WDR52_CAEEL
ID WDR52_CAEEL Reviewed; 395 AA.
AC Q93847;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=WD repeat-containing protein wdr-5.2 {ECO:0000305};
GN Name=wdr-5.2 {ECO:0000312|WormBase:K04G11.4};
GN Synonyms=swd-3.2 {ECO:0000312|WormBase:K04G11.4};
GN ORFNames=K04G11.4 {ECO:0000312|WormBase:K04G11.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24682813; DOI=10.1093/nar/gku221;
RA Li T., Kelly W.G.;
RT "A role for WDR5 in TRA-1/Gli mediated transcriptional control of the
RT sperm/oocyte switch in C. elegans.";
RL Nucleic Acids Res. 42:5567-5581(2014).
CC -!- FUNCTION: Sex determining protein required in the germline to promote
CC the spermatogenesis to oogenesis switch during the late larval stages
CC of development. Acts with the sex determining factor tra-1, and
CC redundantly with wdr-5.1, to regulate fog-3 expression, which in turn
CC determines germ cell fate. Not required for methylation of histone H3
CC 'Lys-4'. {ECO:0000269|PubMed:24682813}.
CC -!- DISRUPTION PHENOTYPE: No obvious phenotype at 20 degrees Celsius or 25
CC degrees Celsius. At 20 and 25 degrees Celsius, double knockdown mutants
CC with wdr-5.1 have increased fog-3 expression. At 25 degrees Celsius,
CC these mutants have increased fog-1 expression, reduced brood size
CC accompanied by 42% embryonic lethality with 100% of the surviving
CC progeny being sterile. Surviving progeny display defective
CC spermatogenesis to oogenesis transition with 88% of the gonads only
CC containing sperm. The remaining germ cells in the gonads switch to
CC oogenesis, but the oocytes display either an endoreplication or
CC endomitotic phenotype. Germ cells also have increased expression of the
CC sex determining factor tra-1 in the cytoplasm and as a result there is
CC reduced binding of tra-1 to the fog-3 promoter. No detectable defects
CC in histone H3 'Lys-4' di- or tri-methylation in embryos or adults germ
CC cells at 20 or 25 degrees Celsius. {ECO:0000269|PubMed:24682813}.
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DR EMBL; Z78544; CAB01760.1; -; Genomic_DNA.
DR PIR; T23317; T23317.
DR RefSeq; NP_510394.1; NM_077993.3.
DR AlphaFoldDB; Q93847; -.
DR SMR; Q93847; -.
DR STRING; 6239.K04G11.4; -.
DR EPD; Q93847; -.
DR PaxDb; Q93847; -.
DR PeptideAtlas; Q93847; -.
DR EnsemblMetazoa; K04G11.4.1; K04G11.4.1; WBGene00010572.
DR GeneID; 181540; -.
DR KEGG; cel:CELE_K04G11.4; -.
DR UCSC; K04G11.4; c. elegans.
DR CTD; 181540; -.
DR WormBase; K04G11.4; CE11748; WBGene00010572; wdr-5.2.
DR eggNOG; KOG0266; Eukaryota.
DR HOGENOM; CLU_000288_57_1_1; -.
DR InParanoid; Q93847; -.
DR OMA; IRIWCAR; -.
DR OrthoDB; 957291at2759; -.
DR PhylomeDB; Q93847; -.
DR PRO; PR:Q93847; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00010572; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 7.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 4: Predicted;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..395
FT /note="WD repeat-containing protein wdr-5.2"
FT /evidence="ECO:0000305"
FT /id="PRO_0000051512"
FT REPEAT 105..135
FT /note="WD 1"
FT REPEAT 146..176
FT /note="WD 2"
FT REPEAT 188..218
FT /note="WD 3"
FT REPEAT 230..260
FT /note="WD 4"
FT REPEAT 274..303
FT /note="WD 5"
FT REPEAT 315..348
FT /note="WD 6"
FT REPEAT 360..392
FT /note="WD 7"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 63..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 395 AA; 43128 MW; 49F4D04315F4DC11 CRC64;
MEQRGRASQD EEQAIVGAET GSQPSLPVSP MRPIIAPSPA MVLPTPGHGM GIPQFGPVGL
PAQASTPMLS STPGPSYSSA PTPMPNPSAA NLWPTYKLVA EIPNAHKKSI SGIKFSPDGR
YMGSGSADCS IKIWRMDFVY EKTLMGHRLG INEFSWSSDS KLIVSCSDDK LVKVFDVSSG
RCVKTLKGHT NYVFCCCFNP SGTLIASGSF DETIRIWCAR NGNTIFSIPG HEDPVSSVCF
NRDGAYLASG SYDGIVRIWD STTGTCVKTL IDEEHPPITH VKFSPNGKYI LASNLNNTLK
LWDYQKLRVL KEYTGHENSK YCVAANFSVT GGKWIVSGSE DHKVYIWNLQ TREILQTLDG
HNTAVMCTDC HPGQNIIASA ALEPDMRIKI WRSQS
