WDR54_MOUSE
ID WDR54_MOUSE Reviewed; 334 AA.
AC Q9R0D8; Q3U4A4;
DT 27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=WD repeat-containing protein 54 {ECO:0000305};
GN Name=Wdr54 {ECO:0000312|MGI:MGI:1922909}; Synonyms=D3Mm3e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=9806835; DOI=10.1006/geno.1998.5496;
RA Weber J.S., Jang W., Simin K., Lu W., Yu J., Meisler M.H.;
RT "High-resolution genetic, physical, and transcript map of the mnd2 region
RT of mouse chromosome 6.";
RL Genomics 54:107-115(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: When cross-linked to form dimers and trimers, it has a
CC regulatory effect on ERK signaling pathway activity in response to EGF
CC stimulation. Colocalizes with the EGF receptor in WDR54-specific
CC vesicle where it sustains the internalization and controls the
CC degradation of the EGF receptor after EGF stimulation.
CC {ECO:0000250|UniProtKB:Q9H977}.
CC -!- SUBUNIT: Homodimer and homotrimer; forms tight forms of dimers and
CC trimers. {ECO:0000250|UniProtKB:Q9H977}.
CC -!- SUBCELLULAR LOCATION: Vesicle {ECO:0000250|UniProtKB:Q9H977}.
CC Note=Aggregates in vesicles when cross-linked.
CC {ECO:0000250|UniProtKB:Q9H977}.
CC -!- DOMAIN: WD6 repeat is required for cross-linking by TGM2.
CC {ECO:0000250|UniProtKB:Q9H977}.
CC -!- PTM: Cross-linked to tightly form both dimers and trimers by TGM2.
CC Cross-linking enhances the activation of EGF receptor-mediated
CC signaling pathway. Cross-linking is inhibited by EGF.
CC {ECO:0000250|UniProtKB:Q9H977}.
CC -!- PTM: Ubiquitinated. EGF increases ubiquitination.
CC {ECO:0000250|UniProtKB:Q9H977}.
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DR EMBL; AF053628; AAD54515.1; -; mRNA.
DR EMBL; AK006536; BAB24640.1; -; mRNA.
DR EMBL; AK154345; BAE32529.1; -; mRNA.
DR EMBL; BC083332; AAH83332.1; -; mRNA.
DR CCDS; CCDS20275.1; -.
DR RefSeq; NP_076279.1; NM_023790.2.
DR RefSeq; XP_006506787.1; XM_006506724.3.
DR RefSeq; XP_006506789.1; XM_006506726.3.
DR AlphaFoldDB; Q9R0D8; -.
DR STRING; 10090.ENSMUSP00000116608; -.
DR iPTMnet; Q9R0D8; -.
DR PhosphoSitePlus; Q9R0D8; -.
DR EPD; Q9R0D8; -.
DR MaxQB; Q9R0D8; -.
DR PaxDb; Q9R0D8; -.
DR PeptideAtlas; Q9R0D8; -.
DR PRIDE; Q9R0D8; -.
DR ProteomicsDB; 297893; -.
DR Antibodypedia; 51442; 82 antibodies from 14 providers.
DR Ensembl; ENSMUST00000125894; ENSMUSP00000122873; ENSMUSG00000030032.
DR Ensembl; ENSMUST00000153148; ENSMUSP00000116608; ENSMUSG00000030032.
DR GeneID; 75659; -.
DR KEGG; mmu:75659; -.
DR UCSC; uc009cmv.2; mouse.
DR CTD; 84058; -.
DR MGI; MGI:1922909; Wdr54.
DR VEuPathDB; HostDB:ENSMUSG00000030032; -.
DR eggNOG; ENOG502QRUZ; Eukaryota.
DR GeneTree; ENSGT00390000014530; -.
DR HOGENOM; CLU_045688_0_0_1; -.
DR InParanoid; Q9R0D8; -.
DR OMA; WENYICV; -.
DR OrthoDB; 839593at2759; -.
DR PhylomeDB; Q9R0D8; -.
DR TreeFam; TF329316; -.
DR BioGRID-ORCS; 75659; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q9R0D8; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9R0D8; protein.
DR Bgee; ENSMUSG00000030032; Expressed in olfactory epithelium and 141 other tissues.
DR ExpressionAtlas; Q9R0D8; baseline and differential.
DR Genevisible; Q9R0D8; MM.
DR GO; GO:0031982; C:vesicle; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0002091; P:negative regulation of receptor internalization; ISS:UniProtKB.
DR GO; GO:0042058; P:regulation of epidermal growth factor receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0043408; P:regulation of MAPK cascade; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Repeat; Ubl conjugation; WD repeat.
FT CHAIN 1..334
FT /note="WD repeat-containing protein 54"
FT /id="PRO_0000051416"
FT REPEAT 116..155
FT /note="WD 1"
FT REPEAT 162..206
FT /note="WD 2"
FT REPEAT 208..247
FT /note="WD 3"
FT REPEAT 250..289
FT /note="WD 4"
SQ SEQUENCE 334 AA; 35613 MW; C8F6BC24F9C1EE69 CRC64;
MFRRERSIPL RSSAAALSNN LSVLQLPARD LTHFGVVHGP SAQLLSAAPE GVPLAQRQLH
VKEGAGVSPP LITQVHWCVL PFRVLLVLTS HRGIQMYESD GSVMVYWHAL DSGDASSVQA
MFARGIAASV HFICVGTCSG RVLVFDIPAK GPNIVLSEEL AGHQTPITDI ATERAQGQDG
VADMVTADDS GVLCVWRSGP EFTLLTRIPG FGVPCPSVQL WQGIVAAGYG NGQVRLYDAS
TGALHVQISA HARTISALDL APEVGKLLSA AEDTFVHIWK LNRNPESGSI EVEHCHGECI
SDTQVCGARF CDPLGSSFAV TGYDLAEILR FGTV
