ID   WDR4_DICDI              Reviewed;         467 AA.
AC   Q54UI3;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=WD repeat-containing protein 4 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wdr4; ORFNames=DDB_G0281061;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit mettl1.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; AAFI02000040; EAL66827.1; -; Genomic_DNA.
DR   RefSeq; XP_640793.1; XM_635701.1.
DR   AlphaFoldDB; Q54UI3; -.
DR   SMR; Q54UI3; -.
DR   STRING; 44689.DDB0233974; -.
DR   PaxDb; Q54UI3; -.
DR   EnsemblProtists; EAL66827; EAL66827; DDB_G0281061.
DR   GeneID; 8622847; -.
DR   KEGG; ddi:DDB_G0281061; -.
DR   dictyBase; DDB_G0281061; wdr4.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_585851_0_0_1; -.
DR   InParanoid; Q54UI3; -.
DR   OMA; VSHYPNC; -.
DR   PhylomeDB; Q54UI3; -.
DR   UniPathway; UPA00989; -.
DR   PRO; PR:Q54UI3; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0043527; C:tRNA methyltransferase complex; IBA:GO_Central.
DR   GO; GO:0106004; P:tRNA (guanine-N7)-methylation; IEA:GOC.
DR   Gene3D; 2.130.10.10; -; 2.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 3.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..467
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wdr4"
FT                   /id="PRO_0000370538"
FT   REPEAT          61..100
FT                   /note="WD 1"
FT   REPEAT          164..203
FT                   /note="WD 2"
FT   REPEAT          207..249
FT                   /note="WD 3"
FT   REGION          415..467
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        434..467
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   467 AA;  52703 MW;  F9980366E3C18E9E CRC64;
     MSTTAVFIPP QIMVTSNVEK VPISIITHSS DSNFIAYRLN NKLNIFDNKL NKLGELESGS
     QHTGIIRSIE FTKNNQSLIT SSSDKFLKIW DTTNNFKNIK SINTNKKIIC SILNKDDSEI
     LVSDKCGDVF KYSLIDDSKN KIEVSGDKSA KHDEKESDKN LVFGHYSSIV DIKFSPCFNY
     LLSADRDEKI RVSHYPNCFD IESFCLGHTK YVTEILLVPG RDDLLISGSG DGTIKLWNWK
     QGKCLQTVDF NSKHENAITI PQVVFKVDAT TTTNQLIFSI ENSNNIYILP MNVEKGEFNQ
     SELKTIPLTQ SSPISIDLID NGKTILASLL PTTKEVDVAI AFDSTTLSQQ SDNKVVIAIN
     SVDASTTLKP AELKSVLESI EKKQYRKHVS YSKQMMNSTD NKNKEDDDLS LDEQDIDDTT
     DINNNNKNKN QETIQDSRPL KLRKMTIEGD KEIQKELEEK ESKKQEE