WDR74_BOVIN
ID WDR74_BOVIN Reviewed; 385 AA.
AC Q58D06; A1L518; A4FUF4; Q0V8D2; Q1JPA8; Q1JPK8; Q58D27;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=WD repeat-containing protein 74;
GN Name=WDR74;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Thymus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Regulatory protein of the MTREX-exosome complex involved in
CC the synthesis of the 60S ribosomal subunit. Participates in an early
CC cleavage of the pre-rRNA processing pathway in cooperation with NVL.
CC {ECO:0000250|UniProtKB:Q6RFH5}.
CC -!- SUBUNIT: Isoform 1 interacts (through WDR repeats) with NVL; the
CC interaction is independent of RNA or pre-60S ribosome particles.
CC Isoform 2 does not interact with NVL. Interacts with MTREX; the
CC interaction dissociation in a late stage of rRNA synthesis is required
CC for appropriate maturation of pre-60S particles and depends on the
CC ATPase activity of NVL. {ECO:0000250|UniProtKB:Q6RFH5}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q6RFH5}. Nucleus {ECO:0000250|UniProtKB:Q6RFH5}.
CC Note=Nucleolar location depends on active PolI transcription of pre-
CC rRNA. {ECO:0000250|UniProtKB:Q6RFH5}.
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DR EMBL; BT021738; AAX46585.1; -; mRNA.
DR EMBL; BT021770; AAX46617.1; -; mRNA.
DR EMBL; BT021791; AAX46638.1; -; mRNA.
DR EMBL; BT021906; AAX46753.1; -; mRNA.
DR EMBL; BT025345; ABF57301.1; -; mRNA.
DR EMBL; BT025445; ABF57401.1; -; mRNA.
DR EMBL; BT026287; ABG81443.1; -; mRNA.
DR EMBL; BT029805; ABM06072.1; -; mRNA.
DR EMBL; BC114802; AAI14803.1; -; mRNA.
DR RefSeq; NP_001014854.1; NM_001014854.2.
DR RefSeq; XP_005226944.1; XM_005226887.3.
DR AlphaFoldDB; Q58D06; -.
DR SMR; Q58D06; -.
DR STRING; 9913.ENSBTAP00000012489; -.
DR PaxDb; Q58D06; -.
DR Ensembl; ENSBTAT00000012489; ENSBTAP00000012489; ENSBTAG00000009491.
DR GeneID; 505875; -.
DR KEGG; bta:505875; -.
DR CTD; 54663; -.
DR VEuPathDB; HostDB:ENSBTAG00000009491; -.
DR VGNC; VGNC:36918; WDR74.
DR eggNOG; KOG3881; Eukaryota.
DR GeneTree; ENSGT00390000015119; -.
DR HOGENOM; CLU_033769_2_0_1; -.
DR InParanoid; Q58D06; -.
DR OMA; YTGTTMG; -.
DR OrthoDB; 1439033at2759; -.
DR TreeFam; TF314666; -.
DR Proteomes; UP000009136; Chromosome 29.
DR Bgee; ENSBTAG00000009491; Expressed in digestive system secreted substance and 108 other tissues.
DR ExpressionAtlas; Q58D06; baseline and differential.
DR GO; GO:0000176; C:nuclear exosome (RNase complex); IEA:Ensembl.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0001825; P:blastocyst formation; ISS:UniProtKB.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; ISS:UniProtKB.
DR GO; GO:0016070; P:RNA metabolic process; ISS:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR037379; WDR74/Nsa1.
DR PANTHER; PTHR16038; PTHR16038; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 2: Evidence at transcript level;
KW Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW WD repeat.
FT CHAIN 1..385
FT /note="WD repeat-containing protein 74"
FT /id="PRO_0000051427"
FT REPEAT 40..80
FT /note="WD 1"
FT REPEAT 83..122
FT /note="WD 2"
FT REPEAT 128..168
FT /note="WD 3"
FT REPEAT 179..220
FT /note="WD 4"
FT REPEAT 224..266
FT /note="WD 5"
FT REPEAT 267..306
FT /note="WD 6"
FT REGION 320..385
FT /note="Required for nucleolar and nuclear location"
FT /evidence="ECO:0000250|UniProtKB:Q6RFH5"
FT REGION 323..385
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..344
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6RFH5"
FT MOD_RES 311
FT /note="N6-methyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q6RFH5"
FT CONFLICT 303
FT /note="G -> A (in Ref. 1; AAX46617/ABM06072)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 385 AA; 42498 MW; 1B93E702F386AC67 CRC64;
MAAAAARWNH VWVGTDTGIL KGVNLQRKQA ANFTASGQPR REEAVSALCW GAGGETQILV
GCADGTVKHF STEEGRFQGQ RQCPGGEGTF RGLAQVDGTL ITCVDSGILR VWTDKDKEAS
SDPVLELRVG PGVCRMRQDP ARPHIVATGG KENALKVWDL QGSEEPLFRA KNVRNDWLDL
RVPVWDQDIQ FLPESQKLVT CTGYHQVRVY DPASPQRRPV LEATYGEYPL TAVTLTPEGN
SVIVGNTHGQ LAEIDLRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIRN
PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNKVPS EDTETDELWA SLEAAAKRKL
PDWEQTQGAL QARRRKKKRP GSTSS
