ID   WDR4_XENLA              Reviewed;         396 AA.
AC   Q7ZY78;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=WD repeat-containing protein 4 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wdr4;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Non-catalytic component of a methyltransferase complex
CC       required for the formation of N(7)-methylguanine in a subset of RNA
CC       species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the
CC       methyltransferase complex, it is required to stabilize and induce
CC       conformational changes of the catalytic subunit. Required for the
CC       formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Also
CC       required for the formation of N(7)-methylguanine at internal sites in a
CC       subset of mRNAs. Also required for methylation of a specific subset of
CC       miRNAs. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit mettl1.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH43907.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BC043907; AAH43907.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001080508.1; NM_001087039.1.
DR   AlphaFoldDB; Q7ZY78; -.
DR   SMR; Q7ZY78; -.
DR   DNASU; 380200; -.
DR   GeneID; 380200; -.
DR   KEGG; xla:380200; -.
DR   CTD; 380200; -.
DR   Xenbase; XB-GENE-6253924; wdr4.S.
DR   OrthoDB; 937275at2759; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000186698; Chromosome 2S.
DR   Bgee; 380200; Expressed in oocyte and 19 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043527; C:tRNA methyltransferase complex; ISS:UniProtKB.
DR   GO; GO:0106004; P:tRNA (guanine-N7)-methylation; IEA:GOC.
DR   GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 1.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..396
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wdr4"
FT                   /id="PRO_0000370534"
FT   REPEAT          52..91
FT                   /note="WD 1"
FT   REPEAT          94..133
FT                   /note="WD 2"
FT   REPEAT          137..178
FT                   /note="WD 3"
FT   REPEAT          180..220
FT                   /note="WD 4"
SQ   SEQUENCE   396 AA;  43646 MW;  E0D052953A64BF36 CRC64;
     MLRVGPGSLA ITGGSRLLGH RVGSECCPFH LDCSLLEKQS AAPGQEGSAD SHGSDKILAA
     AFSPSGEYFA LTDDNKRLVL FRTKPAWEKI SVRWVSRRCT ALTFSPCGNH ILVADKSGDV
     FSFSVPRALE QGRLELGHLS MLLDVTVSLD GKHIITCDRD EKIRVSCWGA PHVIMSFCLG
     HTEFVSQLLP LPGQEKLLLS GSGDGTLRLW EYESGKEVHS VTLRSLAHEL EDQENKRFAV
     SRISCCSCNG IQLAVLCEGV PGIFLFSVSP EPRLTFTQYI ALTHTPIDLD FDGSAFLWVL
     SGVREEPLLK YKELDDQWQS VSNDEELTRL TGIIQENWGD LEGAGAPESR FVGLYKAVFD
     NMATYLQKKE LRLESEKRKA ADGQVVLASK VQKTES