ID   WDR4_XENTR              Reviewed;         396 AA.
AC   Q5FVB6;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 {ECO:0000255|HAMAP-Rule:MF_03056};
DE   AltName: Full=WD repeat-containing protein 4 {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wdr4;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Non-catalytic component of a methyltransferase complex
CC       required for the formation of N(7)-methylguanine in a subset of RNA
CC       species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the
CC       methyltransferase complex, it is required to stabilize and induce
CC       conformational changes of the catalytic subunit. Required for the
CC       formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Also
CC       required for the formation of N(7)-methylguanine at internal sites in a
CC       subset of mRNAs. Also required for methylation of a specific subset of
CC       miRNAs. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit mettl1.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH90087.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BC090087; AAH90087.1; ALT_INIT; mRNA.
DR   AlphaFoldDB; Q5FVB6; -.
DR   SMR; Q5FVB6; -.
DR   InParanoid; Q5FVB6; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000008143; Genome assembly.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043527; C:tRNA methyltransferase complex; IBA:GO_Central.
DR   GO; GO:0106004; P:tRNA (guanine-N7)-methylation; IEA:GOC.
DR   GO; GO:0006400; P:tRNA modification; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..396
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wdr4"
FT                   /id="PRO_0000370535"
FT   REPEAT          52..91
FT                   /note="WD 1"
FT   REPEAT          94..133
FT                   /note="WD 2"
FT   REPEAT          137..178
FT                   /note="WD 3"
FT   REPEAT          180..220
FT                   /note="WD 4"
FT   REGION          376..396
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   396 AA;  43559 MW;  14CAD00A5B7A171D CRC64;
     MLRLGSSGLA LTGSSRVLGH RAGSECPPFH LDCSSLEKQS AAPGQEGSVD PQGSDKILAA
     AFSPSGEYFA LTDDNKRLIL FQTKPAWETI SVRWVSRRCT ALTFSPCGAH ILVADKSGDV
     FSFSVRRSRE AGRLELGHLS MLLDVAVSPD GNHIITCDRD EKIRVSRWDS PHVIMSFCLG
     HTEFVSQLLP LPGADKLLLS GSGDGTLRLW DYESGREVQS VTLSGLDLGM ERQENKRFTV
     SRISCCACNG IQLAMLCEGV PGIFLFSVAP GPRLTLTQYV ALTHTPLDLD FDGSAYLWVL
     SGDWEEPLLK YKELNGQWQS VVNDEEVTRL SGFIRENWAD LEGAGAPENR FAVLYKAVVD
     NMATYLQKKE LRLENEKRKT TEGQVAPASK VQKTEC