL PLMVVGEHKD KVIQCRWHTQ
DLSFLSSSAD RTVTLWTYSG
