WDR55_ARATH
ID WDR55_ARATH Reviewed; 353 AA.
AC O80775; F4IGY3;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=WD repeat-containing protein 55 {ECO:0000305};
DE AltName: Full=Protein WD repeat domain 55 {ECO:0000303|PubMed:22447688};
DE AltName: Full=Protein WDR55 homolog {ECO:0000305};
GN Name=WDR55 {ECO:0000303|PubMed:22447688};
GN OrderedLocusNames=At2g34260 {ECO:0000312|Araport:AT2G34260};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, INTERACTION WITH DDB1A, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=22447688; DOI=10.1105/tpc.111.089425;
RA Bjerkan K.N., Jung-Romeo S., Jurgens G., Genschik P., Grini P.E.;
RT "Arabidopsis WD repeat domain55 Interacts with DNA damaged binding protein1
RT and is required for apical patterning in the embryo.";
RL Plant Cell 24:1013-1033(2012).
RN [7]
RP FUNCTION.
RX PubMed=23803743; DOI=10.4161/psb.25347;
RA Bjerkan K.N., Grini P.E.;
RT "The Arabidopsis DDB1 interacting protein WDR55 is required for vegetative
RT development.";
RL Plant Signal. Behav. 8:0-0(2013).
CC -!- FUNCTION: Required for male and female gametogenesis, seed development,
CC and embryo and endosperm development at early stages. Involved in the
CC establishment of bilateral symmetry in the transition from the globular
CC to the heart embryo stage. May act in the frame of a CRL4 complex
CC (PubMed:22447688). Required for proper vegetative growth and
CC organization of the adult plant body. May play a role in hormonal
CC control of plant development (PubMed:23803743).
CC {ECO:0000269|PubMed:22447688, ECO:0000269|PubMed:23803743}.
CC -!- SUBUNIT: Interacts with DDB1A. {ECO:0000269|PubMed:22447688}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22447688}. Cytoplasm
CC {ECO:0000269|PubMed:22447688}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O80775-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O80775-2; Sequence=VSP_057867;
CC -!- TISSUE SPECIFICITY: Highly expressed in roots. Expressed in cotyledons,
CC leaves, buds and flowers. {ECO:0000269|PubMed:22447688}.
CC -!- DEVELOPMENTAL STAGE: Expressed in female gametophyte throughout embryo
CC sac development. During pollen development, expressed from meiosis up
CC to the second pollen mitosis, but not at mature (three nucleate)
CC stages. {ECO:0000269|PubMed:22447688}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality when homozygous, due to
CC embryo and endosperm developmental arrest at various stages ranging
CC from the zygote stage to the globular stage. Failure in the fusion of
CC the polar cells in embryo sac development.
CC {ECO:0000269|PubMed:22447688}.
CC -!- MISCELLANEOUS: Plants with a weak allele homozygous for WDR55 display
CC pleiotropic phenotypes in the seedling and adult stages.
CC {ECO:0000269|PubMed:23803743}.
CC -!- SIMILARITY: Belongs to the WD repeat WDR55 family. {ECO:0000305}.
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DR EMBL; AC004481; AAC27402.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08945.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08946.1; -; Genomic_DNA.
DR EMBL; AK117481; BAC42145.1; -; mRNA.
DR EMBL; BT005438; AAO63858.1; -; mRNA.
DR EMBL; AY087666; AAM65204.1; -; mRNA.
DR PIR; T02314; T02314.
DR RefSeq; NP_565782.1; NM_128977.4. [O80775-1]
DR RefSeq; NP_973596.1; NM_201867.2. [O80775-2]
DR AlphaFoldDB; O80775; -.
DR SMR; O80775; -.
DR STRING; 3702.AT2G34260.1; -.
DR iPTMnet; O80775; -.
DR PaxDb; O80775; -.
DR PRIDE; O80775; -.
DR ProteomicsDB; 242636; -. [O80775-1]
DR EnsemblPlants; AT2G34260.1; AT2G34260.1; AT2G34260. [O80775-1]
DR EnsemblPlants; AT2G34260.2; AT2G34260.2; AT2G34260. [O80775-2]
DR GeneID; 817987; -.
DR Gramene; AT2G34260.1; AT2G34260.1; AT2G34260. [O80775-1]
DR Gramene; AT2G34260.2; AT2G34260.2; AT2G34260. [O80775-2]
DR KEGG; ath:AT2G34260; -.
DR Araport; AT2G34260; -.
DR TAIR; locus:2040804; AT2G34260.
DR eggNOG; KOG2444; Eukaryota.
DR InParanoid; O80775; -.
DR OMA; QTHHPHD; -.
DR OrthoDB; 1173229at2759; -.
DR PhylomeDB; O80775; -.
DR PRO; PR:O80775; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80775; baseline and differential.
DR Genevisible; O80775; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009855; P:determination of bilateral symmetry; IMP:TAIR.
DR GO; GO:0080186; P:developmental vegetative growth; IMP:UniProtKB.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0009960; P:endosperm development; IMP:TAIR.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR017422; WDR55.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF00400; WD40; 2.
DR PIRSF; PIRSF038169; WD_repeat_p55; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Nucleus;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..353
FT /note="WD repeat-containing protein 55"
FT /id="PRO_0000433993"
FT REPEAT 4..43
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 49..88
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 92..130
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 133..172
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 175..214
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 218..257
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 260..299
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REGION 300..353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 338..353
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..65
FT /note="MEIDLGANAFGIDFHPSTNLVAAGLIDGHLHLYRYDSDSSLVRERKVRAHKE
FT SCRAVRFIDDGQR -> MFFCWVLG (in isoform 2)"
FT /id="VSP_057867"
SQ SEQUENCE 353 AA; 38426 MW; AC72726AD631D581 CRC64;
MEIDLGANAF GIDFHPSTNL VAAGLIDGHL HLYRYDSDSS LVRERKVRAH KESCRAVRFI
DDGQRIVTAS ADCSILATDV ETGAQVAHLE NAHEDAVNTL INVTETTIAS GDDKGCVKIW
DTRQRSCSHE FNAHEDYISG MTFASDSMKL VVTSGDGTLS VCNLRTSKVQ SQSEFSEDEL
LSVVIMKNGR KVICGTQNGT LLLYSWGFFK DCSDRFVDLA PNSVDALLKL DEDRLITGCD
NGIISLVGIL PNRIIQPIGS HDYPIEDLAL SHDKKFLGST AHDSMLKLWN LEEILEGSNV
NSGNASGAAE DSDSDNDGMD LDNDPSKSSK GSKRKTKSKA NTLNATNNFF ADL
