WDR59_DROME
ID WDR59_DROME Reviewed; 969 AA.
AC Q9VKK2; B5RIT6; Q7KTE5; Q8T9F8;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 3.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=GATOR complex protein Wdr59 {ECO:0000303|PubMed:23723238};
GN Name=Wdr59 {ECO:0000303|PubMed:23723238, ECO:0000312|FlyBase:FBgn0032339};
GN ORFNames=CG4705 {ECO:0000312|FlyBase:FBgn0032339};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A.,
RA Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-373, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [6]
RP FUNCTION.
RX PubMed=23723238; DOI=10.1126/science.1232044;
RA Bar-Peled L., Chantranupong L., Cherniack A.D., Chen W.W., Ottina K.A.,
RA Grabiner B.C., Spear E.D., Carter S.L., Meyerson M., Sabatini D.M.;
RT "A Tumor suppressor complex with GAP activity for the Rag GTPases that
RT signal amino acid sufficiency to mTORC1.";
RL Science 340:1100-1106(2013).
RN [7]
RP IDENTIFICATION IN THE GATOR COMPLEX.
RX PubMed=27166823; DOI=10.1371/journal.pgen.1006036;
RA Cai W., Wei Y., Jarnik M., Reich J., Lilly M.A.;
RT "The GATOR2 component Wdr24 regulates TORC1 activity and lysosome
RT function.";
RL PLoS Genet. 12:E1006036-E1006036(2016).
CC -!- FUNCTION: A component of the GATOR subcomplex GATOR2 which functions as
CC an activator of the amino acid-sensing branch of the TORC1 signaling
CC pathway. The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the
CC TORC1 pathway in order to mediate metabolic homeostasis, female
CC gametogenesis and the response to amino acid limitation and complete
CC starvation. GATOR2 activates the TORC1 signaling pathway through the
CC inhibition of the GATOR1 subcomplex, controlling the switch to cell
CC proliferation and growth under nutrient replete conditions and during
CC female oocyte development. {ECO:0000269|PubMed:23723238}.
CC -!- SUBUNIT: Component of the GATOR complex consisting of mio, Nup44A/Seh1,
CC Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13. Within the GATOR complex,
CC probable component of the GATOR2 subcomplex which is likely composed of
CC mio, Nup44A/Seh1, Wdr24, Wdr59 and Sec13.
CC {ECO:0000269|PubMed:27166823}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL39932.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAZ66312.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014134; AAS64677.1; -; Genomic_DNA.
DR EMBL; AY069787; AAL39932.1; ALT_FRAME; mRNA.
DR EMBL; BT023805; AAZ66312.1; ALT_FRAME; mRNA.
DR EMBL; BT044210; ACH92275.1; -; mRNA.
DR RefSeq; NP_609485.4; NM_135641.4.
DR RefSeq; NP_995682.1; NM_205960.4.
DR AlphaFoldDB; Q9VKK2; -.
DR SMR; Q9VKK2; -.
DR BioGRID; 60602; 1.
DR STRING; 7227.FBpp0079785; -.
DR iPTMnet; Q9VKK2; -.
DR PaxDb; Q9VKK2; -.
DR PRIDE; Q9VKK2; -.
DR DNASU; 34540; -.
DR EnsemblMetazoa; FBtr0080196; FBpp0079785; FBgn0032339.
DR EnsemblMetazoa; FBtr0343622; FBpp0310217; FBgn0032339.
DR GeneID; 34540; -.
DR KEGG; dme:Dmel_CG4705; -.
DR UCSC; CG4705-RA; d. melanogaster.
DR CTD; 79726; -.
DR FlyBase; FBgn0032339; Wdr59.
DR VEuPathDB; VectorBase:FBgn0032339; -.
DR eggNOG; KOG0309; Eukaryota.
DR GeneTree; ENSGT00940000157600; -.
DR HOGENOM; CLU_009370_0_0_1; -.
DR InParanoid; Q9VKK2; -.
DR OMA; HDACIPF; -.
DR OrthoDB; 590848at2759; -.
DR PhylomeDB; Q9VKK2; -.
DR BioGRID-ORCS; 34540; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34540; -.
DR PRO; PR:Q9VKK2; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032339; Expressed in adult Malpighian tubule (Drosophila) and 25 other tissues.
DR Genevisible; Q9VKK2; DM.
DR GO; GO:0061700; C:GATOR2 complex; TAS:FlyBase.
DR GO; GO:0035859; C:Seh1-associated complex; IDA:FlyBase.
DR GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR GO; GO:0035591; F:signaling adaptor activity; IBA:GO_Central.
DR GO; GO:0034198; P:cellular response to amino acid starvation; IMP:UniProtKB.
DR GO; GO:0032008; P:positive regulation of TOR signaling; IMP:UniProtKB.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; IBA:GO_Central.
DR CDD; cd16692; mRING-H2-C3H3C2_WDR59; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR006575; RWD-domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR039456; WDR59_mRING-H2-C3H3C2.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50908; RWD; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..969
FT /note="GATOR complex protein Wdr59"
FT /id="PRO_0000280724"
FT REPEAT 127..167
FT /note="WD 1"
FT REPEAT 172..211
FT /note="WD 2"
FT REPEAT 215..255
FT /note="WD 3"
FT REPEAT 258..303
FT /note="WD 4"
FT REPEAT 307..351
FT /note="WD 5"
FT DOMAIN 435..538
FT /note="RWD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00179"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 373
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 969 AA; 107969 MW; B60C97C86C1F1314 CRC64;
MPPTETLRPG ERGTAGGPGA GAPEQTYIVR QSNKYYEHRD SQATAMSVDY SGQWVLLAGR
GHLALQRLGQ DDGSLRRHER QSKYEVSVAE FAICPSRKEY CAIATSQHID IVRWGTAEPH
YEMSLRGHTR TVTDIDWHGK DPNLLVSCSI DTFSHIWDLR EPRKPALSLN AVCMSGATQV
GFNRVSGNLL AAAHDGDLRI WDIRKGSCPT HYITAHLNRV HGINWSHKRE TCLATASQDG
TVKYFDVCNP RRAEKIITTM SPVWRARYTP IGNGLVSIVV PHLGRGENSL LLWSNSKQTD
PICSFVGHTD VILDFAWRPN RESSNEIELV TWSRDRTLRV WKIDDNMLKL CEPSAEEIES
RFEPDLSELR VPTPPEFLHP RSILVAASLP ISTGDGACNT LPMARSPSFG GGYYRREEPH
IARSLTDQPT CSLHHEFSLL NTNMPHVEVD TLDAIKRYAC FKICAGGHTV ILQVTFTTSY
PSPSAPPDFQ LCQGTTLSSE VSGVLLKVLR CNALQRVKKS RTCLEQCLRA LVAAMKKKVA
AVGGADRSQL LLQSPRLEGA LSSTLHDACI PYPRTSGVHF NAIGMLTTFA QPVNNKRLTL
RQHTALTPRT FSSINGSGLL GNVMATAQRE ANASFYLQER MISGKPGKQR AIRQMNGSPV
VHVYDTSSLL HISRRMAREF SLDKCNIAET CRKNGEICRQ HGRLDLVPVW LLAELIATPQ
IPHETLNDLL FYKDPFKKSL LESLIMHYAI SGDIQTAVLL ACLFDKCPTG GGAIMEKTVR
LPPQLNSQIS PYHTVLPLDV KSSSSSNTWQ QLKQLRSNSW SDSLDLEIKQ IQSDAYACSL
IRRTKMPLFD QFKRAYAEIL FGWQLLSKRA LILKHTQNTP PPVQGVEFVT ECRKCAKPKR
TPKCEPCKRP VLFCVLCRLP VKGAANACLA CGHGGHIDHM MQWFEKHNVC ATCGCKCLER
TSELLALLS
